Polymerase chain reaction Essays

  • Polymerase Chain Reaction

    771 Words  | 4 Pages

    Polymerase Chain Reaction (PCR) Polymerase chain reaction (PCR) is a laboratory technique used to make various duplicates of a portion of DNA. PCR is very exact and can be utilized to intensify, or duplicate, a particular DNA target from a blend of DNA molecules. It empowers scientists to create a huge number of duplicates of a particular DNA arrangement in around two hours. This robotized procedure sidesteps the need to utilize microscopic organisms for intensifying DNA. First Stage: The reactants

  • Polymerase Chain Reaction Report

    1219 Words  | 5 Pages

    1.Polymerase chain reaction (PCR): Polymerase chain reaction is a method of DNA or RNA amplification . The PCR method allows millions of copies to be created from a very small DNA section. The PCR methodology was developed in 1983 by Kary Mull , who in 1993 received a Nobel Prize for Chemistry with Michael Smith PRINCIPLE & PROCEDURES: 1.DNA denaturation. Once the DNA has been isolated and purified from the cell, a PCR assay can begin. Uncleaned DNA can also be used for PCR, but it is ineffective

  • Polymerase Chain Reaction (PCR)

    1158 Words  | 5 Pages

    Polymerase Chain reaction (PCR) Principle: PCR is a process which involves taking a DNA template and amplifying distinct regions of it in vitro. To conduct PCR you need the DNA sample, DNA primers( two because one is forward and one is a reverse primer), Deoxynucleoside triphosphate bases(dNTP), DNA (Taq) polymerase, a buffer and some cations (mg2+). The reaction is carried out in a thermal cycler which fluctuates the temperature to allow progression of the amplification. Procedure: Initially the

  • Polymerase Chain Reaction Process

    906 Words  | 4 Pages

    The polymerase chain reaction or known as (PCR) is a scientific technique used in molecular biology to amplify a specific DNA sequence.It can be used very quickly and efficiently to produce millions or billions of copies of single DNA sequence. Polymerase chain reaction or PCR uses repeated cycles of heating and cooling to make the copies of specific DNA. High temperature is necessary to break weak hydrogen bond that binds the two stands of DNA together and as a result multiple copies of a

  • Polymerase Chain Reaction Essay

    1132 Words  | 5 Pages

    Polymerase Chain Reaction (PCR) is a laboratory technique used in molecular biology to generate a small section of DNA or genes, PCR uses primers to amplify specific genomic DNA sequences with the help of special enzymes, PCR process uses short sequences of DNA and primers and selects specific chromosomes of DNA for replication. (McPherson and Møller, 2009) In addition, there are three main steps involve PCR process, first step of PCR is denaturing when DNA is heated to 90-95 degrees Celsius and

  • 2.7 Polymerase Chain Reaction (PCR)

    716 Words  | 3 Pages

    2.7 Polymerase Chain Reaction (PCR) In 1971, Dr. Har Gobind Khorana’s group described the idea to amplify a DNA (Templeton, 1992). Later on in 1985, Kary B. Mullis invented the Polymerase Chain Reaction (PCR) which is used today in different fields, such as scientific research, clinical diagnostics and criminal investigations. PCR is a molecular biology tool that is used to amplify a fragment of DNA to generate thousands to millions of copies. This technique is based on an enzymatic reaction which

  • Pcr Analysis Of Minced Meat Sample

    4017 Words  | 17 Pages

    Analysis of minced meat samples using Polymerase Chain Reaction (PCR) and Gel Electrophoresis to investigate adulteration in the product Food Traceability and Genomics John Barry   Title: Analysis of minced meat samples using Polymerase Chain Reaction (PCR) and Gel Electrophoresis to investigate adulteration in the product Name: John Barry Date: 7/10/14 Aim: The aim of the experiment was to examine minced meat samples for adulteration by amplifying extracted DNA using a PCR method. Gel

  • Myrtle Rust Fungus

    670 Words  | 3 Pages

    The key enzyme in the process is DNA polymerase that forms the PCR product by linking individual nucleotide bases (adenine, guanine, cytosine and thymine). Short DNA fragments with sequence complimentary to target DNA are called primers; these specify the DNA sequence that has to be amplified. The PCR reaction mixture is made in a 96 well plate or test tube and placed in a thermal cycler. The thermal cycler is a machine used

  • Pilcher's Trial: Guilty Or Inhumane?

    1580 Words  | 7 Pages

    Background On April 9th, 1974, a young woman at the age of 17 was found in a farmhouse in Blakesburg, Iowa. Her name was Mary Jayne Jones, and she had been sexually assaulted and shot in both her heart and head at close range with a high-powered rifle. Miss Jones was originally from North Carolina, but had moved to Iowa to assist her expectant sister, Mrs. Pat (Jacque) Williams, but decided to stay. At the time, she was working at Henry’s Drive-in restaurant in Ottumwa, Iowa. Mary was living with

  • Dna Fingerprint Case Study

    350 Words  | 2 Pages

    Describe how a technician would collect a fingerprint from a weapon that could possibly have touch DNA on it as well as fingerprints. How would you collect the possible DNA? Which would you collect first? As we go about our day we inadvertently leave behind our unique friction ridge impressions in items we come in contact with. Within those impressions, sebaceous secretions, eccrine sweat and apocrine sweat reside on our pores containing our individualized DNA. Therefore, small traces of DNA in

  • Roche 454 Unit 3 Sequencing

    944 Words  | 4 Pages

    fluorescence was detected and the extra tail with fluorescent probe is cleaved out. After multiple cycles of ligation, detection and tail cleavages, the extended chain reached the end of the template. Then the whole extension chain is removed and a new starting primer switching down 1 nucleotide position binds onto the template for another cycle of reaction. Totally, five round of primer binding cycles are performed to complete the sequencing of each fragment. 3. Pitfalls and limitations of NGS Errors could

  • Dna Fingerprinting Lab Report

    1726 Words  | 7 Pages

    Lester Minhenga Ngijoi 2/21/18 Dr. Chad R. Sethman Abstract DNA fingerprinting is the process of analyzing an individual’s DNA base-pair patterns. The DNA fingerprinting lab involved identifying the suspect using Agarose Gel and Polymerase Chain Reaction. It was found that suspect two s DNA matched the crime scene DNA. This is known because suspect twos DNA traveled the same distance as the crime scene DNA. DNA Fingerprinting Using Agarose Gel Introduction In 1984 Dr. Alex Jeffreys

  • Unit 3 Lab 3 Dna Extraction And Visualization

    1241 Words  | 5 Pages

    Lab 3 – DNA extraction and visualization Journal -Madhu Thalari. 1.Describe the laboratory exercise as you interpreted it.? Ans: This lab has given me methods to extrct DNA from both plant cells and animal cells. The main steps that are followed in both methods made me understand

  • Acheta Lab Report

    876 Words  | 4 Pages

    Experiment and Results Sample 100 ng/µl DNA was extracted from the cricket Acheta domesticus using the phenol-chloroform methods described in Davies et al., 2012 [15], dissolved in Tris-HCl-EDTA (TE) buffer and kept frozen at -20˚C. In initial tests, portions of the extracted DNA were suspended at the same DNA concentration as the control sample in solutions of magnesium chloride, magnesium sulfate, ammonium sulfate, lithium chloride, and nickel chloride. Each salt was mixed in three different concentrations

  • DNA In Forensic Science

    1116 Words  | 5 Pages

    DNA in Forensic Science DNA is the carrier of genetic information in humans and other living organisms. It has become a very useful tool in forensic science since it was discovered. In forensic science, DNA testing is used to compare the genetic structure of two individuals to establish whether there is a genetic relationship between them. One example of the use of DNA in forensic science that is important in biology today is comparing a suspect’s DNA profile to DNA that was discovered at a crime

  • Gel Electrophoresis Lab

    502 Words  | 3 Pages

    Agarose gel electrophoresis is an easy and common technique of separating and analyzing DNA. The main objective of this lab is to find the sire of the offspring using gel electrophoresis. Gel electrophoresis is used in laboratories to isolate charged molecules like DNA, RNA, and particular proteins according to their specific size. The charged molecules travel through the gel when an electric current is spread across it. The electric current is applied across the gel so that the ends of the gel have

  • Gel Electrophoresis Lab Report

    816 Words  | 4 Pages

    Abstract: Gel electrophoresis is a method used to separate DNA fragments according to size. During our study we pondered on one particular question; whose blood was left at the crime scene in the AP biology classroom? Before carrying out our experiment we learned about the process of gel electrophoresis and the use restriction enzymes. After analyzing our results, we decided to reject our hypothesis because our experiment showed strong evidence against what we originally hypothesized. Introduction:

  • Ligation Lab Report

    2038 Words  | 9 Pages

    Ligation The objective of this experiment was to ligate EGFP DNA inserts into pET41a(+) plasmids. A total of five ligations were performed, two actual ligations and three control ligations. The following reagents were utilized: Nco I/Not I cut pET-41a(+) DNA 50 ng/μL, EGFP cDNA insert 7 ng/μL, uncut pET-41a(+) DNA/EGFP recombinant plasmid DNA 25 ng/μL, ligase buffer 10X, and ligase. To prepare ligation #1, a 1:1 molar ratio of pET41/EGFP, 2 μL digested pET41a(+) DNA, 1 μL EGFP cDNA insert, 14 μL

  • Trypsin Digestion Report

    1565 Words  | 7 Pages

    bicarbonate at room temperature for 30 minutes reduced the protein disulphide bonds. Proteins were then digested with 4µg of sequencing grade-modified trypsin (Sigma) in 50 mM ammonium bicarbonate by incubating overnight at 37ºC. The trypsin digestion reaction was stopped by 1µl of 100% formic acid. The digested peptide solutions were centrifuged at 14000

  • Aliivibria Lab Report

    251 Words  | 2 Pages

    light production[1].Aliivibrio fischeri also forms a symbiotic relationship with animal hosts[3]. Aliivibrio fischeri utilizes the nutrients provided by its host to emit light that is later used by the host for various purposes[3]. The light emitting reaction of Aliivibrio fischeri is catalyzed by