Cell Viability Test Lab Report

The investigation was carried out to identify the presence or absence of biological molecules in serum 2216. If the concentration in each test tube of the dilutions carried out will be more concentrated then the concentration of the test tube before it, then the color will be at an equal concentration with the other dilutions performed. The hypothesis was wrong because of the difference in concentrations due to the different measurements within the dilutions done. The test for starch was to add a drop of iodine solution to the pipette in the spotting tile. A reducing sugar solutions is add inside a test tube with 3 drops to then add 3 drops of benedicts and plane in a water bath.

Problem 3 solutions using different approaches in the literature Approach Reference Machine cells Number of intercellular

These cells have been the oldest and most commonly used human cell line, and it is also remarkably durable and prolific

Third, a series of stains/solution such as crystal violet, iodine, ethanol, and Safranin were used to complete the Gram Stain. During this process each stain/solution was left on the emulsion for a limited amount of time. Also between each stain, there was a removal of the previous stain by rinsing the slide clear with distilled water. After the gram stain was completed, the slide was blotted dry with bibulous paper and was placed under the microscope. While under the microscope, a drop of oil

LABORATORY REPORT EXERCISE #5 INTRODUCTION TO THE COMPOUND LIGHT MICROSCOPE, PLANT AND ANIMAL CELLS Name_______________________________Section_____Teacher______________Date________ PRE-LAB QUESTIONS - answer the following questions using your textbook and valid internet sources. Be sure to cite your sources at the end of the prelab. You can type your answers to all questions except #1 and #9 directly into this document and then submit via Canvas. Type the answers for #1 and #9 at the end of the document. 1.

After 4 hours, the culture medium was removed and cells were treated with 200 μL of nanoparticles containing an amount of 5 μg of siRNA-TNF-  plus 200 μL of incomplete AMEM medium added without FBS and PS. Cells treated with 200 μL of free siRNA were used as a negative control. After 3 hours of incubation at 37 °C, the medium was removed and the cells received 1 ml of complete medium with 10 % FBS and 1 % PS and incubated for 48 hours. After this time, the medium was removed and a new complete medium containing 100 ng / ml lipopolysaccharide (LPS) was added [30]. After 24 hours, the medium was removed and stored for TNF-α protein concentration analysis using the Elisa quantikine immunoassay kit for mouse TNF-α (R & D Systems, Minneapolis, USA ), following the manufacturer's instructions.

WARD’S SIMULATED BLOOD Objective: The objective of this lab is to use Ward’s simulated blood to know the blood types and Rh factor of four people. Hypothesis: Based on the exercise, if Mr. Smith is a blood type A+, then he will coagulate with anti A and anti Rh. If Mr jones is a blood type B-, then he will coagulate with Anti- B serum. If Mr Green is a AB+, then he will coagulate with Anti- A, B and Rh.

Starch solution is then placed into the test tube at a quantity of 5 mL. 5 drops of Lugol’s Iodine solution is added to the test tube. If the color changes, then it is known that starches are present in the solution. Proteins are next tested. In order to do this, 5 mL of gelatin solution is added to the test tube. 10 drops of Biuret’s reagent are added to test for protein.

This root tip was choosen because of its rapid growth and it can be easily avaliable and grown in large numbers. The rapid root growth proved advantageous as it allowed the observation of multiple cells in each mitotic stage within a small sample. It was expected that the majority of the cells found would be in interphase as a large proportion of the cell division cycle is spent with the cell performing its normal cellular functions. Materials: The Materials required for this experiment include; a

They are not exposed to as many outside factors as epithelial cells, in skin cells, therefore not dividing as much as when needed. The Allium Root Tip cell average mitotic index, (the number of cells going through mitosis compared to the total number of cells), was greater than human bone cells. The way different cells react to mitosis, and stage length

Additionally, mitotic spindle disintegrates and chromosomes are uncoiled into chromatin, returning the cell to the interphase structure. Even though mitosis is finished with telophase, the final cell segregation occurs at cytokinesis. It starts before telophase and divides the cytoplasm of the cell, therefore making two daughter cells with the genetically identical nucleus. The aim of the practical was to observe the sample of the onion root tip under the microscope and to count cells in different mitotic phases.

Demonstration of Cellular Respiration of a celery cell in vivo. Purpose Cellular respiration is a metabolic process consisting of a series of oxidation reactions in which oxygen is utilized and therefore is called an aerobic reaction. The process of cellular respiration takes place in the mitochondria, located in the cell of an organism and which converts biochemical energy from nutrients into adenosine triphosphate (ATP), and then releases waste products. The mitochondria is surrounded by two membranes, the inner membrane and outer membrane.

In this lab when looking at cells, we observed the salinity and osmolarity of the cell when placed in the environment. With the different concentrations of NaCl, we are able to see how different environment can constrain an organism and see the wide range of responses to regulate in cell’s osmolarity. The cells we studied was sheep red blood cells (erythrocyte), because they are the most studied membrane system and therefore used as ideal membrane to study the relationship between water and the passing of the different concentration of NaCl across the membrane. The purpose of the experiment was to observe the cell and infer which direction of the flow of the water due to the cell volume change.

→ Calculation of the absolute values of the different leukocytes in the differential was accomplished via the following formula: (total WBC count x relative value in %)/100 On the chemistry tests: increased uric acid (8.4 with normal being 4-6 mg/dL) increased lactate dehydrogenase (LD) (692 with normal being 140-280

However, cancer treatments nowadays are very risky and have severe side effects such as blood clotting, bone issues, and hair loss. More efforts should be put in the deeper understanding of glycolysis and other pathway. In addition, it would me more efficient to find other treatments with less side effects that target the stage of glycolysis in tumor cells, inhibiting the overexpressing of enzymes and the production of lactic, leading to stop the abnormal cell division, and cure