Unknown Lab Report Unknown # 25 By: Jenna Riordan March 19, 2018 Bio 2843 1. Introduction Microbiology is the study of microorganisms found in all different environments throughout Earth, from the hot thermal vents at the bottom of the ocean to the ice at the top of a mountain. These microorganisms are used to teach us how multicellular organisms came to be and how they can survive today. These small, microscopic organisms are so unique that the identification of them is paramount in the advancements of science. Knowing the chemical makeup, the shape, and the biochemical processes is important in identifying these organisms to understand how they survive and where. A number of tests can be ran on an unknown bacteria to determine their ideal …show more content…
Crystal violet was then added for 60 seconds before being washed off with water. The mordant, Gram’s Iodine, was added for another 60 seconds before getting washed off with water. The heat fixed smear was then washed with 95% alcohol until the wash ran clear, leading to the final step of adding Safranin for 45 seconds before being rinsed with water. The slide was finally blot dyed with bibulous paper before it was placed under a microscope to observe the color and shape of the bacterium. 2.2 Litmus Milk Reaction A milk-based, litmus broth tube is incubated and observed after 48 hours. Observations include lactose fermentation without gas as well as with gas, the reduction of litmus, casein protein coagulation and casein and protein hydrolysis. These characteristics were all determined based on the color of the solution and the production of a curd, the curds density and the production of a gas. To determine the density of the curd, the tube was slightly turned to see rather or not it was mobile or concentrated towards the bottom. 2.3 Carbohydrate Fermentation of Lactose, Sucrose and …show more content…
The tube was placed back in incubation for 96 more hours to observe any more positives. 2.10 Catalase Test A trypticase soy agar plate was used and after incubation, four drops of 3% Hydrogen Peroxide was added to the plate to flow over the bacterial growth. A presence of bubbling was observed. 2.11 Starch Hydrolysis A starch agar plate was inoculated with a streak of the unknown bacteria and then incubated. On the second day of incubation, the plate was removed from the incubator and placed over a hot plate heating Iodine solids. The smoke of the Iodine stained the plate to display the presence or absence of a halo around the bacteria 2.12 Lipid Hydrolysis This test was done by making a single line streak inoculation on a tributyrin agar plate and allowing incubation. After the incubation period, the plate was observed for the presence or absence of a halo around the bacteria. 2.13 Gelatin Liquefaction A gelatin deep was deep stabbed and incubated. After incubation the tubes were placed in 4ºC for 30 minutes. The cultures were then examined to determine if gelatinase was solid or liquified 2.14 Oxidase
Being able to identify unknown microbes from systematic testing is what makes the field of microbiology so important, especially in infectious disease control. Using the testing procedure laid out by the microbiology field we are able to identify unknown bacteria present in our everyday lives, and along the way learn a lot about their characteristics that separate them from other types of bacteria. Being able to do this is vital in order for us to understand why microbes are present in certain places, how they are able to grow and what restricts their growth, that way they can be combatted if necessary. These techniques for determining unknowns are also important for isolating and testing infectious disease microbes in order to prevent spreading. Another important aspect of being able to identify unknown microbes is the
The unknown bacteria was then tested on multiple selective and differential media. Growth was present on the MacConkey Agar and the colonies were the same color as the plate, which told me my bacteria was gram negative and did not ferment lactose. There was no growth on the Mannitol Salt Agar, and this told me the unknown was not salt tolerant and did not
What information does this tell you about the bacteria in the sample, and why did the colony change colour? [2 marks] This tells me that the bacteria in the sample contains coliforms such as Escherichia coli (E.coli). The colonies changed colour as the enzymes produced by the bacterial cells reacted with the red galactosidase in the agar medium, this reaction causes the colonies to turn pink making them easily
ONPG produces the yellow color. The time was recorded when each tube turned yellow and incubated for 5 minutes in the water bath. 1ml of sodium carbonate (Na2CO3) is added to each to tube in order to develop the color as well as stop the reaction between ONPG and beta-galactosidase which will in turn stop the enzymes activity allowing us to measure the absorbance with no rush. The tube that turns yellow first is the one that has the highest levels of beta-galactosidase. It was hypothesized that the tube with the greatest amount of beta-galactosidase is produced in the tube that had the lactose mixture in the shaker for the longest time.
Starch solution is then placed into the test tube at a quantity of 5 mL. 5 drops of Lugol’s Iodine solution is added to the test tube. If the color changes, then it is known that starches are present in the solution. Proteins are next tested. In order to do this, 5 mL of gelatin solution is added to the test tube. 10 drops of Biuret’s reagent are added to test for protein.
On April 6, 2016 at approximately 11:45am, a local police station got a call about a hostage situation at a local pharmacy. When police and medical examiners got to each crime scene, they learned that all of the hostages were given drugs and had overdosed on them. Some of the pills, in powder form, were found near the victims. One of the victims was stable enough to tell the investigators that the power on the floor were the drugs they were forced to take. The medical examiner found out each hostage was given either unknown A or unknown B.
Exercise 14: Unknown Identification Lab Report The purpose of the study was to identify the unknown bacterium using various biochemical tests in addition to using scientific methods in determining the outcome of the hypothesis. Each biochemical test will help determine the bacteria based on specific characteristics of each organism. I was giving unknown number 232. The first procedure that needed to be done after obtaining unknown bacterial mixture was to isolate the two bacteria in a pure culture using the streak plate method described in Microbiology Laboratory Manual Eight Edition. The material used was trypticase soy agar (TSA) plate, nutrient plate, starch agar, hydrogen peroxide, iodine reagent and microscope.
The Another medium used was MAC, it is used to isolate and differentiate gram-negative organisms and it is a pink, dusty rose color. Lastly, the Citrate Slant is a green color and it was used as a differential test to examine enzymes. The media were inoculated at 37°C for 48 hours, then it was observed to determine the
The organism that was successfully cultured from the first lab was use to streak into other plates. Samples from the previously cultured MAC and blood agar were streaked on two different sides of an EMB, SS, and MSA plate. Samples of bacteria were also used to inoculate Citrate and TSI media. The inoculation of TSI and Citrate media were as follows: The materials were gathered, which include the previously cultured media, an inoculation needle and the Citrate and TSI medium.
INTRODUCTION: In this experiment I was testing for antimicrobial sensitivity of Staphylococcus epidermidis by using the Kirby-Bauer Diffusion test. The three antibiotics utilized in this lab were: gentamicin, novobiocin, and penicillin. I determined the effectiveness of the antibiotic by observing and measuring the zone of inhibition for each antibiotic.
Many bacteria from natural environments exist in a conglomerate and are difficult to isolate and cull from the other members of that partnership. Bacteria can be found as individual cells or in the large colonies shaped like tight coils, clusters, filaments or thin biofilms. The identification of bacteria in the laboratory is exceptionally relevant in medicine, where proper treatment is determined by isolating and classifying the bacterial species causing a given infection. The need to identify pathogens was also an impetus for advancement of clinical techniques used to identify microbes in
Uncontrolled Environmental conditions Atmospheric conditions The controlled variable Concentration of amylase was kept under control by measuring the amount of amylase used and also it was made sure the percentage of amylase used was 1%. The Amount of amylase/starch used were kept to 5cm3 at all times. Materials needed Beakers Bunsen burner Test tube Thermometer Stopwatch Test plate Glass rod Starch Amylase solution Water bath Iodine solution. Test tube holder Labels Marker Procedure First 5 test tubes were taken and labeled with numbers from 1 to
Nguyen Nguyen Professor Microbiology 1 May 18th, 2016 01MW – Staphylococcus Epidermidis The Staphylococcus Epidermidis is classified as bacteria. Scientists reckon it to Firmicutes phylum and adjust it in Bacillales order of Bacilli class. This bacteria belongs to Staphylococcaceae family.
Microbes were the first organism to occupy the Earth. They are a group of the smallest organisms and are single cellular organisms. Microbes are becoming important in areas such as biochemistry, genetics, agriculture and medicine. They are also important decomposers. One example, of a microbial life is the prokaryotes called bacteria.