Gram Essays

the plate for 24 hours. The second step performed was the Gram Stain test. The Gram Stain was used to determine whether the unknown bacterium was gram-positive or gram-negative. First, the unknown sample was smeared onto a slide along with a drop of distilled water. Second, the unknown was air dried and was heat fixed. Third, a series of stains/solution such as crystal violet, iodine, ethanol, and Safranin were used to complete the Gram Stain. During this process each stain/solution was left on

Bacteria from the provided master stock plate were used for the gram stain test. Bacteria were then streak and grown from the master stock plate onto a working plate. After an incubation time of 24 hours at 37°C, the working plate bacteria were then used to perform the catalase and red blood cell hemolysis tests. After conducting the three tests, it was concluded that unknown #5 was Streptococcus agalactiae. Gram Stain Test The Gram Stain test was first performed to differentiate the bacteria based

Abstract This experiment was carried out to determine the species of the unknown organism. Once a choice of the unknown was made a Gram stain was conducted to determine the gram nature and morphology of the organism which was Gram negative bacilli. Based on those results a citrate utilization test was performed resulting in a positive test. Following the flow chart the next test to conduct was a motility test which also had a positive outcome. Lastly, a glucose fermentation test was conducted to

Primary root canal infections are polymicrobial, typically dominated by obligatory anaerobic bacteria. The most commonly isolated microorganisms before root canal treatment include Gram-negative anaerobic rods, Gram-positive anaerobic cocci, Gram-positive anaerobic and facultative rods, Lactobacillus species and Gram-positive facultative Streptococcus species.83 The obligate anaerobes are rather easily eradicated during root canal treatment. In contrast, facultative bacteria such as non-mutans Streptococci

Introduction. Christian Gram created the Gram stain technique in 1884 (K. Rudolph [Clemson University, Clemson, SC], personal communication). Gram used tissue in his first attempt with staining (1). The staining of the organisms allowed for the viewing of structure and determining those components by the viewing of the color that appeared after staining the organism (2). The purpose of this experiment was to identify the organism as gram-positive or gram-negative. The first step in the gram staining procedure

Excitement in Gram Gram is an extremely enthusiastic person which is definitely shown in the passage. There are certainly a couple ways you can tell. You can tell by her personality and her beliefs, and how the author portrays Gram’s beliefs in the story. It also shows how the two things are very alike. Hopefully Gram’s beliefs won’t get her in too much trouble! Gram has an exciting and spunky personality. When you start reading further in the story you can start to recognize what Gram’s belief

Fast Food Noura Adel Al Bader Gust University for Science and Technology Each day one in four Americans visit a fast food restaurant. If you regularly eat fast food it will diminish your health and all consumers should be more aware of that. Every fast food restaurant should put a sign that shows the harmful effect it has on the wellbeing of a human being. Taking care of what you eat should be part of your lifestyle. Fast food advertisements should be strictly controlled, consuming it

CONCLUSION When you put an egg in vinegar, we see that the shell dissolves, but do you ever wonder why? An egg is made mostly out of calcium carbonate which reacts with an ingredient in vinegar, acetic acid. Acetic acid is about 4% of the vinegar and what breaks apart the solid calcium carbonate crystals. The bubbles we see, from the egg, is the carbonate that make carbon dioxide and the other calcium ions float free. This is the equation: CaCO3 (s) + 2 HC2H3O2 (aq) → Ca(C2H3O2)2 (aq) + H2O

energy is more stable than a compound with higher potential energy. Chapter 7, also discusses the focus on the human body energy in nutrition. This section talks about caloric value of the substance, and how one gram of protein are one gram of carbohydrate typically releases about four cal/gram etc. And how much energy is provided from this. Chapter 7 also discusses energy changes in reactions, and how breaking a bond requires energy. Bond

through to be able to correctly identify what bacteria was given. It is important to correctly identify the bacteria because some bacteria are more harmful than others. The gram stain is the first test that should be performed because it helps narrow down the possibilities by telling one whether the bacteria is gram positive or gram negative. After this test is performed, one shall place bacteria on/in Mannitol Salt agar, MacConkey agar, Eosin Methylene Blue agar, Urea agar, Simmon’s Citrate, Purple

completed in lab, a basic understanding of each type of bacteria was used to create a flow chart that would aid the process of identifying the unknown bacteria within the culture. A gram stain that is performed initially will narrow down the types of tests certain bacteria will and will not respond to. In addition to the gram stain, some of the tests that were used include, a catalase test, an Eosin methylene blue (EMB) agar test, a bile esculin test, and a 6.5% sodium chloride (NaCl) test. If the catalase

second ½ of the organism was used for gram staining. The gram stain method was performed on the unknown organism per lab manual page 42 and two gram stain reactions were identified. Organism B was gram positive cocci in grape like clusters. Because organism B was positive I could eliminate Escherichia coli, Enterobacter aerogenes and Proteus vulgaris because these bacteria would be rod shapes. Organism A was gram negative pink rod shaped and because of gram positive morphology I could eliminate

The Unknown Identification Lab was an experiment that provided the opportunity to apply all the tests that were learned in the semester of lab, to identify the two bacterias that remain unknown. Gram- staining and two other tests will be used to identify the unknowns. This experiment is crucial to the understanding of each test, and can benefit in the ability to identify the characteristics of specific bacteria. Having a clearer understanding of the bacteria can further the research of bacteria for

and Methods: Two unknowns where received by the instructor unknown K and C. using the methods learn in lab for identifying them. Beginning with a Nutrient Agar plate T streak to identify morphology and purity once completed and gram stain was performed. Once the gram stain was determined an oxidase test was performed on both organisms a tested used to determine if the bacterium produces cytochrome c oxidase. Thus moving into Gelatin hydrolysis testing a test preformed to identify if the microbes

did after this was the gram stain. The purpose of this was to find my bug’s gram designation and morphology so that I would be working in the right direction. My bug retained the safranin stain which meant it was gram negative. The cells of the bacteria were rod shaped, therefore, their morphology was bacillus. The following test I did was the Oxidase test. The unknown tested negative for this particular test because the oxistrip failed to turn blue shortly

enumerating Salmonella spp. in a food sample. 1.2 Hypothesis To achieve a negative results in food sample with the absence of Salmonella spp. 1.3 Introduction The genus Salmonella belong in the family of Enterobacteriaceae is a group of rod-shaped, Gram-negative, and facultative anaerobic bacteria, which grow with or without oxygen. It is also a non-spore forming and a motile or non-motile bacterium with exception of S.gallinarum and S.pullorum. The genus Salmonella consist of two species, S. enterica

Unknown Paper I Introduction This lab is a presentation of lab tests performed to finalize a conclusion based on results to identify the given unknown bacteria. The unknown bacteria was identified based on lab test results in the table provided in class for the possible unknown bacteria. The unknown bacteria identified as #36, and based on the lab tests is Enterobacter II Materials and Methods Catalase Test- this test determines whether bacteria have the enzyme catalase which catalyzes the

INTRODUCTION Bacterial diseases is any type of disease caused by bacteria. Bacteria 's are a kind of microorganism, which are tiny forms of life that cannot see by easy only be seen with a microscope. The bacteria that cause disease are called pathogenic bacteria. Bacterial diseases happen when pathogenic bacteria inter the body and begin to reproduce and grow in tissues. Pathogenic bacteria may also emit toxins that damage the body. Common pathogenic bacteria and the types of bacterial diseases

the introduction were proven correct but most were not. In the beginning, it was thought that in three plates the bacteria could be separated, however, it actually took four plates to separate the bacteria. It was confirmed that both bacteria are gram positive. However, along with this one of my bacteria was not Staphylococcus. One being Bacillus was confirmed and actually both ended up being Bacillus species. It was thought that one bacteria would be catalase negative and the other catalase

1. Describe the main characteristics of Alcaligenes faecalis. Where is this bacterium naturally found? How large is the genome of Alcaligenes faecalis? Alcaligenes faecalis is an aerobic nonfermentative, oxidase-positive, nonencapsulated, gram-negative rod. It is so named for its ability to produce an alkaline reaction in certain media. Microscopic analyses revealed that the bacteria are located in the esophagus and intestine of the nematodes Steinernema feltiae, S. carpocapsae, and H. bacteriophora