Lactose is a disaccharide, a sugar that is formed when glucose and galactose, simpler sugars or monosaccharides undergo a condensation reaction. This disaccharide is found in the milk of mammals and provides a source of energy for infants that rely on their mother’s milk and acts as a substitute to solid foods and provides nutrition. Lactose is digested, absorbed by the small through the help of the enzyme lactase. This is imperative as it cannot be absorbed in its plain form and so must be broken down into simpler sugars. This reaction is called a hydrolysis reaction in which water that is formed as a by-product in a condensation reaction is used to breakdown the covalent bond in the disaccharide, known as the glycosidic linkage, to form two
Web. 26 Oct. 2015.www.study.com Disaccharides, or sometimes called double sugar, is composed of two molecules of simple monosaccharides connected to each other. Disaccharides are crystalline water-soluble compounds. The monosaccharides within molecules are connected by a bond called, glycosidic. The three main disaccharides are lactose, sucrose, and maltose.
A milk-based, litmus broth tube is incubated and observed after 48 hours. Observations include lactose fermentation without gas as well as with gas, the reduction of litmus, casein protein coagulation and casein and protein hydrolysis. These characteristics were all determined based on the color of the solution and the production of a curd, the curds density and the production of a gas. To determine the density of the curd, the tube was slightly turned to see rather or not it was mobile or concentrated towards the bottom. 2.3 Carbohydrate Fermentation of Lactose, Sucrose and
ST Report In the experiment, the problem was the contaminants that were affecting the quality of the water samples. To fix this issue, three scientists had to determine the contaminants that were present in the samples. One sample was from the school sink and the second sample was from an unknown source. The scientists conducted many tests to figure out what pollutants were present in the water.
The human body consists of enzymes which allow foods to be broken down and enable organisms to build chemical substances such as other proteins, carbohydrates and fats that are necessary for life. This experiment will be testing the enzymatic reactions with toothpicks. This experiment depicts the process when a substrate binds with an enzyme. However, only a specific substrate can attach to a specific enzyme. This allows the substrate to attach to the enzyme and then disconnect the enzyme overall causing the substrate to break in two.
The substrates bind to a region on the enzyme called the active site. The active site is precisely shaped to hold specific substrates. Beta-galactosidase is one of the three genes in the lac operon. A lac operon is an operon required for the digestion of lactose in bacteria cells. B-galactosidase converts lactose, a disaccharide, into glucose and galactose, monosaccharides.
C12H22O11 (lactose) + H2O > 4CH3CHOHCOOH (lactic acid) is the equation for lactose to lactic acid.
B-galactosidase breaks down the disaccharide lactose into simple sugars glucose and galactose. However, glucose is a colorless compound hence it has to be substituted with a compound that is detectable by a visible color change. Hence,
For lactose to be absorbed, it must first be split into two simple sugars, glucose and galactose. The glucose and galactose are then absorbed by the cells lining the small intestine. Lactose intolerance is caused by reduced or absent activity of lactase that prevents the splitting of lactose (Marks). The breakdown of milk into its component building blocks allows it to be readily absorbed into our blood streams so its calories from sugars, lactose, proteins, casein, fats, vitamins, and minerals can be used efficiently.
One of the largest class of last resort clinically used antibiotics worldwide are β-Lactams, such as cephalosporins and carbapenems. β-Lactams inhibit the transpeptidase enzymes which aids in cross linking adjacent peptidoglycans strand. This inhibits the cell wall synthesis which then causes the cell membrane to lysis and causes cell death. They are valued because of their broad activity, limited toxicity and the bioavailability. Unfortunately, bacteria have developed a resistance to the β-Lactams by the expression of the enzyme called β-lactamases, which clinically inactivates the antibiotic by hydrolyzing the four-membered lactam ring of the β-Lactams.
Blood Glucose Response to Consumption of Beverages Containing Carbohydrates Following a 30 Minute Bout of Moderate Intensity Exercise Michael Griffith Comprehension: Carbohydrates (CHO) can be found in anything from breads and pastas to fruits, candy bars and even various beverages. These molecules are of utmost importance for the human body due to the fact that they are its main source of energy during rest, as well as exercise. A lack of CHO decreases physical performance and can inhibit post-exercise recovery (4). Proper CHO levels help prevent gluconeogenesis, improve recovery and have been suggested to improve performance and decision making in sports like soccer, tennis, and basketball (2).
Joshua Miller 12/18/17 Fermentation Lab report Introduction The term fermentation refers to the chemical breakdown of a substance by bacteria, yeasts, or other microorganisms, typically involving effervescence and the giving off of heat (wikipedia). Sugars are converted to ethyl alcohol when fermentation happens. In this experiment we determined if yeast cells undergo fermentation when placed in a closed flask with no oxygen. Glucose and yeast are mixed together in a closed flask and allowed to incubate for about one hour.
AN INTRODUCTION TO THE VARIOUS CARBONATED SOFT DRINKS AVAILABLE IN THE MARKET Soft drinks, especially carbonated soft drinks are products of western culture. The first known instance of manufacture of carbonated soft drinks was in the year 1767 when the British scientist Joseph Priestley discovered a system of mixing carbon dioxide in water to prepare carbonated water. His invention was known as soda water and this invention was the founding stone for the proliferation of carbonated soft drinks in the world. A Swiss person, Johann H Schweppe founded the Schweppes company in the year 1783 in Geneva for selling carbonated water.
Biochemical tests are the tests used for the identification of bacterial species based on the differences in the biochemical activities of different bacteria. Bacterial physiology differs from one species to the other. These differences in carbohydrate metabolism, protein metabolism, fat metabolism, production of certain enzymes and ability to utilize a particular compound help them to be identified by the biochemical tests. Gram’s stain was originally devised by histologist Hans Christian Gram in 1884. Gram-positive bacteria stain purple, while Gram-negative bacteria stain pink when subjected to Gram staining.
Using glucose would require lower temperatures and reach a much higher solubility faster. Due to the fact that the lactose and sugar need higher temperatures to reach a higher solubility, these solutes would most likely create supersaturated solutions. This is because the solution would need more heat in order to dissolve the
Galactosemia is a type of genetic disorder that modifies the process of breaking down galactose, a simple sugar. Galactose is essentially part of a common sugar named lactose. Furthermore, it can also be present in a variety of foods, especially in baby formulas and dairy products. “Galactosemia” is a term that represents that the blood contains too much galactose.