Serial Dilution Many of the laboratory procedures involve the use of dilutions. It is important to understand the concept of dilutions, since they are a handy tool used throughout all areas of the clinical laboratory. These dilutions have to be considered as they make a quantitative difference in what is going on. nA serial dilution is any dilution where the concentration decreases by the same quantity in each successive step. If an answer contains a one/10 dilution the quantity represents 1 a part
First part of this experiment was to create and plan out a dilution series to figure out the concentration of Salmonella enterica in the original solution. Three plates were labeled 1,2, and 3. Plate 1 being the most diluted mixture and 3 being the least diluted mixture. These plates were examined to figure out the CFU. The dilution factors of the samples were 1:30,000 for plate 1, the second plate was 1:60,000, and the third and final plate was 1:120,000. 100uL of each sample was pipetted onto the
Aim The aim of the experiments to be carried out is to determine the kinetic parameters, Km and Vmax, of Alkaline Phosphatase. Theory, Principles and Application of Principles Enzymes are a huge varying group of proteins which are needed to carry out essential metabolic functions in cells. Substrate-specific enzymes, like Alkaline Phosphatase, act as catalysts lowering the needed activation energy to convert the substrate to product. Enzymes are made up of amino-acids and amino-groups have side chains
Calculations Preparation of 1.5μmol/L mixed low-level standard dilution 150μmol/L × V1=1.5μmol/L × 10ml V1=(1.5μmol/L×10ml)/(150μmol/L)=0.1ml Conversion of milliliters to microliters (0.1ml×1000)μL= 100μL Preparation of 3μmol/L mixed low-level standard dilution 150μmol/L × V1=3μmol/L × 10ml V1=(3μmol/L×10ml)/(150μmol/L)=0.2ml Conversion of milliliters to microliters (0.2ml×1000)μL= 200μL Preparation of 3μmol/L mixed low-level standard dilution 150μmol/L × V1=7.5μmol/L × 10ml V1=(7.5μmol/L×10ml)/(150μmol/L)=0
In Tania Modleski’s “The Search for Tomorrow in Today’s Soap Operas” she introduces that the rare appearance and function of soap opera in two ways. The first being the viewer’s ability to occupy the text’s recommendation of the viewers position of ‘the good mother’, and the second using the stereotype ‘villainess’ to displace one’s own bottled up anger and powerlessness. I will analyze these two functions by using Modleski’s perspectives on the positions of soap operas, how a new genre of reality
melodramatic elements, but what is it specifically that makes both of these shows known as a classic example of TV melodrama? To best describe what makes a series like Knots Landing both a melodrama and a serial TV show we will look at two episodes. As a series the show perfectly follows the serial format you find in both TV soaps and modern hit shows. For example the series as a whole deals with multiple protagonists, each of them have their different storylines and dilemmas that fall upon them in
Sarah Koenig, the host and producer of the popular podcast “Serial” is known for her engaging storytelling style and ability to use rhetorical devices and tools of persuasion to keep listeners interested and invested in the story she is telling. As a journalist and narrative storyteller, Koenig’s goal is to present a well-researched and balanced account of the events she is discussing and to use her skills as a writer and communicator to convey that information in a way that is compelling and engaging
Serial is an investigative journalism podcast hosted by Sarah Koenig, narrating a nonfiction story over multiple episodes. The series was co-created and is co-produced by Koenig and Julie Snyder and developed by This American Life. It takes an unorthodox approaches to storytelling that its creator Sarah Koenig employs, and, given the podcast’s massive success, it seems that the public is hungry for these new kinds of narratives. Serial and podcasts are growing in population because of its access
An Analysis on Pornographic Experience in William S. Burroughs’s Naked Lunch William S. Burroughs (1914-1997) was more than just a homosexual who got away with murdering his second wife, Joan Villmer, after shooting her in the head. However, William Burroughs’s misogyny, misanthropy, and drug addiction flavour the literary works that made Burroughs a significant figure in American letters in the twentieth century. Burroughs was a renowned novelist, predominant member of the Beat movement. Though
Apathetic Demeanors Many people in today’s society view psychopaths and sociopaths as ruthless serial killers who are menaces to the peace of society. Albert Camus’s novella, The Stranger, provides another look at a psychopathic and sociopathic characteristics in a person. Meursault, the protagonist in Camus’s novella, exhibits psychopathic and sociopathic tendencies; although Meursault displays both, he is more closely aligned with a sociopath which eventually leads to his execution. Meursault’s
Josef Mengele was infamous for killing hundreds of twins along with people with irregularities. He was nicknamed the angel of death because on occasion unexpected people would be chosen for his devious experiments. With his specially chosen specimens he performed horrid experiments even after their death. Though Josef Mengele did some terrible experiments he is a very important part of history and helps better understand the holocaust. Josef Mengele was born on March 16, 1911 in Gunzburg, Bavaria
Many readers often have a different opinion upon what makes a great hero. The hero Heracles, and Theseus are two classic myths read by readers around the whole school. The myth of Heracles distinguishes a boy who kills monsters/serpents to protect himself in a young age. However, the myth of Theseus distinguishes the life of an ordinary boy, who tries every day to push a rock to discover the rightful identity of his father. While reading the myth of Heracles, and Theseus we can clearly identify
Cults bring fear in the hearts and eyes of the public, but do citizens know how Jim Jones managed to seduce so many people to join his cult, the Peoples Temple? The Peoples Temple was previously loved in the public eye, as they aided anti-segregation groups and community work, but were eventually relocated in Jonestown, Guyana, under the paranoid eyes of Jim Jones, where they were found dead in a mass-suicide. The ways Jim Jones managed to bring in as many followers as he did are still widely debated
Buffalo Bill, this nick name started as a joke in Kansas City Homicide. They said, “this one likes to skin his humps”. Bill is a cruel and crazy killer. He thought he was the transvestist, but he just immersed in his desire completely. He is greedy and vicious. I think it maybe vent his morbid and depress to the world. It is the result of lacking love in his childhood. He wants to be a woman, and search the maternal love from himself. He killed five women, and skin them to sew the cloth for himself
Little Red Riding Hoodie Once upon a time, but not so very long ago, I was the lead witness in a murder. Everyone thinks Little Red Riding Hoodie was the girl’s name, but the story is really about me. I came to know the girl when I was given to her for Christmas. Her favorite color is red like the color of her favorite sports team, the Wisconsin Badgers. She needed something warm to wear while she was riding the bus to school. I provided her that warmth and protection from the bitter Wisconsin
The Murder of the Hollywood Starlet A young hollywood starlet was brutally murdered in 1947 with no killer identified to this day. This is real case that still puzzles investigators today. The woman in question was named Elizabeth Short, but is more widely known as the “Black Dahlia.” She was given this nickname by the press because of the sheer, black clothing she tended to wear (“The Black Dahlia Murder - Read All about it in FBI Records.”). Her body was placed in an empty lot within the area
There are some serial killers who never get caught. Turn of the century killer Jack the Ripper is a famous example. Between 1888 and 1891 an unidentified man preyed on impoverished prostitutes in the East End of London; brutally killing them and butchering their bodies. There are dozens of theories about who he might have been but no-one was ever arrested for the murders. The Zodiac was another serial killer who was never apprehended. He terrorized the people of northern California during the late
Nora Nadya 111711133115 Biopsikologi Perilaku C-1 Draft II (Group 1) Topic: Bad and Good Guy: Why people can be a cold blooded murderer? Cold-Blooded Murderer: Are they genetically inherited? Did you ever hear about cold-blooded murderer cases? Not only in novels or movies but the real cold-blooded murderer cases that were actually happened in real life. For example, the case of Ryan from Jombang, East Java. He was known killed 11 people and he even mutilated his victims in 2008.
Ian Brady and Myra Hindley were two of the most savage murderers ever to live. The press dubbed Myra Hindley, “the most evil woman in Britain.” They murdered and tortured five children aged between 10 and 17 in the years from 1963 to 1965. Brady loved the Saddleworth Moors, close to Manchester in the north of England, and would often go up there. He knew his way around them very well. Initially, the Police knew of only three of their victims: Edward Evans, Lesley Ann Downs, and John Kilbride
Roger Rabbit never looked so suave (Who framed Roger Rabbit?) (1988) Cast: Bob Hoskins, Christopher Lloyd, Charles Fleischer, Joanna Cassidy, Kathleen Turner Director: Robert Zemeckis Synopsis: Los Angeles, 1947. A toon-despising private detective Eddie Valiant (Bob Hoskins) is hired to investigate the murder case of Marvin Acme (Stubby Kaye), the head of Maroon Cartoons and of Toon Town, with the prime suspect being Roger Rabbit (Charles Fleischer), the husband of the voluptuous Jessica Rabbit