Plasmid Essays

  • E. Coli Transformation Lab Report

    506 Words  | 3 Pages

    occurs within plasmids, which are closed circular molecules made up of double stranded DNA. The function of the plasmid is to provide bacteria with genetic advantages such as antibiotic resistance. In this lab, the plasmids provided the ampicillin resistance and the fluorescence. If the bacterial cells are grown in the presence of the antibiotic ampicillin then only the cells that took up the plasmid have the resistance gene. As a result the resistance gene will have to keep the plasmid and the GFP

  • Horizontal Gene Transfer In Eukaryotic Analysis

    518 Words  | 3 Pages

    virus that infect bacteria or a plasmid, which is a circular piece of DNA that exist and

  • Pglo Transformation

    1374 Words  | 6 Pages

    Bacterial transformation is a technique widely practiced by scientists for research purposes. This experiment explored the transformation of E. coli cultures with pGLO plasmids to allow the bacterial cells to express a foreign protein and emit a fluorescent glow under UV light. The transformation was completed through the heat shock method. Both transformed and untransformed E. coli cultures were grown in four mediums. The four mediums were made of different combinations of the LB nutrient broth

  • Gene Transfer Lab Report

    1030 Words  | 5 Pages

    Transfection: One of the methods of gene transfer where the genetic material is deliberately introduced into the animal cell in view of studying various functions of proteins and the gene. This mode of gene transfer involves creation of pores on the cell membrane enabling the cell to receive the foreign genetic material. Transfection can be carried out using calcium phosphate (i.e. tricalcium phosphate), by electroporation, by cell squeezing or by mixing a cationic lipid with the material to produce

  • E. Coli Bacteria Lab Report

    617 Words  | 3 Pages

    In this lab, genes for a fluorescent green protein (GFP) and antibacterial resistance (ARG) were inserted into E. coli bacteria. E. coli bacteria was resuspended in an ice-cold CaCl2 solution. DNA containing GFP and ARG was added to half of the cells before they were “heat shocked” in an ice bath and hot water. The heat shocking made the bacteria’s cell membrane more porous, so the DNA could enter. Recovery broth was added to the cell suspension, and the bacteria was placed in warm water for about

  • Practicum Lab Report

    1757 Words  | 8 Pages

    Introduction The practicum has been developed in RIKEN Centre of Developmental Biology in Kobe, in the laboratory of Axial Pattern Dynamics under the supervision of Inomata-sensei and Matsukawa-san. In the laboratory they try to artificially regulate the gradient shape, they can control morphogen-dependent pattern formation. In general, the shape of a gradient is defined by three factors; synthesis, diffusion, and degradation of morphogen. So, they attempt to spatiotemporally regulate the gradient

  • Dna Restriction Lab Report

    1724 Words  | 7 Pages

    restriction map for the unknown plasmid A. The plasmid A was digested with enzymes BAMH1, PstI, and ScaI and then the resulting fragments were run through an agarose gel via electrophoresis. From the gel electrophoresis and deriving an equation by plotting the log of the size of the DNA size markers and distance migrated, a restriction map was constructed. The restriction map showed that the plasmid has only one ScaI site, which supports that vector PRSETB, is present in the plasmid. From the gel electrophoresis

  • Pglo Lab

    1396 Words  | 6 Pages

    inserting a gene into an Escherichia coli bacteria with the help of a plasmid. Escherichia coli bacteria also known as E. coli, is a bacterium that is rod shaped and contains flagella to help it move. The bacterial DNA is circular inside of an E. coli bacterium. E coli. is most known for being found in the intestine of humans and animals but it can also be found in other places such as food

  • Pglo Transformation Lab Report

    1128 Words  | 5 Pages

    used a procedure called heat shock, accompanied by a bacterial plasmid vector, to transform bacteria with a gene that codes for GFP (Green Fluorescent Protein). A vector is an agent “employed to transfer the gene from one organism to another” (Lab manual). Two common vectors are phages and plasmids. Phages are viruses that infect bacteria. Plasmids are “small circular pieces of DNA found within bacteria cells” (Lab manual). Plasmids “often contain genes for traits that are beneficial to the bacteria

  • Ap Biology Synthesis Essay

    1341 Words  | 6 Pages

    illustration is to consider this as a version of bacterial sex. In conjugation the two bacterial cells connect, and the male donates a piece of DNA to the female. The piece of DNA was excised from a bacterial chromosome. The pieces are called plasmids. Plasmids are like viruses in that they pass out of one cell and into another, but they have no protein coat or "life cycle" differing from that of their host cell. Transduction: In transduction, a virus takes a piece of DNA from the bacterial

  • Griffith's Transformation Principle

    1773 Words  | 8 Pages

    Abstract The transformation principle suggests that bacteria use DNA as their genetic material and are able to exchange their genetic material via a process of transformation. Griffith had theorised the concept of the transformation principle using two strains of bacteria and studied their ability to recombine. Avery and MacLeod followed his studies and suggested DNA was sensitive to DNase, and that the enzyme would destroy the bacteria's ability to exchange genetic material and transform into a

  • Dilution Series Lab Report

    739 Words  | 3 Pages

    2. You have been asked to set up a dilution series, and then use spread plates to determine the viable cell count. Why is it necessary to use a dilution series when isolating bacteria from a biological sample using spread plating? [5 MARKS] It is vital to use a dilution series to reduce the concentration of the original biological sample so it is easier to count the number of isolated colonies which are present on the spread plate. The diluted samples will have a workable number of colonies on

  • Pglo Lab Report

    465 Words  | 2 Pages

    Genetic engineering is changing the DNA code to express different traits. A plasmid is a circular piece of DNA that contains important genetic information. Recombinant DNA is the product after inserting your desired genes. The genes we hoped to insert in the pGLO lab were the GFP gene and the ampicillin resistance gene. GFP was needed so that we would tell if the ampicillin resistance gene had been properly placed when the bacteria glowed under a UV light. The purpose of this lab was to perform a

  • The Pros And Cons Of Counting The Bacteria

    364 Words  | 2 Pages

    Counting the bacteria means determining the number of bacteria in a specific sample. There are many ways that we can use to count the microorganisms, one of which is the plate count. The plate count technique is used to count only the living bacteria by counting the number of colonies.Due to the large number of bacteria, and to be able to count it, the bacteria should be diluted several times and spread on an agar plate. In this way, colonies can be counted. These colonies are called colony forming

  • Unit 2 Plasmid Research Paper

    735 Words  | 3 Pages

    Upload the assignment prior to the beginning of your next lab section. Make sure you give yourself time to troubleshoot any issues you may have with uploading the assignment. You are responsible for uploading the correct file. Given the map of the plasmid in Figure 10-3, you should be able to predict the length of DNA fragments that will result when these digests are completed. Predict sizes of DNA fragments produced from PvuII digest: 628-306= 322 2686-322= 2364 Predict sizes of DNA fragments produced

  • E. Colo Transformation Lab Report

    595 Words  | 3 Pages

    In this experiment, we want to clone SOD gene into pUC19 plasmid of E.coli. Before ligating the target gene into the plasmid, the host cell needs to be competent to accept the vector. E.coli is not a naturally transformable bacterium thus; it needs to become transformable by chemical transformation. This technique is very useful to make competent cells that later will be used for transformation experiment in a short time. Actually there are not so much difference between chemically competent cells

  • Double Digestion Experiment

    1789 Words  | 8 Pages

    1) Present the gel electrophoresis image of plasmid digestion experiment with the correct label. Describe the results of the experiment and list down possible problem that you observe based on the electrophoresis image. Plasmid P1-1 and P1-2 undergo single digestion. Plasmid P1-3 undergoes double digestion. Single digestion is only one restriction enzyme which has been used to digest a DNA. Double digestion is there are 2 restriction enzymes which have been used to digest a DNA. Based on the result

  • Ligation Lab Report

    2038 Words  | 9 Pages

    Ligation The objective of this experiment was to ligate EGFP DNA inserts into pET41a(+) plasmids. A total of five ligations were performed, two actual ligations and three control ligations. The following reagents were utilized: Nco I/Not I cut pET-41a(+) DNA 50 ng/μL, EGFP cDNA insert 7 ng/μL, uncut pET-41a(+) DNA/EGFP recombinant plasmid DNA 25 ng/μL, ligase buffer 10X, and ligase. To prepare ligation #1, a 1:1 molar ratio of pET41/EGFP, 2 μL digested pET41a(+) DNA, 1 μL EGFP cDNA insert, 14 μL

  • Tn 4351 Unit 5 Lab Report

    506 Words  | 3 Pages

    carried an unstable chimeric plasmid, pSS-2. The mobilization of pSS-2 from onestrain of E. coli

  • Genetic Engineering In Medicine

    805 Words  | 4 Pages

    nutritional value and even to the medicine that we consume. Genetic engineering began in 1973 when Herb Boyer and Stanley Cohen created the first recombinant deoxyribonucleic (DNA) organism. They transferred antibiotic resistance genes into a plasmid. The plasmid was later introduced into Escherichia Coli. They then discovered that the following generations of daughter cells were also resistant to the antibiotics. In 1974, Rudolf Jaenisch created the first transgenic animal. After he introduced the