The sample was transferred to a 250 ml conical flask kept in water bath for alkali treatment. 75 ml of 17.5% caustic soda was measured using a measuring cylinder at 20°C. 15 ml of 17.5% NaOH was added and fibres were macerated gently with a flattened glass rod for 1 minute. 10 ml more NaOH was added and the solution was mixed for 45 seconds. 10 ml NaOH was again added and mixed for 15 seconds to make lump free slurry.
a. Count the number of dead brine shrimp instead of living brine shrimp b. Add more brine shrimp to the water with the highest salt concentration c. Repeat the experiment several times, and calculate the average number of brine shrimp d. Reduce the length of the experiment from 24 hours to 8 hours. 23. Where is the information stored in DNA?
After deciding on what algae concentration we wanted to hold constant in our jars, we calculated the volume of seawater and algae solution required to achieve the desired algae concentration of 50,000 cells/mL and a total volume of 50 mL (C1V1=C2V2). We measured out the required volume of seawater with a graduated cylinder, pouring the contents into each jar. Because our calculated seawater volume was not a whole number, we utilized a micropipette to transfer the remaining amount of seawater. We then proceeded to collect algae from the large flask after carefully swirling around the algae to evenly distribute it within the water (Flipped Lab Videos 2016a). Using the micropipette, we transferred the appropriate volume of algae to the jars.
100ul of Folin reagent was added to the wells and incubated for another 20 minutes. The absorbance was measured at 630-700nm. A standard curve of absorbance vs. protein concentration was plotted and the protein concentration in the diluted sample and the total percentage of activity were
As it was done in the Experiment A, 20 drops of 0.2 M acetic acid and 10 drops of 2% starch solution was mixed well with the juice solution. Before adding the iodine solution, the initial reading of the burette was taken. Then, the titration was started using the iodine solution into the burette with continuous swirling of the flask slowly and carefully. Once the color change started to appear, titration was stopped and final burette reading was recorded. Finally, the amount of vitamin C in the mandarin orange was calculated by using the standardization factor and used iodine solution.
If the concentration of glucose in the blood is low / below the normal range, it leads to the secretion of glycogen from the alpha cells. Glycogen will change the energy stores such as glycogen in the liver to the glucose (stimulates the breakdown of glycogen) by increasing the level of sugar in the blood(ibid).
22.214.171.124.2 The performed toxicity test principle: The preformed test in this experiment is a modified method of the limit test OECD TG 203, and is more adapted for marbled crayfishes. The principle of the test is to show that the LC50 is greater than 100 mg /l. In this test, the marbled crayfishes were exposed to the different test substances dissolved in water and observed for 120 hours. Ever 24 h the mortality, the growth, color and the movement of the crayfishes were recorded. When 1 crayfishes of the test group dies, the test should be terminated and a complete OECD TG 203 should be performed.
The oxidation of these molecules is primarily used to transform the energy contained in these molecules into ATP. ATP os a large source of energy for muscle contractions and can therefore be referred to as "energy currency" of the cells. The fuel molecules is first converted into acetyl-CoA and then can be inserted in the Krebs Cycle. Looking at the path of a nutrient, such as glucose, the oxidation of the molecule takes place in the glycolysis. The product of the glycolysis is pyruvate.
It was equilibrated with tissue for 10 minutes by flushing into the organ bath. After that, the steps above were repeated to test tissue response using 5ml of 1 x 10-5M and 1 x 10-4M of mepyramine. The experiment was repeated by replacing mepyramine with SIPBSDrug A as the antagonist. Lastly, concentration-response curve with Hill-Langmuir equation and Schild Plot were plotted using Bio-Graph. KB and pA2 values for mepyramine and SIPBSDrug A were calculated based on Schild plots and Gaddum
The preparation of the dilutions is a multi-step process in which the dilution is appropriate for a 250 ml sample of algae water. Chlor Brite: Dissolve 8 grams of the powder into 4 liters of tap water Remove 1 ml of this solution Add this to 1 liter of water Remove 1 ml of this solution to add to samples in the D group Power Powder Plus