Leah Romero 10/30/2017 Conclusion Lab 3 Chem 102L In lab 3, fundamentals of chromatography, the purpose was to examine how components of mixtures can be separated by taking advantage of different in physical properties. A huge process in this lab was paper chromatography, which was used to isolate food dyes that are found in different drink mixes. The different chromatograms of FD&C dyes were compared to identify which dyes are present in each of the mixes. Chromatograms where made for the known FD&C and for the three Kool-Aid samples. The retention factor for each dye was calculated.
How is cellulitis diagnosed? The diagnosis of cellulitis is suggested based on symptoms and physical examination – typical findings include spreading redness, warmth, pain, swelling, and fever. If you have a sinus tract that is draining pus, your doctor may obtain a sample and send it for gram stain and culture. This is also typically performed when you have an abscess that requires incision and drainage. The purpose of gram stain and culture is to guide antimicrobial therapy.
The samples were filtered by vacuum over pre-weighed and wetted glass fiber filters (24 mm GF/C; Whatman; Kent, UK). The samples on the filters were rinsed with 5 ml stop solution to remove non-specific, cell surface binding of 3H-FLC. The filters with fungal balls were either allowed to dry for 24-48 hr or were baked in a drying oven for 15 minutes at 95° C. Each dried filter containing fungal balls was then re-weighed to obtain the dry mass of each fungal sample. The filters were finally transferred to 5 mL scintillation vials containing 3 ml of scintillation cocktail (Ecoscint XR, National Diagnostics, Atlanta GA). Radioactivity associated with the fungal sample on each filter was measured in a liquid scintillation analyzer (Beckman Coulter, LS 6500 multipurpose scintillation
Fats, Soups, Sauces and Emulsions In my lab today I got to make some soup, biscuits, sauce, vanilla pudding, mayonnaise as well as oil and vinegar salad dressing. Emulsion is a process where a liquid is dispersed in another liquid with which it is usually immiscible. There are two types of emulsions. Oil-in-water (oil droplets are dispersed throughout the water) and water-in-oil (water droplets are dispersed throughout the oil). These emulsions can be permanent emulsion or temporary emulsion.
The materials that we used when conducting this experiment are: dissecting microscope, 3 petri dishes, C. elegans (male, female, and hermaphrodites), worm pick tool, agar, Bunsen burner, Parafilm, incubator, and flint spark lighter. The methods of this experiment are really simple. When we started the experiment we all washed our hands and wore gloves. Each group member did their part to conduct and successful experiment, one group member plugs the Bunsen burner to the gas pipe and turned on the gas, then used flint spark lighter to set the flame on the Bunsen burner. While the second group member is setting the dissecting microscope and making sure the lenses are clean.
Connect the spectrometer to Labquest and select a new file. Calibrate the spectrometer by placing the blank inside and allowing the lamp to warm up. The optimal wavelength for the standard curve and data collection can be started at this stage. Empty the blank and use the solution from test tube one to rinse the cuvette twice. Fill it ¾ with solution one, wipe the outside, and place it in the spectrometer.
The testing for affectability of a life form to antimicrobial agent is normally done utilizing agar dissemination or disk diffusion test. The parameters of this test were indicated (or institutionalized) by the researchers W. M. M. Kirby and A. W. Bauer and is likewise alluded to as the Kirby-Bauer antibiotic testing. In this technique, anti-toxins or antibiotic are impregnated on a specific extraordinary kind of paper circles and are put on the surface of agar containing the bacterium and parasitic (fungi) of our interest. This outcomes in the dispersion of antimicrobial agent into the surrounding medium. The diameter of the zone of inhibition will decide the adequacy or sensitivity of the antibiotic; the bigger the diameter, the more
(Holder, Robinson & Laub, 2011). In order to examine latent prints, I have chosen to conduct the experiment in the master bathroom. The different surfaces to be dusted for fingerprints include the glass shower door, the mirror door on the medicine cabinet, the toilet paper roll, the hardware on the linen closet, the bathtub faucet and the cardboard packaging for my face moisturizer. I have chosen to use Hershey’s Natural Unsweetened Cocoa powder and a BareMinerals Seamless Shaping and Finish makeup brush for this experiment, as they were handy. Additional supplies include Duck EZ start packaging tape and Mead index cards.
The other treatment for Raynaud’s syndrome is a chemical injection. The patient’s doctor will inject the affected area with Botox to block the nerves and cause the same results as the nerve surgery. This procedure is normally done in-office and may need to repeated if the patient’s symptoms do not dissipate. (National Institutes of Health, "How Is Raynaud 's
Recent guidelines for treatment of gonorrhea recommends the use of single dose injectable or oral cephalosporins. Monitoring and investigation of gonorrhea have become essential due to the emergence and spread of multi drug resistant and cephalosporin resistant strains of N. gonorrhoeae. Routine clinical laboratories need to be alert for the detection of such strain, so that plans for control and prevention could be reviewed and revised from time to time. It is essential to know the genetic mechanisms responsible for decreased susceptibility and future resistance. There is
With the spores on our toothpicks, we then smeared them onto a slide that was provided to us. We had distilled water that was lightly put on top of the smear to ensure that the cover slip would stick. After the cover slip was in placed we then got microscopes and set the stages and lighting to view the specimen. The spores were to be observed at a 4x magnification or 10X to see the color. We were to observe and record 30 asci and enter on computer spreadsheet, but asci with 8 spores of identical color were to
The patient has since returned to his primary care physician to find out what is going on. After Terrance visited with his PCP a sample was collected and necessary test were performed to determine what pathogen or bacteria was the cause of Terrance 's infection. In order to have a better understanding of what they are working with and how to