Essay On Nilotica

Plant Materials

The fresh leaves of A. nilotica were collected from the Foundation for Revitalization of Local Health Traditions, Bangalore, India (Latitude 12.9715987; Longitude 77.5945627).

Preparation of plant extracts

The fresh leaves of A. nilotica were collected, washed in tap water, shade dried for 10 days and made into a fine powder of 40mesh in size using the laboratory mill. 100g of powder was filled in the thimble and extracted successively with 70% of acetone, methanol, ethanol and chloroform taken on a clean flat-bottomed glass container and soaked for 72h. The container with its content was sealed and kept for a period of three days accompanying occasional shaking and stirring. The extracts were stored in sterile bottles

nilotica leaf extracts (0.3 mL) were mixed with 2.7 mL of methanol solution containing DPPH radicals (6×10-5 mol/L). The mixture has shaken vigorously and allowed to stand for 60 min in the dark. The reduction of the DPPH radical was determined by the absorbance at 517 nm (Barros et al. 2007). The radical-scavenging activity (RSA) was calculated as a percentage of DPPH discoloration, using the equation: %RSA = [(ADPPH - AS) /ADPPH] ×100, where, AS is the absorbance of the solution when the sample extract is added at a particular level and ADPPH is the absorbance of the DPPH solution. The extract concentration is providing 50% of radical-scavenging activity (IC50) was calculated from the graph of radical scavenging activity RSA percentage against extract concentration. Ascorbic acid was used as

marmelose was prepared in dimethylsulfoxide (DMSO). Different concentration of (25, 50, 75 and 100 µg/ml) of extract was prepared in cell culture medium before use.

3-(4, 5-dimethylthiazolyl-2)-2, 5-diphenyltetrazolium bromide (MTT) cell proliferation assay

MDA-MB-231, HEp-2 and Vero cells (1X105/well) were seeded in 96 well plate (100 µl/well) and allowed to adhere firmly overnight in DMEM, MEM and RPMI 1640 medium respectively with 10% FBS. Then cells were treated with different concentration of freshly prepared extracts for 24h. Then medium was removed and cells were incubated with MTT reagent (5mg/ml) for 4h and violet crystals dissolved in DMSO and absorbance was read at 540/690 nm. Absorbance of control (without treatment) was considered as 100% cell survival. Doxorubicin was used as positive control.

Statistical evaluation

Data presented as mean ± SD of two duplicates of three independent experiments. Experimental data were evaluated by students‘t’ test and one or two way analysis of variance (ANOVA). Significant difference between each set of data were considered at the confidence level of