Tlc Lab

Rinse the Erlenmeyer flask with about 10 ml of solvent and pour the solvent through the funnel, too. Remove the funnel, add two or three boiling chips and reattach the thermometer and adapter to the still pot. • Discard the magnesium sulfate remaining in the Erlenmeyer flask by dissolving it in tap water and pouring the solution down the drain. • Before beginning the distillation, weigh a clean, dry 1 narrow mouth screw cap bottle on a balance. Remove the cap of the bottle, and insert the clean, dry plastic long-stem funnel in the neck of the bottle.

the settle process is all about adding a polymer to join with small solid particles then it settle down and in the top almost pure water is existed. The pure water is dosed into the line with out powder to avoid the deposition in the line. There is an extraction pump used to take the settled powder for recycling by adding water or to the sludge

Step 2: Mix both test tubes , shake gently and time the reaction. Step 3: The same step as procedure 1, and step 3 which is to record the observed color step 4: use the palette/color chart to help you identify the observations you make. Safety precautions: Pull your hair back Safety eye goggles Closed toe

The samples were filtered by vacuum over pre-weighed and wetted glass fiber filters (24 mm GF/C; Whatman; Kent, UK). The samples on the filters were rinsed with 5 ml stop solution to remove non-specific, cell surface binding of 3H-FLC. The filters with fungal balls were either allowed to dry for 24-48 hr or were baked in a drying oven for 15 minutes at 95° C. Each dried filter containing fungal balls was then re-weighed to obtain the dry mass of each fungal sample. The filters were finally transferred to 5 mL scintillation vials containing 3 ml of scintillation cocktail (Ecoscint XR, National Diagnostics, Atlanta GA).

Washability of the gel was determined by the following method. A small quantity of gel was applied on the skin. After washing with water it is checked for whether the gel was completely washed out or not. Spreadability was determined by using modified wooden block and glass slide apparatus. A measured amount of gel (0.5 g) was placed on fixed glass slide with a circle of 1cm diameter; the movable pan with a glass slide attached to it and was placed over the fixed glass slide, such that the gel was sandwiched between the two glass slides for 5min.

Prepare the standard solution of FeSCN2+ pipetting 18 mL of 0.200 M Fe(NO3)3 in a 20 * 150 mm test tube labeled five. Pipet 2 mL 0.0020 M KSCN into the test tube and stir. 6. Prepare a blank. Fill the plastic cuvette ¾ full with distilled H2O. Properly clean the cuvette and handle only by the top ribbed edge while wearing gloves.

The developing solution was poured into a tank and was tightly covered with a glass lid, and the tank was allowed to be saturated to ensure that the solution was equilibrated in the gas phase. Silica plate for TLC analysis: A horizontal line was drawn with a pencil on the plate and it was about 1 cm above the bottom of the plate. The horizontal line was drawn faintly so as to avoid damaging the silica gel on the plate. On the horizontal line, two marks were made and one was named A and the other B. These marks were made towards the centre of the plate at a distance apart because when spots are made at the edge of a plate, the result would be an improper travel of the samples as the solvent advances on the plate.

With your partner, decide what four cultures you would like to study (i.e., cell phone, table top, door handle, etc) 5. In your notebooks document what quarter of the petri dish contains which culture: Example: A – door handle 6. Procedures for taking a culture: Take one cotton tipped applicator and dip into the sterile water.

With a fresh pipette we removed 1ml from tube 4 and added it to tube 5 and gently we shake the tube and another fresh pipette we removed 1mlfrom tube 5 and discarded this fluid. Tube number 1to 5 had the same amount of fluid in them but the concentration of protein was becoming weaker as we progressed to tube number 5 and this is what we used to develop our standard curve. In the tube that we labelled “U” we added 1ml of unknown protein and into the tube labelled “B” we added 1ml of water. At this point we had six tube with water and protein solutions, the “U” with unknown protein and water but tube 1 to 5 with a standard protein solution and water.

We have two other tasks to accomplish in the lab we must determine the denisty of the vinegar sol and the molarity of the acetic acid in a vinegar The mass percent can be our data. To accomplish our tasks, we will need to make very accurate volume and mass measurement .Burette and pipettes are useful in accurately measurement PROCEDURE: Prepration of Burette 1 .check the burette by rinsing down the sides with distilled water bottle to check if water sheets down inside of the burette If water droplets are observed, the burette should be washed before use. Be careful not to scratch the inner surface if you find it necessary to use a burette brush to clean it Rinse the burette well with tap water including the stop cock and washers.

When no more solids were dissolving, the mixture filtered by gravity using a pre-weighed filter

After the cover slip was in placed we then got microscopes and set the stages and lighting to view the specimen. The spores were to be observed at a 4x magnification or 10X to see the color. We were to observe and record 30 asci and enter on computer spreadsheet, but asci with 8 spores of identical color were to

Next one microliter of DNA was mixed with one microliter of loading dye using a pipettor and loaded into the well. The same mixing process was completed for the PCR product, using one microliter of PRC product and one microliter of loading dye. For the purposes of this experiment, the DNA product was loaded into well six and the PCR product was loaded into well seven. Initially DNA was loaded into well five, however gel was pierced so samples were moved one well to the right. The gel was run at 100 V for one hour.

Introduction The purpose of this week’s lab was to enhance our understanding of the Grignard reagents that were examined in lecture. In this lab, a Grignard reagent will be prepared through the reaction of magnesium turnings and bromobenzene. Instead of isolating the product it will then be combined with benzophenone, which will give the final product of triphenylmethanol. Procedure

The objective of this experiment was to create synthesize methyl eugenol from eugenol, dimethyl carbonate, and tetrabutylammonium bromide. To start off the experiment, a heating under reflux apparatus was used and the parts included: a water jacketed condenser, ring stand, tubes, flowing water, 25-mL round bottom flask, heating block, and a hot plate. There were two parts to the water condenser, entry and exit ways for water. The bottom opening was connected to the sink through one tube and the top opening was connected with a loose end, which was needed to get rid of the flowing water. To create the solution needed to synthesize methyl eugenol, approximately 0.200 g of eugenol (note: the measured g was converted to mg for later calculations) was measured, alongside approximately 1.2 g of TBAB and was added to the 25-mL round bottom flask.