Keywords: HPTLC, Tetracyclines, residues and broiler meat, validation Introduction Tetracycline antibiotics (TCs) which are represented by oxytetracycline (OTC) and tetracycline (TC), are active against gram positive and negative bacteria antimicrobials and are commonly used in livestock and poultry for prevention and treatment of various diseases [1]. Chemical names for these antibiotics are 4-(Dimethylamino)-1,4,4a,5,5a,6,11,12a-octahydro3,5,6,10,12,12a-hexahydroxy-6-methyl-1,11-dioxo-2-naphthacenecarboxamide
One progress on TLC called high performance TLC (HPTLC; Sherma and Jain, 2000).HPTLC makes use of gel qualities that are finer, so that thinner plates and smaller. This allows faster separation times and better separation efficiency. HPTLC has improved reduced resolution and detection limits, so that the to walk two dimensions. To phospholipids visible on the TLC plates are used detection reagents
Extraction of β-caryophyllene Ground cloves will be used to isolate β-caryophyllene via steam distillation. 5g of ground cloves will be taken in a 500mL RBF(Round bottom flask) with boiling stones, 40mL of dH2O, and 3 to 4 drops of an antifoaming agent (to prevent violent boiling). Then, the contents of the flask will be heated on a heating mantle for an hour and followed by condensation of the distillate through a water jacket. Then allow it to collect in a graduated cylinder. Steam distillation
flavonoids, alkaloids, terpenoids, quinones, phenols, and tannins (Table 11). Alkaloid was seen in ethyl acetate and methanol extracts while terpenoid was noticed only in the chloroform extract. Flavonoid was present only in the methanol extract. HPTLC fingerprinting of ethanol extract of P. zeylanica yield the following results. The Rf values and colour of the spots were recorded (Table 25). Photodocumentation under 254 nm gives 6 spots (Fig 16a), 14 spots under 366 nm (Fig 16b), and 9 spots under
Traditionally, x-ray based instruments and trained animals are the basic choices to handle the explosives substances like TNT. Canines are still considered the gold standard for field methods of detecting explosives [2, 3]. Other techniques like HPTLC [6], HPLC [7], IR [8] & Raman spectroscopy
Chromatographic analysis: Thin Layer Chromatography175 Procedure Thin layer chromatography is used for the separation, identification and quality control of drug. It also determines small amount of impurities or adulterants if present. Apparatus Glass plates of uniform thickness, 15-20 cm long and wide, spreader, chromatographic chamber of glass with a tightly fitting lid having suitable size to accommodate the glass plates and capillary, ultraviolet light source emitting short 254 nm and long
INTRODUCTION: Drotaverine is chemically1-[3,4 –diethoxyphenly)methylidene]-6,7-diethoxy-3,4-dihydro-2H-isoquinoline:hydrochloride[1] and empirical formula is [C24H31NO4.HCL][4] has no anticholinergic effects and is used as antispasmodic drug.specific used for smooth muscle spasm and pain. Chemically Ethamsylate is N-ethylamine2,5dihydroxybenzenesulphonate [2]. belongs to class of haemostatic compound that blocks prostacycline synthetase, an enzyme that converts arachidonic acid to Prostacycline
. DRUG PROFILE 2.1 IBUPROFEN Ibuprofen chemically known as 2-[4-(2-methyl propyl) propanoic acid. It is a propanoic acid derivative classified under non steroidal anti inflammatory agent (NSAID) with analagesic and antipyretic properties.The empirical formula of ibuprofen is C13H18O2 its molecular weight is 206.29. It is indicated for relief of the signs and symptoms of rheumatoid arthritis and osteo arthritis for relief of mild to moderate pain and also indicated for the treatment of dysmenorrheal