2. Materials and methods 2.1. Materials Analytical grade Fe(NO3)3.9H2O, Na2SO4, NaHCO3, and H2SO4 were obtained from Merck. Reagents such as pluronic acid P123, hydrochloric acid and tetraethylorthosilicate (TEOS) were collected from the Sigma Aldrich and Rhodamine B was purchased from Loba Chemie chemical company. These reagents and dyes were directly used without further purification. Required working volume of RhB dye was made by diluting the prepared 1g/l stock solution. Graphite plates purchased from Anabond Sainergy Limited, Chennai, were used as anode as well as cathode in all electrolytic experiments. 2.2. Synthesis of Fe-SBA-15 The heterogeneous catalyst Fe-SBA-15 was synthesized by wetness impregnation method. The detailed procedure adopted for the synthesis of Fe-SBA-15 and characterization results were previously reported by Sanjini and Velmathi (2014). Ferric nitrate nonahydrate and TEOS were used as iron and silica sources, respectively. The basic mesoporous SBA-15 catalyst support was prepared according to the method previously described by Zhao et al., (1998). The first step was to disperse 4 g of block P123 (EO20PO70EO20) in 40 ml of distilled water and 120ml of 2 M aqueous HCl was added with stirring at 350 C for about 3 h. To this solution, 4 g of TEOS was added and continuously stirred at 400 C for 24h. Then the resulting gel was …show more content…
The procedure adopted to find the leached concentration of ferrous ions and ferric ions in the electrolyzed RhB solution was already reported by Nidheesh et al. (2014). Reusability of catalyst is one of the major advantages of heterogeneous catalyst (Panda et al., 2011; Zhang et al., 2015). After each experiment, the used catalyst was filtered off and dried at 1050 C for 24 h. This recycled catalyst was utilized for further studies and experiments were conducted at conditions similar to that carried out for the raw
While the solution dissolved, 50 mL of distilled water was added to a 150 mL beaker and heated on the hot plate. When the solution started to boil 2.65 grams of Na2SiO3*5H2O was added to the beaker with a stir bar and heated to a gentle boil. When both solutions began to boil, the sodium silicate solution was slowly added to the sodium aluminate. The solution was kept at 900C for 60 minutes and stirred with stir bar. After 60 minutes, the zeolite solution was cooled for 5 minutes and for the magnetized zeolite , 0.78 grams of FeCl3 and 0.39 grams of FeSO4*7H2O was added to the flask and stirred until the iron parts dissolved.
3.1 Project Methodology In order to make sure that this project is on track, proper planning and scheduling is crucial. It is necessary to identify the significant key points which are photocatalytic activity of SWCNTs, properties of carbon nanotubes and titanium oxide, available testing procedures using scanning electron microscopy (SEM) and other spectroscopic methods. In this section, a brief detail provided regarding the methods used, sol-gel, hydrothermal and electrospinning. Also, the available tests that will be used are explained.
That mixture was then filtered through a coffee filter. Nine test tubes were prepared in order to perform this dye coupled reaction. One contained 5.0ml of the potato and pH buffer mixture, 2.0 ml of hydrogen peroxide, and 1.0 of guaiacol to serve as a blank for the spectrophotometer. Four test tubes were filled with 2.0 ml of hydrogen peroxide and 1.0 ml of guaiacol, used for measurement by the spectrophotometer, each. The last four were filled with 4.0 ml of the potato and pH buffer mixture and 1.0 ml of peroxidase.
The anion tests followed the cation tests. To test for the presence of the chloride (Cl-) anion, a small scoop of the unknown compound was mixed with 1 mL of water in a test tube to create a solution. Then, 1 mL of 6 M nitric acid (HNO3) and 1 mL of silver nitrate (Ag(NO3)2) solution were added to the test tube to see if a white precipitate formed. To test for the presence of the sulfate (SO42-) anion, a small scoop of the unknown compound was mixed with 1 mL of water in a test tube to create a solution. Then, 1 mL of 6 M hydrochloric acid (HCl) and 1 mL of barium chloride (BaCl2) solution were added to the test tube to see if a white precipitate formed.
The hypothesis that was provided to this question was If Phenol Red is add with the other chemicals then a color change will occur. Methods: To begin the lab 40 mL of Phenol red were obtained in a beaker, and 40 mL of water were obtained in a separate beaker. Next one plastic baggie was
The setup for the cation exchange chromatography is shown in Figure 3. This was done by plugging the bottom of a burette with a small amount of glass wool. The wool was lightly packed using a thermometer. Approximately 5 mL of Dowex 50 cation exchange resin was obtained in a small beaker, and the resin was mixed with 5 mL of pH 3 citrate buffer. This mixture was poured into the burette with the stopcock closed.
There were solid pieces produced that were a dark reddish color. This change in color shows that there must have
Pure ASA crystals are isolated from the solution with a Hirsch Funnel that was used with a filter. The melting point of the pure ASA crystals were calculated in order to calculate of absorbance. Iron (III) salicylate dianion must contain the acidified solution Fe3+ in order to measure the absorbance values. The level of the impurity can
Abstract: The purpose of this experiment was to identify given Unknown White Compound by conducting various test and learning how to use lab techniques. Tests that are used during this experiment were a flame test, ion test, pH test, and conductivity test. The results drawn from these tests confirmed the identity of the Unknown White Compound to be sodium acetate (NaC2H3O2) because there were no presence of ions and sodium has a strong persistent orange color. The compound then will be synthesized with the compounds Na2CO3 and HC2H3O2 to find percent yield.
The reagents used were Diphenylamine reagent which contains concentrated H2SO4. The standard solution used for this test is the deoxyribose standard solution. In the sample, only a faint blue solution appeared, which indicates a small presence of deoxyribose. In test for Phosphate, the standard solution was the Phosphate solution and the reagents used were concentrated H2SO4, concentrated HNO3, 2.5% ammonium molybdate solution.
Then multiples of the number of moles of Fe+2 were chosen for the moles of phen in each solution. Since the solutions were made in a 50 mL volumetric flask we then used M = mol/volume again to figure out the molarity of each reactant in the solution. Finally, the solutions were also diluted again in a 1:10 using the formula M1V1 = M2V2, where the V2 was 10 mL so the absorbance values would be acceptable. This made sense as the solutions were an initially a deep red color and would thus need greater dilution to reach the appropriate absorbance values.
With the mechanism understood, the experimentally was able to be performed by mixing about 5.55 grams of NaBr with 5mL of water and 5mL of 1-butanol and mixed thoroughly. Then the sulfuric acid was mixed slowly into the solution with the flask in an ice bath since the acid-base reaction is exothermic, which generates heat. With the reaction set, the solution was then prepared for
This reflects the variability in toxicity of naturally occurring LPS (rapidmicrobiology n.d.). The chromogenic method uses a synthetic substrate that brings about a colour change when it is cleaved by endotoxin-activated protease. The turbidimetric method on the other hand, relies on the a coagulin gel cot forming which will alter the turbidity of the sample. Both the turbidimetric and the chromogenic methods can be used as quantitative kinetic methods simply by plotting standard curves of time vs endotoxin concentration. Spectrophotometric instruments can be used to detect changes in colour and turbidity at much lower concentration than that need to form a visible gel-clot.
The dried roots of Inula racemosa were pulverized and sieved with 100 ~ 200 mesh. The herb powder was placed into a glass bottle. Ultrasound-assisted extraction was carried out in an ultrasonic cleaner RK102H (Bandelin sonorex, Germany). The powder of Inula racemosa was extracted three times under the following conditions: the ratio of material to solvent was 10:1, undergoing ultrasonic treatment 30 minutes at 25 °C, 100 kHz /450 W.31 Before large extraction, a small-scale extraction experiments were carried out: 95% ethanol and ethyl acetate as the extractive solutions was investigated, respectively.
Paragraph 1 The research paper talks about how the temperature of formation and crystallinity of iron phosphate, FePO4, is critical in determining its electrochemical behaviour. FePO4 is known to crystalline in several different structures. At 600 degrees, FePO4 irreversibly changes into an electrochemically inactive quartz-like structure, which shows that the olivine form is metastable. FePO4 at a high temperature is limited to measurements of call parameters. In the case of α-phase FePO4, cell parameters tend to increase exponentially as temperature increase.