Dna Fingerprinting Lab Report

DNA Fingerprinting Using Agarose Gel

S. Aaron Sowards

Bio 122 Lab 04

Brianna Adanitsch

Jakob Lester

Minhenga Ngijoi

2/21/18

Dr. Chad R. Sethman

Abstract

DNA fingerprinting is the process of analyzing an individual’s DNA base-pair patterns. The DNA fingerprinting lab involved identifying the suspect using Agarose Gel and Polymerase Chain Reaction. It was found that suspect two s DNA matched the crime scene DNA. This is known because suspect twos DNA traveled the same distance as the crime scene DNA.

DNA Fingerprinting Using Agarose Gel

Introduction

In 1984 Dr. Alex Jeffreys came up with deoxyribonucleic acid (DNA) fingerprinting, which is also known as DNA profiling or DNA typing. DNA fingerprinting is the analyzing

The agarose gel was placed into the electrophoresis chamber and covered with Electrophoresis buffer. The ruler was inserted with a pipet into cell one of the gel. The DNA from the crime scene that was cut with enzyme one and two was then inserted into wells two and three. Suspect one and two’s DNA where cut with enzyme one and two. Suspect one’s DNA was inserted into wells four and five; suspect two’s DNA was inserted into wells six and seven. The apparatus was then closed and turned on to run at 100 volts. Electrophoresis ran for 30 minutes; separating the DNA according to size in the gel. Then the tray with the gel was removed and a stain sheet was placed on the gel for 15 minutes. After 15 minutes that gel was rinsed with water and then set in water. Later, 20 to 30 minutes, the gel was view over a light box to view how far the bands traveled. The distance the bands traveled was measured in

This was achieved by identifying the suspect. According to the gel, suspect number two’s DNA was found at the crime scene. This is known because the crime scene DNA and suspect two’s DNA both traveled the same distanced. And said before DNA is very polymorphic; no two pieces of DNA will be the same when cut by the same enzyme (Edvotek, 2014). This is also shown in Hyun-Jung Choi’s lap report. In the lab report three students are tested along with one suspect. Student number two’s DNA matched the suspects DNA. The student’s DNA’s are cut with five different enzymes as well as the suspects DNA. Student two’s DNA matched exactly with the suspects DNA; the other two student’s DNA did not resemble the suspects DNA at all. (Choi, et al, 2008) DNA fingerprinting is used a lot in determining who committed a crime. Although, in our lab report, suspect ones DNA matched the crime scene when cut with enzyme one, this can be explained by how closely related the two suspects are. Therefore two enzymes were used to cut the DNA; the suspect has to match both. Moreover, the limitation to DNA fingerprinting is, if a person were to have an identical twin. This is because identical twins have the same DNA because they come from the same egg. If a suspect’s DNA matched that of the one being tested, and they had an identical twin, a farther investigation would need to be done. DNA