Adding 5% of ethanol to the slide enabled me to figure out how the Daphnia will act in that environment. One thing I had to make sure that it does not affect the experiment is the light in the microscope. I had to turn it off when the daphnia in on the slide to make sure that the heat doesn 't affect the Daphnia. Every two minutes I go to the microscope to check whether the Daphnia died or
The samples were filtered by vacuum over pre-weighed and wetted glass fiber filters (24 mm GF/C; Whatman; Kent, UK). The samples on the filters were rinsed with 5 ml stop solution to remove non-specific, cell surface binding of 3H-FLC. The filters with fungal balls were either allowed to dry for 24-48 hr or were baked in a drying oven for 15 minutes at 95° C. Each dried filter containing fungal balls was then re-weighed to obtain the dry mass of each fungal sample. The filters were finally transferred to 5 mL scintillation vials containing 3 ml of scintillation cocktail (Ecoscint XR, National Diagnostics, Atlanta GA). Radioactivity associated with the fungal sample on each filter was measured in a liquid scintillation analyzer (Beckman Coulter, LS 6500 multipurpose scintillation
We added 50ml of the buffer into the flask, and we boiled the solution for two minutes in the microwave. The sample well was taped with table and par film to keep the agarose gel from leaking. I loaded the 15ul of the five serum proteins: Cow serum, Serum albumin, Transferrin and Gamma globulins. The electrophoresed gel was placed on the voltage machine at 110 volts for 1.5hours. We removed the gel from the sample well and place the Coomassie Blue stain.
Next, 5 drops of Barium Hydroxide was placed in each tube to clear proteins and cell membranes for an accurate reading could be made. Blood Clots also interfere with the readings of glucose, so 1 drop of Heparin was placed in each tube to prevent blood clots. The tubes were mixed and centrifuged. After, the pellets were removed, 5 drops of Enzyme Color Reagent went into
The experiment began by setting up the LabQuest and preparing a 2M solution of HCl and a 2M solution of NaOH. This was called “Part A”. Two general rules were noted throughout the experiment: add acid to water and pour stock solution into beaker before graduated cylinder. This prevented flash-boiling of the solution, chemical burns, and spills. To make the 2M HCl solution, 200mL deionized water was added to a 600mL beaker labelled “2M HCl” by using a graduated cylinder.
In this lab, genes for a fluorescent green protein (GFP) and antibacterial resistance (ARG) were inserted into E. coli bacteria. E. coli bacteria was resuspended in an ice-cold CaCl2 solution. DNA containing GFP and ARG was added to half of the cells before they were “heat shocked” in an ice bath and hot water. The heat shocking made the bacteria’s cell membrane more porous, so the DNA could enter. Recovery broth was added to the cell suspension, and the bacteria was placed in warm water for about thirty minutes (see Results and Discussion, paragraph 2).
Worms A, B and C were then placed into separate containers containing the caffeine treatment solution. After allowing the worms to be treated for fifteen minutes, they were briefly rinsed and placed in the viewing chamber to measure their pulsation rate. This was done three times for each worm to calculate a mean rate for first level of treatment, 3.0 mM of caffeine. The same procedure was repeated for part II of the lab, however; a different set of three worms were treated with nicotine and all the means were collected to calculate the standard deviation of each
First, label one micro centrifuge tube +pGLO and another –pGLO. Using a sterile transfer pipet, transfer 250µl of competent cells (E. coli + CaCl2) into each tube and place them in crushed ice. Examine the pGLO plasmid DNA solution with the UV light and note your observations. Pipet 10µl of pGLO plasmid into the +pGLO tube and mix, close and return it to the ice rack. Do NOT add plasmid DNA to the –pGLO tube.
The Effect of Exercise on the Cell Continuity of Betta Splendens Keerthana Arjuna Joe Gibson George Washington High School Abstract The objective of ted this experiment was to observe how exercise would affect the cell continuity of betta splendens’ muscle cells. To conduct this experiment, three fish were put under three different amounts of exercise for fourteen days. After fourteen days, the fish were euthanized and tissue samples were obtained. The tissue samples were stained with acid fuchsin. After staining protocol, the samples were viewed through a microscope, using the highest magnification possible.
The removal method was done in the 10-meter section that was more upstream and capture-mark-recapture was done more downstream. The purpose of the removal method is to collect a sample of the crayfish and set it aside, away from anymore collections. The next capture should have relatively less than the first. This process is repeated until five trials are finished and each trial having less sample size than the previous one (Zippin 1958). The next method, capture-mark-recapture, is done by catching a sample in a bucket and recording the amount of crayfish and then releasing the samples back into the collection area once marked; in the specific experiment blue and pink nail polish were the marks used (Pradel 1996).