Lab Report Identifying Unknown Microorganisms
In this experiment, we cultivated an unknown specimen containing two microorganisms. The purpose of this experiment was to use a variety of biochemical test previously learned in the lab to identify the unknown bacteria. The identification of unknown bacteria is a major part of microbiology. Microbiologist observe samples such as blood and sputum in the laboratory for the presence of microorganisms. Identifying unknown bacteria is extremely important in clinical settings because it helps physicians find treatment for infections. I expect to learn the biochemical differences in bacteria from this lab. Also, how to identify different species of bacteria. Material & Methods For the first day of the practical, an unknown specimen was provided …show more content…
Mannitol Salt Agar: No growth occurred on this plate. Justification: Proteus vulgaris produces Hydrogen sulfide. The unknown organism produced Hydrogen sulfide in both the SIM Agar and TSI Agar. Proteus vulgaris is a motile microorganism that produces Indole. In the SIM Agar, the unknown microorganism was motile and produced Indole. Identification: Staphylococcus epidermis Justification: Staphylococcus epidermis is glucose fermenter. The unknown organism fermented glucose in the Phenol Red Glucose broth. Staphylococcus epidermis produces the enzyme catalase. In the PEA Agar, a catalase test was performed which showed that the organism produced catalase. Staphylococcus epidermis is not a mannitol fermenter. Mannitol fermenting organisms grow on the Mannitol Salt Agar. The unknown organism is not a mannitol fermenter because it did not grow on the Mannitol Salt Agar. Interpretation NA plate: The NA plate had growth of both organism even though it was difficult to differentiate between the colonies on this …show more content…
The organism fermented the sugar producing an acid which caused the phenol red pH indicator in the broth to become yellow. Phenol red is red in basic conditions and yellow in acidic conditions. Under oxygen limiting conditions, some organisms utilize sulfur compounds as terminal election acceptors (lab manual). The byproduct of this reaction is hydrogen sulfide. The medium contains iron salt which binds with hydrogen sulfide to produce a black color. The organism inoculated in this slant produced hydrogen sulfide because the butt of the slant was
Biofilms Lab Report
1999 Words | 8 PagesAs the strains used are the same as in the first test. A bio-film forming strain was identified in strain one and two from the initial experiment, and no biofilm forming strains were found in the third strain. This was reflected in this experiment. The first strain formed a black streak on the agar, representing the presence of a biofilm, which was expected from the previous experiment. Another expected result was the presence of the red streak in the red agar for the third strain suggesting no biofilm was present.
Gregor Mendel's Alleles: Garden Pea Plants
919 Words | 4 PagesGregor Mendel was born in 1822. He was an Augustinian monk and is known as the father of genetics, who carried out numerous crosses in the mid 1800s. He worked mainly on garden pea plants where his works led into two laws. Mendel’s law of segregation, also his first law states that, • Inherited characteristics are controlled by pairs of factors now known as alleles • These factors segregate at gamete formation so that only one factor is carried in each gamete.
Water And Sewage Microbiology Exam
445 Words | 2 PagesMicrobiology Unit 4 Exam Answer the questions fully, but do NOT more than is need to answer the questions (don’t write on and on and on….). Do Not COPY AND PASTE from any source. Please use your own words to answer. No plagiarism. You Must use Turnitin to submit the exam.
Bacterial Growth Lab Report
1360 Words | 6 PagesChapter 5 Bacterial Growth Micro-organisms such as bacteria can be grown in the laboratory if essential components required for their survival are present. The cat of growing bacteria in the lab is referred to as culture and the environment on to which the bacteria is grown is called culture medium. The essential ingredients required for bacterial growth include: o Carbon source o Nitrogen o Metal irons o Presence or absence of Oxygen o Optimal temperature
Did Pasteur's Help Arose With Fermentation Or Micro-Astronomer?
341 Words | 2 PagesWhile working with fermentation, Pasteur’s help was requested when problems arose with fermentation of beer. In his research (Berche 2012) “He observed the presence of small globules in the fermenting juice.” Those globes that Pasteur saw under a microscope turned out to be bacteria. He published his findings in 1857 and for the next several years, he continued his research using different mediums including milk, learning that the cause of food going bad was due to growing micro-organisms.
E. Coli Library Bacteria Report
1853 Words | 8 PagesMaterials and Methods Cloning E.coli Library Bacteria and Mysterious Bacteria The gradients of the media used for cloning E. coli bacteria included nutrient agar, ampicillin and arabinose sugar; X-gal was an extra gradient in unknown bacteria media. X-gal is an organic compound that consists of galactose linked to a substituted indole, it is used to detect β – galactosidase activity by yielding a blue compound. A purified E. coli library bacteria, containing a DNA fragment of the pG10 plasmid, was added directly on an agar plate and streaked using a sterile inoculation loop. According to the cloning protocol of unknown bacteria stock, 10 µL unknown bacteria was added to 90 µL nutrients media, and 10 – fold serial dilution were used to accurately
Kirby Bauer Diffusion Test Lab Report
624 Words | 3 PagesAseptic technique was initiated at the beginning of this experiment by cleaning the work surface with disinfected wipes. Personal protectives equipment was also worn. The material utilized in this experiment was: S. epidermidis culture broth, sterile cotton swab, streak plate, forceps in 70% alcohol, a lit tea light, and the three antibiotic disks (novobiocin, gentamicin, penicillin). The first step, I divided a plate into three quadrants and labelled them with the different antibiotic names. Using the lit tea light, like a bursen burner, I flamed the mouth of the S. epidermidis culture.
1 2-Propanediol Analysis
717 Words | 3 Pagesfinal major of their metabolism. MICROORGANISMS PRODUCING 1, 2-PROPANEDIOL Wide ranges of bacteria are acknowledged at present to ferment sugars for 1, 2-propanediol. The manufacture of this diol accounted in bacteria and yeast. Enebo as early as in 1954 reported Clostridium thermobutyricum to generate 1, 2-propanediol.
Vinegar Experiment
3183 Words | 13 PagesTITLE The production of vinegar by acetic acid bacteria the isolation of isolation of acetic acid bacteria from apple cider vinegar ABSTRACT The aim of the experiment was to produce vinegar, isolate and identify Acetetobacter sp from vinegar. It was to determine the concentration of acetic acid in vinegar.
E. Coli Bacteria Lab Report
617 Words | 3 PagesThe control plate, or -DNA, grew over three hundred bacteria colonies as there was nothing to stop its
Hand Washing Effectiveness Lab Report
718 Words | 3 PagesWashed each finger with the given regimen 3. Placed the washed samples on the corresponding quadrant of the agar plate near the center 4. Allowed the cultures to incubate at body 37 degrees C temperature for 7 days 5. Observations were made of each culture with the use of a dissection microscope to characterize the microorganism/colonies present in the agar plate.
Soil Phosphorus Research Paper
914 Words | 4 Pages(Richardson AE and Hadobas PA, 1997). Several species of microorganisms are able to secrete
Introduction To Crohn's Disease
2917 Words | 12 PagesSupervised by We certify that this thesis was prepared under our supervision at the College of Medicine, Al-Nahrain University as a partial requirement for degree of Philosophy of Doctorate in medical microbiology. In view of the available recommendation, forward this thesis for debate by the examining committee. Crohn’s disease results from a complex interaction between susceptibility genes, immune system and microbes that can trigger disease pathology. This study aims to determine the significance of ATG16L1 T300A genetic variant on host immunological status, clinical phenotype and presentation as well as the risk of microbial (Norovirus and/or ) acquisition
Bacterial Species
1566 Words | 7 PagesThis test along with the other IMViC tests is used to differentiate between the gram-negative species, similar to unknown 4. If the organism uses citrate as the primary carbon source, then they also contain ammonium phosphate as the nitrogen source. The medium used for this differential test is the Simmons Citrate Agar, which contains sodium citrate, and bromthymol blue is the pH indicator used to test for the presence of citrate. This test is performed with a light sample of the unknown bacterium, and a proper transfer should occur using aseptic technique between the unknown sample and the agar. If the organism presents growth or color change to blue, then this indicates a positive result for citrate as the carbon source such as in the species Enterobacter aerogenes.
Listeria Monocytogene Research Paper
700 Words | 3 PagesThe recent outbreak of food poisoning in South Africa that led to several deaths is Listeria(listeriosis). Listeriosis is an infection that is caused by consuming food contaminated by the bacteria Listeria. The infection can cause serious illness in pregnant women, neonates, adults with weakened or compromised immune systems and the elderly, and is capable of causing gastroenteritis in others who have been severely infected. Most people infected show no symptoms, but the elderly and very young are vulnerable. The outbreak would be discussed under the following Causative organism; Listeria monocytogene, is the organism that caused the outbreak.
More about Lab Report Identifying Unknown Microorganisms
Biofilms Lab Report
1999 Words | 8 PagesGregor Mendel's Alleles: Garden Pea Plants
919 Words | 4 PagesWater And Sewage Microbiology Exam
445 Words | 2 PagesBacterial Growth Lab Report
1360 Words | 6 PagesDid Pasteur's Help Arose With Fermentation Or Micro-Astronomer?
341 Words | 2 PagesE. Coli Library Bacteria Report
1853 Words | 8 PagesKirby Bauer Diffusion Test Lab Report
624 Words | 3 Pages1 2-Propanediol Analysis
717 Words | 3 PagesVinegar Experiment
3183 Words | 13 PagesE. Coli Bacteria Lab Report
617 Words | 3 PagesHand Washing Effectiveness Lab Report
718 Words | 3 PagesSoil Phosphorus Research Paper
914 Words | 4 PagesIntroduction To Crohn's Disease
2917 Words | 12 PagesBacterial Species
1566 Words | 7 PagesListeria Monocytogene Research Paper
700 Words | 3 Pages