The first well was pre-loaded with DNA ladder and labeled as 1 microliter kb DNA ladder. Next one microliter of DNA was mixed with one microliter of loading dye using a pipettor and loaded into the well. The same mixing process was completed for the PCR product, using one microliter of PRC product and one microliter of loading dye. For the purposes of this experiment, the DNA product was loaded into well six and the PCR product was loaded into well seven. Initially DNA was loaded into well five, however gel was pierced so samples were moved one well to the right.
DNA Restriction Mapping Danielle Niemeier email@example.com BCH 467 Analytical Biochemistry Lab Section: 16371 Abstract The purpose of this experiment was to determine whether the vector PRSETB or pQE30 is present and to create a restriction map for the unknown plasmid A. The plasmid A was digested with enzymes BAMH1, PstI, and ScaI and then the resulting fragments were run through an agarose gel via electrophoresis. From the gel electrophoresis and deriving an equation by plotting the log of the size of the DNA size markers and distance migrated, a restriction map was constructed. The restriction map showed that the plasmid has only one ScaI site, which supports that vector PRSETB, is present in the plasmid. From the gel electrophoresis, it
There are many similarities between TIMEX3 and TIMEX2 and it is possible to convert from TIMEX3 TO TIMEX2 tags, even if some attributes are not supported. Similar to the transformation from TIMEX2 to TIMEX3 described by , though the other way around. This conversion method helps the temporal tagger to use TIMEX3 annotated corpora for evaluation. HeidelTime’s Architecture The most important feature HeidelTime architecture is the strict separation between the algorithmic part, i.e., the source code, and the resources for patterns, rules, and normalization information. HeidelTime resources are organized in modular manner.
coli bacteria new traits. The pGLO plasmid that is being transformed into these cells contains genes that can give colonies of bacteria the ability of antibiotic resistance and a green fluorescent glow. Four different models were prepared and plated on multiple agar plate. After the bacteria was grown for three days in an incubator at 37°C; observations were made and recorded (Table 1). All of the plates were looked at for the amount of colonies grown, if growth was present, and if the colonies gained the ability to glow green.
The purpose of this experiment is to create a complete genomic library of Aliivibrio fisheri through the use of the lux operon. The examination of the lux operon gene occurs through the extraction of the DNA of Aliivibrio fischeri and digest a large piece of DNA to smaller random pieces. The fragment of DNA will later be ligated together in plasmid. Plasmid acts as vectors to transport DNA from one organism to another. The DNA will then run through a UV-visible spectrophotometer to test the absorbance of the extracted DNA.
The key enzyme in the process is DNA polymerase that forms the PCR product by linking individual nucleotide bases (adenine, guanine, cytosine and thymine). Short DNA fragments with sequence complimentary to target DNA are called primers; these specify the DNA sequence that has to be amplified. The PCR reaction mixture is made in a 96 well plate or test tube and placed in a thermal cycler. The thermal cycler is a machine used to amplify DNA by altering the temperature in repeated cycles using three precise pre programmed steps: Denaturation – By increasing the temperature in the thermal cycler the DNA is denatured creating separate single stranded DNA
Next step is translation, in which the RNA becomes a protein, which then can act as structural units or enzymes. 2. How does DNA replicate itself? In order to replicate itself, DNA undergoes DNA replication, a process in which the DNA unwinds and splits in two. From that point on, new nucleotides are added to each of the original strands (A to T, C to G) until the result is two identical sequence copies of DNA.
The organism that was successfully cultured from the first lab was use to streak into other plates. Samples from the previously cultured MAC and blood agar were streaked on two different sides of an EMB, SS, and MSA plate. Samples of bacteria were also used to inoculate Citrate and TSI media. The inoculation of TSI and Citrate media were as follows: The materials were gathered, which include the previously cultured media, an inoculation needle and the Citrate and TSI medium. This procedure occurred in the presence a Bunsen burner.
We then flipped the dish and sectioned it off into 4 sections, which then were marked with the specific genotype that would be inoculated into that section. The initials of the group were also put on the dish. Then we used an inoculating loop to cut out sections of the fungus. The inoculating loop was sterilized with a flame and let cool down before touching the fungus. After cutting a block of the fungus, we placed it on the petri dished in the section that was appropriately marked for that specific strain.
Remove excess water from the surface of the gram stained glass slide and observed under 1000X oil immersion microscope. Gram-staining have performed for Staphylococcus aureus (control); Enterococcus faecalis (control); Nostril microflora in NA, MSA, and PYCa. Gram-staining provides results of the bacteria morphology, type of gram-stain. Catalase test was also done prior to gram-staining. MicrobactTM Biochemical Identification Kit was used for identifying gram-negative aerobic and facultatively anaerobic bacteria.