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Essential Oil Research Paper

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CHAPTER ONE INTRODUCTION Introduction Essential oil still holds its popularity over the centuries as it was utilized in many fields. For example in odorants industry (perfumes, fresheners, detergents and paints), pharmaceutical field (anti depressants, anti anxiety and anti septic) and in food industry (food flavour, addictive and preservatives). The uses of essential oil are known for its variety in chemical composition that makes up diversity in its properties. From the researches, essential oil contains many different components such as hydrocarbons, vitamins, alkaloids, steroids, flavanoids and vitamins. These compounds may bring benefits to us but also can cause harms. This is why the study regarding the essential oil is important …show more content…

The fresh plant materials of flowers will be collected locally during these 2 semesters of study. Gas Chromatography-Flame Ion Detector will be use to analyze the chemical components present in the essential oil. Bioassays that will be conducted to the essential oils are toxicity test and antioxidant …show more content…

were collected from CTF 1, Faculty of Resource Science and Technology, and Tasik Ensurai Universiti Malaysia Sarawak (UNIMAS) respectively while Etlingera elatior and Rosa were purchased from nearby market and flower shop. Extraction of essential oil The essential oil was extracted from fresh flower part (200 g) by hydrodistillation for 6 hours on a Clevenger-type apparatus. The extraction of essential oil was done in tripilicate. GC/FID analysis The analysis was carried out by splitless injection of 0.1 µL of isolated volatile oil into a PerkinElmer Clarus 680 , gas chromatograph equipped with a flame ionization detector (FID) and a CP Sil 8CB fused silica capillary column (30 m, 0.32 mm i.d., 0.25 µm film thickness). Nitrogen was used as the carrier gas at a flow rate of 1 mL/min. Oven temperature programme: injector temperature 280℃, oven temperature 280℃ at 30℃ /min, detector temperature 280℃. Relative percentages of individual components of the oil were obtained from the GC-FID peak area-percent report. Biological activity test Brine shrimp toxicity

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