Cell Viability Test Lab Report

1000 Words4 Pages
Experiment 3: Cell Viability Testing and Counting with Trypan Blue Exclusion Method
Anna Coretta R. Santos

I. Introduction

Cell viability assay plays a crucial role is in the cells of the cell culture. This assay can be used to identify the relationship of cell count from the cell’s behavior (Stoddart 2011). This test help researchers to determine the number of viable and non-viable cells in a cell culture. This assay helps in estimating the cell count of a cell culture and to determine if there is sufficient amount of viable cells in the cell culture that a person will use for future research (Riss et al. 2013).

A vital tool used in cell viability testing is a hemocytometer. This counting chamber is used for counting cells and helps researchers
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Conclusion and Recommendations

Ultimately, testing cell viability and knowing the count of viable and non-viable cells in a cell line is an important key in any research using cells. Having enough number of viable cells in a suspension would give accurate results in an experiment. This also shows how trypan blue is an important dye in cell viability and in the study of cells. This dye makes researcher’s life convenient in identifying and differentiating viable versus non-viable cells or live versus dead cells.

It is really needed that researchers should ensure new and fresh cell cultures to ensure more viable cells in a culture. It also better to ensure that the materials like hemocytometer and pipettes are sterilized and clean so that there would be lesser or no artifacts would be seen under the microscope. The researcher was to use trypan blue exclusion method to test for cell viability, observe the non-viable and viable cells, and was able to have a cell count using the
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[Internet]. [Updated: 2012 Aug 10]. Houston: Rice University. [cited 2017 Feb 4]. Available from http://www.ruf.rice.edu/~bioslabs/methods/microscopy/cellcounting.html

Riss, T., Moravec, R., Niles, A., Duellman, S., Benink, H., Worzella, T., Minor, L. 2013. [Internet]. Cell Viability Assays. Bethesda (MD): Eli Lilly & Company and the National Center for Advancing Translational Sciences. [cited 2017 Feb 4]. Available from https://www.ncbi.nlm.nih.gov/books/NBK144065/

Stoddart, M. 2011. Cell viability assay: Introduction. Methods in Molecular Biology 740: 1-6.

ThermoFisher Scientific. [Internet]. Masasachusetts: ThermoFisher Scientific. 2016 Dec- [cited 2017 Feb 2]. Available from https://www.thermofisher.com/ph/en/home/life-science/cell-analysis/flow-cytometry/cell-health-and-viability-assays-for-flow-cytometry/cell-viability-assays-for-flow-cytometry.html

Tran, S., Puhar, A., Camus, M., Ramarao, N. 2011. Trypan Blue Dye Enters The Viable Cells Incubated with the Pore-Forming Toxin Hlyll of Bacillus cereus. PLoS One 6(9).

Yip, D., Auersperg, N. 1972. The dye exclusion test for cell viability: Persistence of Differential staining following fixation. In Vitro 7(5): 323-329.

V. Answer to Guide
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