Fruit Fly Lab Report
Adewunmi Felicia Adebanjo
Principles of Genetics Lab report
The Fruit Flies: A Model Organism to Test for Genetic Equilibrium.
October 12th, 2017 Abstract
The traits studied in this lab are the presence or absence of wings, and eye color. Although these traits are not sex linked, the life cycle and sex determining markers for fruit flies was first identified and studied. The sex determining factors were used to differentiate the F 1 (male and female) before interbreeding the F1 generation. Using the observed traits of the parents, the genotypes of the F1 generation was derived. Using the observed phenotype and the derived genotype of the F1 generation, the phenotypic ratio of the F2 were hypothesized to be 9 winged and red , 3 wingless
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The bottom of the adult culture vial was tapped gently to settle the flies, while another culture vial is placed on top of the vial to catch the flies when they fly out. The two vials were then re capped to prevent the flies from escaping. A felt wand dipped in fly nap (an anesthesia capable of putting the flies to sleep for 45-60 minutes) is then gently inserted into the anesthetization vial to put the flies to sleep. When the flies appeared asleep, the sex determining features and phenotypes of parent 1, parent 2 were observed.
Anesthetization and mating of F1 generation
The F1 generation flies were also anesthetized using the above method, the traits of the males and females were observed. 7 red winged male and 7 red winged female, F1 generation flies were selected from the F1 generation. The medium for the next batch of flies was made by pouring 10 ml of formula 4-24 Instant Drosophila Medium to a clean vial and adding 10 ml of tap water, 4 grains of yeast and a strip of netting to crawl on when awake. The selected 14 fruit flies were transferred into prepared vials. The vials were marked and labeled and stored in an average temperature
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The two vials are then re capped to prevent the flies from escaping. A felt wand dipped in fly nap is then gently inserted into the anesthetization vial to put the F1 flies to sleep. After the F1 flies have been put to sleep, they were transferred into a vial containing ethanol to discard them. The breeding flies were left to breed for two weeks
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