Every organism in the universe requires water for its survival. Arguing out that water is good or bad is inconclusive. Water for human consumption is supposed to be subjected to quality tests so as to determine its safety for consumption and usage. A number of water sources around the globe suffer from some form of impurities. This makes it unsafe and unhealthy for individuals to use it. Challenges such as unsafe water, insufficient or the lack of quality healthy water has been an issue which has
Lactobacilli are lactic acid bacteria, an anaerobic / microaerophilic, non-sporulation, low in G+C content gram-positive bacteria which belong to the phylum Firmicutes [1]. Lactobacillus colonizes the human gut and confers various health benefits to humans. Lactobacillus acidophilus NCFM (North Carolina Food Microbiology) strain is marketed as probiotic microbe which is routinely used in dietary supplements and fermented dairy products [2]. L. acidophilus is naturally occurring bacteria in human gut, intestines
the lab was to put into action the methods that have been learned in the laboratory to determine our unknown bacteria. Bacteria’s can have different features, shapes, and or arrangements that help microbiologist determined their role in our life (whether they are good or bad for humans). Bacteria can be classified as gram positive or negative (difference in call wall). Gram positive bacteria have a thick cell wall of peptidoglycan (“polymer of disaccharides cross-linked by shorts chains of amino acids”)
different types of media plates affect the growth of skin microflora mainly microflora in the nostril It was hypothesized that the nostril microflora were gram-positive bacteria that belong to the genera Streptococcus, Staphylococcus and Propionibacteria. Results showed that the nostril microfloras were gram-positive (stained purple). However, gram staining was not enough to prove that the bacterium obtained from the nostril are Streptococcus, Staphylococcus, and Propionibacteria spp. The results do
identify a specific bacterial species is an important skill. In order to discover and being able to identify any microbial bacteria, there a list of test one must perform in order to come with the right microorganism. It is fundamental to be aware of the risk of toxify, the resistance to antibiotics and determining how to prevent its growth and being able to destroy this bacteria. By being able to run both physical and chemical test to determine the identity of the mystery microbe is a unique and useful
different bacteria was selected. The sample contained one gram-positive, one gram-negative paracolon, and one gram-negative coliform. The purpose of the experiment is to identify each of the three species that the mixture contained. After receiving an unknown mixture, the sample was streaked for isolation onto TSA, blood agar, and MacConkey plates. Each plate serves as a first step to identify the unknowns. The TSA (tryptic soy agar) can be used to do a gram stain, which differentiates gram-negatives
Discussion Bacteria from the provided master stock plate were used for the gram stain test. Bacteria were then streak and grown from the master stock plate onto a working plate. After an incubation time of 24 hours at 37°C, the working plate bacteria were then used to perform the catalase and red blood cell hemolysis tests. After conducting the three tests, it was concluded that unknown #5 was Streptococcus agalactiae. Gram Stain Test The Gram Stain test was first performed to differentiate the
activities of different bacteria. Bacterial physiology differs from one species to the other. These differences in carbohydrate metabolism, protein metabolism, fat metabolism, production of certain enzymes and ability to utilize a particular compound help them to be identified by the biochemical tests. Gram’s stain was originally devised by histologist Hans Christian Gram in 1884. Gram-positive bacteria stain purple, while Gram-negative bacteria stain pink when subjected to Gram staining. Approximately
tube and plate to inoculating the bacterium and incubating. After incubated and grown the morphology was observed and several Gram stains were performed to determinate if the bacterium were gram positive or negative, and the morphology of the bacterium. The Gram Stain of my major unknown #202 was determinate to be Gram negative bacilli, and was double checked by the Gram check slide. Also I noticed that my bacterium was a facultative anaerobe and according to my results of endospore test, my bacterium
was collected and necessary test were performed to determine what pathogen or bacteria was the cause of Terrance 's infection. In order to have a better understanding of what they are working with and how to
the field of microbiology so important, especially in infectious disease control. Using the testing procedure laid out by the microbiology field we are able to identify unknown bacteria present in our everyday lives, and along the way learn a lot about their characteristics that separate them from other types of bacteria. Being able to do this is vital in order for us to understand why microbes are present in certain places, how they are able to grow and what restricts their growth, that way they
Anti-infection agents are utilized to treat diseases caused by bacteria mostly Gram-negative bacteria. Nalidixic acid works by killing the microbes that are causing a contamination or infection. It does this by entering the bacterial cells and restraining a bacterial protein called DNA-gyrase. This catalyst is associated with duplicating
appropriate bacterial culture using the quadrant streak plate method 3. This will result in the isolation of individual colonies. Starch Amylose / Amylopectin Test The results for this test on the Unknown 361 were positive. Procedure: 1. Use a fresh (16- to 18-hour) pure culture of test bacteria as an inoculation source. 2. Pick a single isolated colony and either single streak or spot inoculate the surface of the agar medium. 3. A single starch agar plate can be divided into four quadrants for four
After a gram stain was done unknown #257 was identified as a gram positive organism because when observed under the microscope the organism appeared purple with cocci in clusters. The organism was also catalase positive which means that it produced enzyme catalase and bubbled when hydrogen peroxide was added to it. Three test were conducted based on the result of the gram staining procedure. Blood agar with a Novobiocin disk was chosen as well as DNase (DNA) and Mannitol Salts (MSA) agar. The Blood
unknown bacteria # 710 broth had to be successfully isolated on an EMB and MAC agar plate. Using aseptic technique by sterilizing the wire loop with Bunsen Burner between inoculations and flaming the opening of the test tubes before inserting in the loop with the bacteria. The streaking technique used was to isolate the colonies on the agar plates. In addition, the streak plates had to be incubated in a upturned position for 24 hours in a hot temperature incubator at 37 degree Celsius. Bacteria need
Stomach evacuation experiment The experiment was designed to estimate the stomach evacuation rate of capelin eggs in red king crabs at an ambient temperature of 2.9°C, which is within the range of bottom temperature at capelin spawning sites along the coast of northern Norway (Sætre & Gjøsæter 1975). The experiment ran between March 27 and April 6 2011. The collection of experimental animals and capelin eggs is described in Mikkelsen (2013). To obtain at least one datum per hour of digestion time
know, bacteria is a very complex organism and the subject can be very broad. In this essay, the focus will be on bacteria and the bacterial cell structure. Different forms of bacteria, its pros and cons, the cell structure, diseases and resistance will be explained and listed. First bacteria and cell structure is explained, and then moving on to different bacterial forms and diseases, and how diseases can be prevented or even cured. Then finishing the essay will be the conclusion. Bacteria The
polymicrobial, typically dominated by obligatory anaerobic bacteria. The most commonly isolated microorganisms before root canal treatment include Gram-negative anaerobic rods, Gram-positive anaerobic cocci, Gram-positive anaerobic and facultative rods, Lactobacillus species and Gram-positive facultative Streptococcus species.83 The obligate anaerobes are rather easily eradicated during root canal treatment. In contrast, facultative bacteria such as non-mutans Streptococci, Enterococci, and Lactobacilli
Predation in the field This study confirms that invasive red king crab fed on both capelin eggs and capelin off the coast of Finnmark, Norway. Capelin was found in 81.6% and 21.7% of the analysed red king crab stomachs in 2005 and 2006, respectively, while capelin eggs were found in 23.2% and 10.0% of crabs in 2005 and 2006, respectively. These results are in accordance with a previous study where 19% of the analysed red king crab stomachs were reported to contain capelin eggs (Anisimova et al. 2005)
The six bacteria used in this lab were, Citrobacter freundii, Bacillus subtillis, Enterobacter aerogenes, Staphylococcus epidermis, Enterococcus durans, and Escherichia coli. Citrobacter freundii is a Gram-negative rod shape bacteria. The MSA plate will grow Citrobacter freundii, Bacillus subtillis, Enterobacter aerogenes and will have a yellow color change while Staphylococcus epidermis will not grow nor have a color change to yellow. The MacConkey agar will have growth with Escherichia coli, Enterococcus