Results Continued: The purpose of this experiment was to show the transformation of E. Coli with pGLO. We made four different plates each with different additives to compare them to one another, and be able to track the transformation. Initially we had predicted that only one of the four plates would glow. The plate with the plasmid (+pGLO)/LB/amp/ara was the one we said would grow and glow because it contained all the necessary tools to do so. The plate that grew and glowed included the plasmid with the green fluorescent protein (GFP), to make it glow. It also included the resistance gene to ampicillin, as well as the Luria Bertani broth to make the bacteria grow by feeding it, and lastly the arabinose which is in charge of turning on the …show more content…
The plate without the plasmid that contained ampicillin, and the LB broth did not grow because ampicillin is an antibiotic that impeded the growth of bacteria because it killed off. There was no glow because it didn’t contain the green fluorescent protein gene or any other of the components to make it glow. The plate that contained the +pGLO, the ampicillin, and the LB broth was able to grow, because the plasmid provided the ampicillin resistance gene and the LB broth to feed the bacteria, however it wasn't able to glow because no arabinose was present to activate the GFP gene. Another plate that was able to grow was the one without the plasmid and just the LB broth. Finally the plate with all the components, +pGLO, arabinose, ampicillin, and broth was able to both grow and glow because the arabinose (sugar) turned the GFP gene on, the resistance to ampicillin gene was present and that let the bacteria survive and feed on the LB broth which let the culture …show more content…
GMO's are genetically altered organisms made by scientists. By altering the organisms' genetics it could change its size, color, taste, and physical appearance. A GMO is meant to be stronger, better and simply more effective than past generations that haven't been altered. They are more capable of adapting and surviving new environments they wouldn't naturally be able to. For example crops can be made pest resistant, sweeter, bigger, be able to grow faster, be able to grow under any type of conditions rather than having to wait for certain season to get that certain type of crop and even be the new 'edible vaccines'(Scientific American Inc). However, GMO's are controversial because chemicals are used to alter the genetic information of a crop. Those chemicals can increase the risk for certain illnesses, such as diabetes and cancer. GMO's are meant to be the perfect organism, however many factors can go wrong with so much genetic modification since its going against nature (Food Policy Institute 2003). The process of how GMO's are created is through the help of gene transfer technology. The desired gene from another organism is placed in the other organisms genome and they remove the gene that is not desired, what we would call the faulty gene (University of Utah Health Sciences 2015). The University of Utah states that children who have not vaccinated could still develop the necessary
The purpose of this lab report is to employ a myriad of skills, tools and, methods learned throughout this semester to perform the appropriate tests for the identification of the assigned unknown bacteria. Add more background information here!!! The most important tools and techniques used during this identification include aseptic technique, microscopic examination and, the use of selective and differential media. Aseptic technique is an important tool for microbiologists. It is imperative that aseptic technique is maintained throughout the length of any test to avoid any cross-contamination that may lead to inaccurate results.
The Mut section is expected to have no growth as mutants require the amino acids leucine and valine to grow which is not provided in the minimal medium. Results Figure 2. Testing of mutant mixed with DNA, mutant bacteria and DNA on LB medium Growth was observed on the Transformed (Trsf) section and the Mutant (Mut) section but not on the DNA section. Due to human errors, the photo of our experiment was lost, but we have obtained similar results as from group1.1 and their photo is presented.
Unknown Lab Report Unknown # 25 By: Jenna Riordan March 19, 2018 Bio 2843 1. Introduction Microbiology is the study of microorganisms found in all different environments throughout Earth, from the hot thermal vents at the bottom of the ocean to the ice at the top of a mountain.
But, since no arabinose was present, the bacteria was unable to glow. In the second dish, +pGLO LB/amp/ara, the bacteria was able to glow and grow since there was the pGLO plasmid which contains the gene that is ampicillin resistant, and the sugar arabinose which activates the araC gene which allows the bacteria to glow. In the third dish, -pGLO LB/amp, the bacteria did not glow or
Introduction Our world is composed of many bacteria’s’ that can either help or destroy us. Therefore, its’s imperative to learn and study them. The purpose of the lab was to put into action the methods that have been learned in the laboratory to determine our unknown bacteria. Bacteria’s can have different features, shapes, and or arrangements that help microbiologist determined their role in our life (whether they are good or bad for humans).
The purpose of this lab was to perform a procedure known as genetic transformation which allowed us to genetically engineer E. Coli to be ampicillin resistance. Before the lab we expected that lysogeny broth and minus DNA will have growth but no glow. The lysogeny broth, ampicillin, and
Bethany Brookshire, the author of the article “New gene resists our last-ditch drug” found in the Society for Science & the Public, invoked fear and urgency in teen readers fascinated with biology and health. Throughout her article, Brookshire establishes that doctors, farmers, and everyday citizens should be cautious in the use of antibiotics and use methods to limit the spread of harmful bacteria worldwide. She gains her readers’ attention and trust by quoting information from several scientists in different fields and from different parts of the world. Although her syntax was rigid and overly simplified, Brookshire connect to the teen readers ****** Brookshire is professional and *** in her popular article. She maintains an unbiased standpoint
Hypothesis The transformed E.Coli with the ampicillin resistance gene will be able to grow in the ampicillin plates and it would have a green glowing color.
The second strain formed a dark red streak, however, which was unexpected as a dark streak indicating a biofilm was expected from the initial experiment. However, the dark red streak indicated some biofilm was present, although not as much as in the first
Exercise 14: Unknown Identification Lab Report The purpose of the study was to identify the unknown bacterium using various biochemical tests in addition to using scientific methods in determining the outcome of the hypothesis. Each biochemical test will help determine the bacteria based on specific characteristics of each organism. I was giving unknown number 232. The first procedure that needed to be done after obtaining unknown bacterial mixture was to isolate the two bacteria in a pure culture using the streak plate method described in Microbiology Laboratory Manual Eight Edition. The material used was trypticase soy agar (TSA) plate, nutrient plate, starch agar, hydrogen peroxide, iodine reagent and microscope.
The plasmid and the gene are both cut using restriction enzymes. To incorporate the gene into the plasmid, both the plasmid and gen stuck together by a bacterial enzyme called ligase. The plasmid with the foreign gene can then be inserted into the bacteria One specific plasmid is pUB110 which is circular whose host is Bacillus subtilis. It has a plasmid size 2.3 kpb and copy number between 20 - 50. We can selectively grow bacteria that contain this plasmid by using selective and differential media.
Carefully remove the comb and tape surrounding the plate and transfer the gel plate to the electrophoretic tank such that wells are towards the cathode 4. Pour 1X TAE buffer into the tank until the gel is completely submerged. Connect the electrode to the power supply 5. Load the plasmid DNA preparation and standard DNA marker into separate well with help of a micropipette or a syringe 6. Turn ‘ON’ the power supply and run at 100 V (10-15 mA).
They also are way way better than non gmo foods because non gmo foods you can get diseases from them like heart disease and cancer and the chance of having a stroke when you eat though’s foods, But when you eat foods that have gmos in the foods then the risk of having them diseases. If you buy gmo foods you won’t have to spend a lot of money on the food that you would need but if you buy non gmo foods then you would spend a lot of money just on the food that you would need to
Nguyen Nguyen Professor Microbiology 1 May 18th, 2016 01MW – Staphylococcus Epidermidis The Staphylococcus Epidermidis is classified as bacteria. Scientists reckon it to Firmicutes phylum and adjust it in Bacillales order of Bacilli class. This bacteria belongs to Staphylococcaceae family.
The creation of the master plate was made up of specially selected bacteria from both experiment 3.2 and 4.1, the goal was to achieve a diverse selection of bacteria to observe closely at its texture, color, shaper, and margins. When selecting specific bacteria, I looked for the best combination of variation and choosing across all five of my plates for both agars. Through using morphological observation I was able to determine different bacteria such as colonies that are irregular and circular continuing with variations of elevations and edges. Also, the use of solid cultures such as the use of agar is rewarding because the bacteria will not move creating the ability to have many colonies on the plate while still being isolated and effectively