Size exclusion chromatography Essays

The purpose of this experiment is to analyze and identify specific proteins from a mixture of proteins using the purify technique of size exclusion chromatography. The use of size exclusion chromatography also called Gel filtration allows to separate proteins according to size (molecular weight) and shape. The idea of separating molecules base of the physical sizes depends on the stationary phase and the mobile phase. Selecting the appropriate stationary phase and mobile phase is vital to obtain

In this experiment, we had purified LDH isozyme from the clarified homogenate via the ammonium sulfate precipitation, affinity chromatography and size exclusion chromatography. Based on the result shown on the LDH table and on the SDS page, the most effective LDH purification step is the affinity chromatography. Even though the affinity purification step recovers the least amount of LDH (only 3% of LDH from the previous 65% cut), it shows the most purified enzyme without any contaminating proteins

INTRODUCTION CHROMATOGRAPHY Chromatography was originally developed in the year 1903 by the Russian botanist Michael Tswett in percolating a petroleum ether extract through a glass column packed with powered calcium carbonate for the separation of colored pigments. Elution means a chromatographic separation involves the placing of the sample into a liquid or solid stationary phase and passing a liquid or gaseous mobile phase through or over it. Whether

Affinity Chromatography using the natural properties of protein Introduction The motivation for developing affinity chromatography is clear and simple, it is because protein has to purify before research studied its structure and mechanism of its’ functions, and each protein has various sizes, mass, pH value, and solubility. However, it is a difficult to separate target protein through single method in “five decades ago” .1 During the early time, scientist used less efficient method to separate

stirred for the appropriate reaction times at 80 °C (Table 2). After completion of the reaction (monitored by thin-layer chromatography, TLC), the mixture was diluted with Et2O and filtered. The organic layer was washed with 10% NaHCO3 solution and then dried over anhydrous Na2SO4. The solvent was evaporated under reduced pressure and the product purified by column chromatography on silica gel to give the corresponding pure aryl

Gellan gum is an anionic deacetylated exocellular polysaccharide secreted by Pseudomonas elodea with a tetrasaccharide repeating unit of one á-L-rhamnose, one â-D-glucuronic acid and two â-D-glucuronic acid residues 30. It has the propensity of gelation which is temperature reliant or cations induced .This gelation involves the formation of double helical junction zones followed by aggregation of the double helical segments to form a three-dimensional network by complexation with cations and hydrogen

Preparation of ketoconazole loaded Proliposomes Ketoconazole (KTZ) loaded proliposomal gel formulations were formulated by method reported by Perret et al. 1991 with slight modification. Constant amount of drug was added to varying ratios of phophatidylcholine and cholesterol which constitute lipid component of 1mmol quantity. This lipid mixture was prepared in clean and dry, wide mouthed glass vials to which 400µL of absolute alcohol was added and after confirming the formation of homogenous dispersion

affinity of the component towards the stationary phase. In general, the more polar component would have a stronger interaction with the stationary phase, and the less polar component would be eluted out first. From the observations of the column chromatography, yellow S2 collected is β-carotene, indicating that

Thin Layer Chromatography (TLC) Abstract This experiment uses the TLC chromatography technique to identify the presence of acetylsalicylic and Acetaminophen in analgesic drugs (Tylenol and Anacin). It was found that the Anacin and acetylsalicylic had very closer Rf values (0.8 and 0.79). The Tylenol and acetaminophen had closer Rf values (0.54 and 0.58). Hence, Acetylsalicylic acid and acetaminophen were present in Anacin and Tylenol tablets respectively. Introduction Chromatography is the technique

COMPARISON OF INTRAVENOUS DEXMEDETOMIDINE VERSUS ESMOLOL FOR ATTENUATION OF HEMODYNAMIC RESPONSE TO LARYNGOSCOPY AND ENDOTRACHEAL INTUBATION Abstract: Background: Esmolol has an established role in attenuation of hemodynamic response to laryngoscopy and endotracheal intubation. We studied the effect of Dexmedetomidine compared to that of esmolol in this study. Aim: To study the role of dexmedetomine in attenuation of hemodynamic response to laryngoscopy and oral endotracheal intubation

Liquid chromatography is first being discovered by a Russian botanist, Mikhail Tsvet. Tsvet had rose the idea of liquid chromatography when he tried to purify and separate the coloured plant pigments by using a liquid-adsorption column containing calcium carbonate in 1890s. He also applied his observations with filter paper extraction to the new techniques for analysis the components in the petroleum. The filter paper extraction is the precursor of paper chromatography. He also found that the polarities

CHAPTER III BIGINELLI REACTION INTRODUCTION Dihydropyrimidinones (DHPMs), commonly known as Biginelli compounds, have attained unprecedented attention due to its greater biological, pharmaceutical and therapeutic properties. In 1893, Pietro Biginelli reported the first synthesis of 3,4-dihydroprimidin-2(1H)ones (DHPM) by a very simple multi-component one-pot condensation reaction of an aromatic aldehyde, urea and ethyl acetoacetate in ethanolic solution1 (Scheme 1.1). This efficient approach to

As previously stated, the following are the calculated RF values for each plate, Plate 1: Acetaminophen; Acetaminophen (Isolated)- 4.0/6.9= 0.57, Acetaminophen (Pure)- 3.8/6.9=0.55, Excedrin- 4.2/6.9=0.61 Plate 2: Caffeine; Caffeine (Isolated)- 1.9/6.8=0.28, Caffeine (Pure)- 0.8/6.8=0.12, Excedrin-4.0/6.8=0.59 and Plate 3: Aspirin; Aspirin (Isolated)-6.2/7.5=0.83, Aspirin (Pure)-6.2/7.5=0.83, Excedrin-6.8/7.5=0.9. The yield of each isolated analgesic was not calculated. The Rf values of the isolated

Electrophoresis in 1950. To separate molecules an electric current is applied to a gel containing biological specimen. Mixtures are placed in wells in the gel. When a current is applied molecules move and split corresponding to the molecules shape, charge, and size. Smaller molecules will move faster through the gel,

chromatography is meant those process which allow the resolution of mixtures by effecting separation of some or all their components in concentrated zone on or in phase different from those in which they are originally present , irrespective of the nature of the force or forces causing the substances to remove from one phase to another . ’’ (Williams et al, 1952) In this chromatography consists in applying a small drop of solution that is containing the substances to be separated to a strip of filter

1 Chromatographic decontamination 1) ion trade Decontamination of steed Ig by particle trade strategies has been portrayed. These creators took after the technique for Ter Avest et.al. (1992) with minor adjustments, utilizing DE-52 cellulose or DEAE CL-6b. 1gram DE-52 cellulose in 6ml 0.01m phosphate cradle (PB) ph6.0 was included every ml of serum. The DEAE CL-6b gel was washed twice with 0.5m Hcl, twice with 0.5m Naoh and twice with PB ph6.0 before utilization. For DEAE 1ml gel every 1ml serum

19.386526 -67.45 -44.1 20.53525 -68.39 -44.1 21.75204 -68.56 -44.1 23.04093 -67.97 -44.1 24.406191 -67.25 -44.1 25.852348 -66.75 -44.1 27.384196 -66.66 -44.1 29.006812 -66.79 -44.1 11.54782 -67.25 -44.1 12.232071 -66.3 -44.1 12.956867 -65.38 -44.1 13.72461 -64.56 -44.1 14.537844 -64.01 Adrian Bersiks_bersik_Acoustic Analysis_Excel.xlsx-44.1 15.399265 -63.86 -44.1 From the figure above there are no interpolation points above the reference line, which means the frequencies were bounded nicely

44 Several technique such as HPLC, supercritical fluid chromatography (SFC), and GC are used for separation of chiral compound. As a complimentary technique, capillary electrophoresis is also used for enantiomer separation.45 This technique has the advantage of high efficiency due to the plug-like flow profile which is created by the electroosmotic flow (EOF) and the low solvent and selector consumption.46 In CE, electrokinetic chromatography (EKC) is mostly used of chiral separation. In this technique

Leah Romero 10/30/2017 Conclusion Lab 3 Chem 102L In lab 3, fundamentals of chromatography, the purpose was to examine how components of mixtures can be separated by taking advantage of different in physical properties. A huge process in this lab was paper chromatography, which was used to isolate food dyes that are found in different drink mixes. The different chromatograms of FD&C dyes were compared to identify which dyes are present in each of the mixes. Chromatograms where made for the known

I. Attention: Imagine you have to go to McKinley because you accidentally ate spoiled food. You ask for penicillin, but doctor is unwilling to prescribe you the drug and tells you that you will recover after resting for a few days (AA). Most physicians refrain from prescribing drugs to patients because the overuse of drugs can reduce their effectiveness. Transition: However, farmers are ignoring this fact, and it is becoming a public health problem. II. Need: Farmers are giving an excessive amount