Daphnia Experiment

The hands were washed. Goggles were placed on the eyes for extra protection. A petri dish containing nine pill bugs were obtained and set aside for later use. Two water bottles’ ends were cut and taped to each other creating a miniature tunnel for the pill bugs to crawl in. 50 mL of tap water was obtained in a small beaker and then was slowly moved into the lid of the bottle using a funnel. The sugar used in this experiment was created by mixing ten milliliters of starch,glucose, and regular countertop sugar. This was transferred into the joint water bottle tunnel using a funnel. Both ends of the tunnel were sealed shut and each measurements were taken every three minutes and final measurements were taken after 21 minutes. The results were

Introduction- Isopods, or what your 7-year old self would refer to them as ‘Roly-Polys’, are actually a type of class crustacean. They breathe with gills, so they have to stay in damp areas with high humidity. That’s why you normally find them under rocks (Isopod, Pillbug, Sow Bug Information, 1997). Also, they like to eat Detritus and it is a more acidic plant (Animal Behavior: Pillbugs and Their Preferences, 2003-2015). This being said, would isopods refer to be in an area with low pH, high pH or water? I there is a low pH, high pH, or water, then the isopods would prefer the water because most organisms are sensitive to certain pH levels.

Daphnia Magna are small crustaceans that are excellent for observing the effect of depressants on their nervous system. The purpose of this specific experiment is to observe the effect of ethanol, aspirin, and caffeine on daphnia’s heart rate. Not only do these chemicals affect heart rate, but they also affect the frequency of reproduction, number of eggs produced, and body structure. Pesticides similar to these chemicals can be released into the environment and daphnia re useful in monitoring toxicity levels. The agents being tested in this experiment can be administered to determine the effects of hormones, stimulants, antidepressants, and neurotransmitters. The hypothesis for this experiment is that if daphnia are exposed to ethanol, aspirin,

Daphnia play a vital role in the ecosystem because they are an important part of the aquatic food web (Miner et al., 2012). When exposed to UV light, Daphnia swim deeper into the water (Morris et al., 1995). This natural movement is called phototaxis.

50ml of the water was obtained and placed into a Styrofoam cup. A capsule was then added to the water and a timer was started. A stirrer thermometer was used to stir the water at a constant pace until the sponge emerged. Once the sponge creature emerged the timer was stopped and the time recorded. This experiment was repeated once more with distilled water heated to 80̊C (1). Once the results from each group were documented an unpaired t test was preformed using the collected data. The t test results were then plotted and graphed to determine whether or not heat had an effect on the emergence time of the sponge creature from the gelatin

After performing the Brine Shrimp Inquiry lab my group found that .5% salinity of 50 mL of water was the ideal salinity for hatching brine shrimp. To figure out what salinity was ideal we tested three different levels of salinity. The first thing we did when we began the lab was choose three different salinities to test. My group choose .5%, 3%, and 5%, next we choose the amount of water that would be in each dish; we decided on 50 mL of water. We then calculated the amount of salt to put in each dish. To do this we created proportions. We calculated that we need to put .25 mL of salt in 50 mL of water to create .5% salinity, 1.5 mL of salt in 50 mL of water to create 3% salinity, and 2.5 mL of salt

For two days, on the 14th and 15th of April, a field excursion to Hastings Point, New South Wales was conducted. At Hastings Point, topography, abiotic factors and organism distribution were measured and recorded, with the aim of drawing links between the abiotic factors of two ecosystems (rocky shore and sand dunes), the organisms which live in them, and the adaptations they have developed to cope with these conditions. Within these two ecosystems, multiple zones were identified and recorded, and this report also aims to identify the factors and organisms associated with each zone. Lastly, using data and observations from the past, predictions for the future of the rock pool ecosystem were made.

It can cause the increase in heart rate which can lead to arrhythmia, heart palpitation or heart attack.

Nonetheless, besides fish, many other kinds of poikilothermic animals, such as snakes and frogs, use lungs instead of gills to breathe, and further researches may be conducted to explore if caffeine has an effect on their metabolism. For example, forty northern leopard frogs with similar body size will be captured and divided into twenty trials, and each two frogs will be tested in the same control and experimental conditions as the goldfish metabolism experiment did. The collected slopes of oxygen concentration will then be converted into oxygen consumption rate and analyzed by the paired t-test. If the difference in the oxygen consumption rate between the control and experimental groups is statistically significant, this experiment will show that caffeine affects the metabolism of frogs, one kind of poikilotherms that use lungs to

Using the 0.1 M stock solutions of sugar, a 0.01 M dilution was created for each sugar type by adding water to the stock solution 9 out of 1. A 20 mL dilution was made for each trial. The same volume of each solution (10 mL -5 mL ) was added to the green sponges to create four group; Group 1: Sponge with 10 Ml 0.01 M glucose solution, Group 2: Sponge with 10 mL 0.01 M fructose solution, Group 3: Sponge with 10mL 0.01 M sucrose solution, Group 4 (Control): Sponge with 10 mL water. The four sponges were placed every 90 degrees on the edge of the arena. The isopods were placed into their environment for one to two minutes to acclimate with the environment. Once acclimated, the isopods walked around the arena for five minutes and go to the sponges that they preferred. The experiments should be repeated 3 times with the same design layout for accurate results. 20 isopods were released in the center of the arena. After five minutes, the number of isopods behind every sponge was

Although there are differences in coffee and energy drinks there are also similarities between the two. One fact is coffee and energy drinks help with concentration and physical endurance performance. These drinks are both addictive. They both can increase your heart rate. Another similarity, is that their popular drinks among the people of the world. whenever you drink too much of either of them, it may hinder your health if taken in of abnormal quantities and cause an overdose if taken with other caffeine products. Taking precautionary measures is an important thing when consuming many of these caffeinated drinks. Having coffee or

Dropping warm Ringers solution on the frog’s heart would increase the heart rate because the calcium channels open faster.

Antagonistic behaviors are those conflict behaviors that include aggressive and submissive behaviors. Antagonistic behaviors tend to occur over territoriality. Every animal is looking for the most advantageous place to live and this results in having conflicts with other animals similar to their species and their needs. Animals fight for territory for many reason including: competition for food, to avoid interruptions while mating, survival and others. Fighting becomes a result from intrusion. Many animals mark their territories in different ways. The final result of the fight tends to favor an individual in a higher social position over the other (Wilson, 1975). Meaning, at the end of the fight, one

The purpose of this article was to test the effects of chemicals on Daphnia magna. Caffeine, Nicotine, ethanol, and a control solution were tested. The heart rate of Daphnia was measured after exposure to each of the chemicals. After testing, it was found that ethanol depressed the heart rate while exposed, but the heart rate stabilized after exposure. With exposure to nicotine, the heart rate increased by 20%. If more nicotine was added, the effects were varied and sometimes irreversible. Caffeine did not have a measurable effect on the heart rate

The topic of this research paper will cover over Ocean Acidification and the effects on marine ecosystems. Ocean acidification is the decrease in pH levels of the Earth 's oceans, due to overbearing carbon dioxide being brought by the atmosphere. The carbon dioxide is drawn by seawater, where the chemical reactions take place, which minimize the pH levels in the ocean, the carbonate levels and the important calcium carbonate which these sea creatures need. When carbon dioxide disperses in this ocean, it causes the levels of acidity to rise towards the the surface, where most shell growth in marine animals have been stopped and has created the cause of reproductive disorders in multiple fish. Part of the problem with ocean acidification is human activity. Ever since the industrial revolution commenced, around one third of the carbon dioxide released by human interference has been absorbed by the ocean, which is one of the reasons of the drastic change in climate as well. Without the ocean absorbing carbon dioxide, the carbon dioxide n the atmosphere would be drastically higher, with possibly increasing the levels of climate change.The point of the research is to see how will the pH affect the organisms in the marine ecosystem and does carbonation affect an organism’s mass and form. Many organisms that are part of the marine food chain are going to be affected by ocean acidification due the levels of acidity deteriorating the calcium carbonate within the animal. It turns out