Ninhydrin Test Report
Amino acids are known as the building blocks of all proteins that consists of 20 amino acids which are found in within proteins convey a vast array of chemical versatility. Amino acids are comprised of a carboxyl group and an amino group that attached to the same carbon atom which is the α carbon. They vary in size, structure, electric charge and solubility in water because of the variation in their side chains (R groups). Detection, quantification and identification of amino acids in any sample constitute important steps in the study of proteins. An amino acid that bonded directly to the alpha-carbon have an amino group is called alpha amino acids. Figure 1: Structure of alpha amino acid (http://www.aminoacidsguide.com/)
Ninhydrin test
…show more content…
This diluted solution will be used in the assay as duplicate samples. Then, 1.0mL of standard glycine solution containing (7.5mg/mL) was diluted to 100mL with water using a volumetric flask. This solution contains 1.0µmole/mL of glycine. 8 tubes were set up according to the following protocol and 2.0mL of ninhydrin reagent was then added to each of the 8 tubes and were placed in a boiling water bath for 20 minutes. After 20 minutes, the tubes from the bath was carefully removed, cooled in a beaker of cold water, then 8.0mL of 50% ethanol was added and mixed well. The absorbance of each tube was determined at 570nm and the absorbance were plotted against amount of standard glycine.
For determination of tyrosine content using Folin & Ciocalteu’s reagent, seven tubes were set up. Then, 1.6mL of 0.5M Na2CO3 was added to each of the tubes and were mixed thoroughly. 0.4mL of the Folin & Ciocalteu’s reagent then were pipette in, mixed thoroughly, and allowed to stand for 10 minutes. The absorbance of each tubes was determined at 660nm and the absorbance were plotted against amount of standard
Unknown Microbial # 398
532 Words | 3 PagesLast test, inoculation of phenylalanine agar is used to determine if phenylalanine deaminase oxidizes phenylalanine into phenylpyruvic acid and ammonia. Sixth test, is a Multiple Test Media used to determine the physiological characteristics of unknown #398. First test, Inoculation of Kligler 's Iron agar was used to determine the production of hydrogen sulfide from cysteine and fermentation of glucose and lactose. Last test, inoculation of litmus milk is used to determine the fermentation of lactose, casein, lactalbumin, and
Nt1210 Lab Report
798 Words | 4 PagesResults The lab experiment was done in two parts, one with the NAND, NOR, XOR and Hex Inverters and the other with a 7483 full adder gate, both will verify the truth table when two input bits and a carry are added together. The circuits were built by examining the 1 bits through a K-Map to create a Boolean expression for the sum and carry. The Boolean expression for the sum was A⊕B⊕C and the carry as AB+BC_in+AC_in. From these two expressions, we notice that we must use two exclusive-ORs gates in the sum inputs for A, B, and C. For the sum, we have to use NOR and NAND (the only available gates from the lab manual).
Nt1310 Unit 10 Lab Report
302 Words | 2 Pages%% Init % clear all; close all; Fs = 4e3; Time = 40; NumSamp = Time * Fs; load Hd; x1 = 3.5*ecg(2700). ' ; % gen synth ECG signal y1 = sgolayfilt(kron(ones(1,ceil(NumSamp/2700)+1),x1),0,21); % repeat for NumSamp length and smooth n = 1:Time*Fs '; del = round(2700*rand(1)); % pick a random offset mhb = y1(n + del) '; %construct the ecg signal from some offset t = 1/
Nt1310 Unit 4 Lab Report
603 Words | 3 PagesSuppose we have a single-hop RCS where there is one AF relay that amplifies the signal received from a transmitter and forwards it to a receiver. Assume that the transmitter sends over the transmitter-to-relay channel a data symbol ${s_k}$, from a set of finite modulation alphabet, $S={S_1, S_2,ldots,S_{cal A}}$, where ${cal A}$ denotes the size of the modulation alphabet. The discrete-time baseband equivalent signal received by the relay, $z_k$, at time $k$ is given by egin{equation} z_k = h_{1,k}s_k + n_{1,k},~~~~for~~k=1,2,ldots,M label{relaySignal} end{equation} where $n_{1,k}sim {cal N}_c(0,sigma_{n1}^2)$ is a circularly-symmetric complex Gaussian noise added by the transmitter-to-relay channel, $h_{1,k}$ denotes the transmitter-to-relay channel, and
Nt1330 Unit 6 Lab Report
74 Words | 1 Pages1. What area/aspect of this setting is the most challenging? 2. In the setting, you work in, is there a certain population of patients you see more? How does this affect you?
Mitochondria Lab Report
737 Words | 3 PagesAlthough the overall absorbance increases as more milliliters of mitochondrial suspension is added to a mixture of 0.25 mL of 0.5 mM DCIP, 0.5 mL of 50 mM sodium azide, various volumes of assay buffer (20 mM potassium
Quetiapine Fumarate Case Study
3223 Words | 13 PagesThe mobile phase used was a mixture of ammonium acetate buffer and acetonitrile at a ratio of 400:600. A flow rate of 1 mL/min was maintained, and the detection wavelength was 292 nm (22). The required studies were carried out to estimate the precision and accuracy of the HPLC method and were found to be within limits [percent coefficient of variation was less than 15%]. Sample preparation briefly involved 0.4 μ membrane filter through which the sample was filtered, diluted with mobile phase, and 10 μL was spiked into
Presence Of Macromolecules In Mcdonald's Happy Meals
1184 Words | 5 PagesStarch solution is then placed into the test tube at a quantity of 5 mL. 5 drops of Lugol’s Iodine solution is added to the test tube. If the color changes, then it is known that starches are present in the solution. Proteins are next tested. In order to do this, 5 mL of gelatin solution is added to the test tube. 10 drops of Biuret’s reagent are added to test for protein.
Nt1310 Unit 5 Lab Report
265 Words | 2 Pages\section{Facility Static and Dynamic Control}\label{Calibr} The facility calibration is the transfer function between the oscillating gauge pressure $P_C(t)$ in the chamber (described in ~\autoref{Sub31}) and the liquid flow rate $q(t)$ in the distributing channel, i.e. the test section. Due to practical difficulties in measuring $q(t)$ within the thin channel, and being the flow laminar, this transfer function was derived analytically and validated numerically as reported in ~\autoref{Sub32} and ~\autoref{Sub33}. \subsection{Pressure Chamber Response}\label{Sub31} Fig.\ref{fig:2a} shows three example of pressure signals $P_C(t)$, measured in the pneumatic chamber.
Nt1310 Unit 5 Lab Report
259 Words | 2 PagesCt ε0A (9)where ε0is the permittivity of the free space, C is the capacitance, t isthe thickness of the sample and A is the area of the cross section. Thedielectric constant has high values in the lower frequency regionand then it decreases with the applied log frequency as shown inFig. 10(a). The plot of the dielectric loss vs.
Nt1310 Unit 6 Lab Report
1151 Words | 5 PagesSo these would tend to have more reflections building up, to see that the biggest buildup is in the 400Hz band gives an idea how these materials can influence each other to resonate at a lower range than the previous
Amino ACID-Build Muscles Effectively With BCAA And EAA
1012 Words | 5 PagesIn practice we talk also about branched chain amino acids or BCAAs (Branch Chained Amino Acids). This group includes isoleucine, leucine and valine. These amino acids are essential and they contribute to several important functions in the body. They include a significant role in our stress reactions and the circulation of energy and muscles.
Deoxyribose Lab
2057 Words | 9 PagesThe reagents used were Diphenylamine reagent which contains concentrated H2SO4. The standard solution used for this test is the deoxyribose standard solution. In the sample, only a faint blue solution appeared, which indicates a small presence of deoxyribose. In test for Phosphate, the standard solution was the Phosphate solution and the reagents used were concentrated H2SO4, concentrated HNO3, 2.5% ammonium molybdate solution.
Organ Bath Experiment
1445 Words | 6 PagesNext, a basic stock solution was used to prepare various concentrations ranging from 1.0 x 10-8M to 1.0 x 10-1M by serial dilution. The tissue was washed by overflow with reservoir’s solution for 5 seconds to obtain baseline before adding 0.1ml, 0.3ml and 0.5ml for each concentration respectively into the tissue bath. The tissue’s peak response for each final bath concentration(FBC) was measured and recorded. Rmax and EC50 of histamine were recorded.
Arginase Lab Report
1437 Words | 6 PagesThroughout the urea cycle, the amino acid, arginine, is changes into ornithine- this is another amino acid when hydrated, that is when water was added. During this reaction, urea is the product formed (Nelson and Cox 2008). Figure 1 shows the urea cycle, occurs specifically in the mitochondria and cytosol in the liver. (Nelson and M.Cox 2008). Urea is made in the liver by means of enzymes in the urea cycle.
More about Ninhydrin Test Report
Unknown Microbial # 398
532 Words | 3 PagesNt1210 Lab Report
798 Words | 4 PagesNt1310 Unit 10 Lab Report
302 Words | 2 PagesNt1310 Unit 4 Lab Report
603 Words | 3 PagesNt1330 Unit 6 Lab Report
74 Words | 1 PagesMitochondria Lab Report
737 Words | 3 PagesQuetiapine Fumarate Case Study
3223 Words | 13 PagesPresence Of Macromolecules In Mcdonald's Happy Meals
1184 Words | 5 PagesNt1310 Unit 5 Lab Report
265 Words | 2 PagesNt1310 Unit 5 Lab Report
259 Words | 2 PagesNt1310 Unit 6 Lab Report
1151 Words | 5 PagesAmino ACID-Build Muscles Effectively With BCAA And EAA
1012 Words | 5 PagesDeoxyribose Lab
2057 Words | 9 PagesOrgan Bath Experiment
1445 Words | 6 PagesArginase Lab Report
1437 Words | 6 Pages