The fastest pH was 6 (total:34.5), and it seems that there wasn’t a large change which resulted in a stable structure. The temperature in our experiment was not very high which didn’t result in denaturation of peroxidase. The temperature seemed to be a constant that didn’t affect the experiment. If the temperature was higher in pH 3 and low in pH 10, then it would cause pH 3 to denature even more which would make the pH 3 total about 4.0. Substrate concentration basically means the amount used for the substrate.
Sodium Bicarbonate mixed with Hydrochloric acid. The chemical reaction observed showed that there was fizzing and bubbling, this is evidence that a new gas was being produced. This new gas, CO2 was generated from the reaction. After the fizzing stopped a liquid was leftover leading me to conclude the liquid leftover leading me to conclude the liquid leftover was the NaCl and H2O 4. You found a sample of a solution that has a faint odor resembling vinegar (an acid).
On the other hand, 132 seconds were needed for the solution with the crushed tablet to stop reacting, which is the one with the reactant having a larger surface area. In a reaction, the absence of fizzing indicates that the chemical process has ended. Average rate of reaction (Whole Tablet) = 100 / 160 = 0,625 Average rate of reaction (Crushed Tablet) = 100 / 132 = 0,757 0,757 > 0,625 All these information signifies that the powdered vitamin C increased the reaction rate by causing the reaction to take place in a decreased amount of time. From the graph it is also clear that the rate of reaction took place with a fairly higher speed when the powdered vitamin C was used. Based on the previous results, the hypothesis was confirmed.
According to Clare (2002), pH between values of 6.5 and 9.5 is acceptable by daphnias, with the optimum being somewhere around 7.2 to 8.5. When ethanol was tested on the daphnia, observations show slow movements and decreased heart rate by the daphnia. Explanation for this is because alcohol is known as a depressant for the nervous system by acting as a non-selective neurodepressant which also affects humans heart rate in the same way (Handy, 2009). When daphnia was placed in salt solution, it showed fast, increased movements and increased heart rate until gradually stopped completely after a while of exposure to salt solution. This is because salt solution is a hypertonic solution which creates a gradient of solute concentration when daphnia is immersed in the solution and draws water molecules out of the cells of the organism resulting in shriveling of the cells of the daphnia, also called crenation, which finally leads to the death of the daphnia (Carter-Edwards et al,
CHROMATOGRAPHIC METHODS: After successful extraction of phospholipids from their source analysis can be performed for the detection of specific phospholipids. This section will discuss chromatographic methods used for the analysis of phospholipids. All systems of chromatography consist of a stationary and mobile phase. A monster placed on a stationary phase, i.e., a solid or a liquid, and the mobile phase, a gas or a liquid, is allowed by modifying the system. The components of the sample will be separated on the basis of their ranging physical and chemical properties, imparting different affinities for the two phases.
We did this by putting two dots on a piece of filter paper and placing it in either water or methylated spirits and to observe what happened and to see if any of the groups matched the mystery inks colour pattern. The two major areas of the analytical sciences are Spectroscopy and Chromatography. Spectroscopy covers a wide range of techniques for chemical analysis at the atomic and molecular level. Chromatography is a general term for techniques that
Loss of C=C bond at third and fourth position increase the potency to 3-10 overlap. Substitution at position 4,5 or 8 with alkyl groups ordinarily lessens diuretic action and position 2 can tolerate a small alkyl group such as -CH3.4,5 The mechanism of action of thiazide is not fully understood but they act by inhibition of NaCl reabsorption in the cortical portion of the thick ascending limb of loop of Henle & Distal tubule and also inhibit electroneutral Na & Cl co-transport system. they are rapidly absorbed orally and have volume of distribution equal or greater than the body weight. They are strongly bound to plasma proteins so most of them are not metabolized but excreted as they are in urine as chlorothiazide and hydrochlorothiazide, but benzthiazide, bendroflumethazole and polythiazide are extensively metabolised.1,2The onset of most thiazides occurs after 2-3 hours. they have half life time nearly 8-12 hours permitting one dose per day.4,6 Thiazide diuretics were available at the end of 1950 when they had acceptable adverse effect.
However, the improvements were highly statistically significant in group B as compared to group A. This marked reduction in the proportions of sites that bled on probing represents significant reduction in inflammation and possibly, bacterial load in these sites. This is in agreement to another study by Gonzales et al17 where in the mean percentage of bleeding sites decreased from 95% to 35% after 10 days and to 42% after 28 days of placement of CHX chip. Goodson et al.18 have shown that local delivery of antimicrobial drugs had a significant effect in reducing the red-complex bacteria in the periodontal pocket. The larger reduction in PBI observed in the present study is likely due to the anti-infective effect that the frequent applications of these chips had on the microbial flora with chlorhexidine and inflammatory response due to flurbiprofen.