blood cells are placed into a hypotonic solution of distilled water, then the cells will undergo a process called hemolysis where they swell and burst. For this experiment, the null hypothesis is that if blood cells are placed into a hypotonic solution of distilled water, then the cell will not undergo hemolysis. For the experiment involving the elodea plant, the hypothesis is that if the leaf of an Elodea plant is placed into distilled water, then the cells will become turgid, but will not burst because
concentrations of water-soluble salts, mainly NaCl. Salinity is usually caused by two mechanisms: groundwater salinity and irrigation salinity. Groundwater salinity occurs when saline groundwater is present in the upper layers of the soil. This commonly occurs in areas where native vegetation has been cleared and evaporation rates are high, like the West Australian wheat belt. Irrigation salinity occurs when irrigation water accumulates in the upper layers of soil. When this water evaporates, the salts
Objective The purpose of this lab was to demonstrate and view the osmotic process without using a microscope or chemical testing. In order to do so, background knowledge on the direction and flow of water is needed to identify the movement of osmosis. Hypothesis The hypotonic solution will cause the potato strip to become heavier relative to its previous mass, the hypertonic solution will cause a decrease in the potato strip’s mass, and the isotonic solution will result in no change in mass.
assigned 25% saturation. 3.33mL of the liquid ammonium sulfate was added to 10mL of the protein extract (given by the instructor) in a centrifuge tube to precipitate the protein out. Water was then added to a second centrifuge tube to balance out the mass in the centrifuge. The mass of the mixture was weighed and the water was measured (using a scale) to weigh the same as the mixture. Both centrifuge tubes were place in the centrifuge for ten minutes at 12,000rpm. The supernatant was pipetted out of
NaOH was slowly added to the “tap water with Elodea” beaker with a dropper that delivers 0.025 mL per drop. Each drop was counted and the solution was stirred thoroughly before adding the next drop. This was continued till a faint pink color appeared in the solution. The number of drops was noted. The procedure, from the addition of NaOH to the noting of number of drops, was repeated for the other three beakers. The number of drops for the beaker labelled “tap water” was subtracted from the number
the temperature of perfusate reservoirs, perfusate lines, and saline bath are at 10oC. 3. Turn on the circulation valves for chiller 1 and turn on the bypass valves for chiller 2 4. Set up the inline perfusate delivery at the constant pressure of 0 cmH2O and outflow line at 30 cmH2O. 5. Ensure that perfusate is flowing through the lines adequately 6. Clamp off the outflow line Transfer 7. Transfer and fully immersed the fish in the saline bath of constant temperature 10oC 8. Connect the input cannula
the yolk was put on a filter paper to roll in it to eliminate completely the remainders of the white adhered. The yolk membrane was punctured and the content of the yolk (approximately 15 ml) was deposited in a test tube. An equivalent volume of saline solution was added (NaCl 0.16 M; pH = 7) and it was mixed completely. The resultant solution was centrifugated for 30 minutes at 9000 rpm at 4ºC in a rotor Beckman JA-20, eliminating the pellet. The supernatant was collected to obtain the lipoproteins
prepared in clean and dry, wide mouthed glass vials to which 400µL of absolute alcohol was added and after confirming the formation of homogenous dispersion, the glass vials were heated thermostatically on a water bath at 60-70oC with intermittent shaking. Add 160µL of double distilled water maintained at the same temperature to the transparent solutions formed, these upon cooling change to yellow translucent liquid/gel or white creamy proliposomal gel. Proliposomal gel formulations with positive
Josie Krueger March 22, 2023 Title: An Inquiry into the Effect of the Molarity of Salt Water on the Boiling Point Word Count: 984 Overview: This lab was an inquiry into the relationship between the molarity of saltwater and its boiling point. Testing in this lab consisted of four separate molarities, along with three trials per molarity. The lab modeled a form of chemical reaction called ionic bonds, and highlighted common chemical reactions in life. Testable Question: How does the
the solid thus obtained was filtered, washed with cold water and recrystallized with ethanol to obtain the compound 4. 2.4. General procedure for synthesis of 1-{(5-substituted-1,3,4-oxadiazol-2-yl)methyl}-2-(morpholinomethyl)-1H-benzimidazoles (5a-r) An equimolar mixture of compound 4 (0.001 M) and substituted carboxylic acid in phosphoryl chloride (POCl3) was refluxed for 8–12 h. Then reaction mixture was cooled, poured into ice-cold water and neutralized with 20% w/v NaHCO3 solution.
ALT, and ALP was purchased from ----------. All the others reagents use are of high purity and of analytical grade. 2. Animals: BALB/C mice, 15-20 g were housed in colony rooms with 12/12 hr light/dark cycle at 21±2°C and had free access to food and water. All animal experiments were carried out in accordance with CPCSEA guidelines, Ethical committee Acts. 3. Experimental protocol: Animals were acclimatized for a period of 1 week prior to the experiment. Later they were divided in to five groups containing
Sea is the largest lake in California. It covers approximately 343 square miles and has a maximum depth of only 43 feet. The Salton Sea is a salt water lake that was created by accident, when the salt flat known as the Salton Sink was flooded. In the year 1905, heavy rainfall caused several canals off of the Colorado River to swell, which caused water to flood into the Salton Sink, creating the Salton Sea. For a time, the Salton Sea was a popular tourist attraction with many resort towns around the
on diffusion in yam cores compared to the solution concentration of water Abstract The purpose of the experiment was to see if different solution concentrations had an effect on diffusion. Our group established a hypothesis that stated; sodium chloride will make the yam cores weigh less than in water. In order to start experimenting, we obtained 10 yam cores, weighed them and placed them in five cups that contained 50 mL of water. At 10-minute intervals, we would take them out and weigh them again
rooted plants; shallow rooted plants can’t keep the water table sable as they aren’t native. Salinity impacts farms, rivers, drinking water, buildings and roads, vegetation etc. The Murray Darling Basin is a major source for civilization as 3 million people use the Murray Darling Basin for house-hold needs. Salinity is the amount of salt present in the land’s surface, or dissolved in water in rivers or ground water. Salinity is caused by the water table rising which causes the salt to rise to the surface
capacity in barrels of produced water. The steps to do the calculation of the upper section of the Birds Nest aquifer is the following:
the Indian ocean and the Red sea. Its natural habitats are subtropical or tropical mangrove, open seas, shallow seas, subtidal aquatic beds, coral reefs, estuarine waters, intertidal flats, coastal saline lagoons. It is threatened by habitat loss and over fishing. The predatory fish reaches upto 1.2m (3.9 ft) in length. The waters of Dubai are home to more than 300 species of fish, including the hamour. The groupers are resident hunters on many reefs and include among their numbers,
classes. Such extremophiles are called polyextremophiles. The common categories of extremophiles are as follows: Psychrophiles Organisms with the ability to survive at temperatures below –4° F. Temperature plays havoc at extremely low temperatures. When water freezes and ice crystals are formed in the cell, the crystals and rupture the cells- killing it in the process. They produce unique proteins that act as antifreeze agents and protect them against freezing. Some have layers encompassing the cell which
Mollenhauer Life Science 11 December 2017 Hydrosera Whampoensis Report The protist I chose was the diatom Hydrosera Whampoensis. I chose this diatom because of its interesting shape. Diatoms are protists that live in water. Different species of diatoms live in different types of water. Diatoms are photosynthetic, which means they make their own food in the same way that plants do. Most diatoms have golden-brown pigment in them. The cell walls of diatoms are made of silica. Silica is, in essence, glass
using phosphate-buffered saline (PBS). The washed packed RBCs had a hematocrit of about 80%. RBCs loading with FVIII Stepwise hypotonic dialysis was used to load factor FVIII (Lyophilized powder from human plasma produced by Biotest, IBRF) in RBCs. Two ml of washed pack cells and 250 unit of FVIII were put into a dialysis bag (Sigma). Dialysis bag presoaked for 4 h in 50 ml PBS buffer containing 5 mM glucose. The bag was placed in a
that were designed to protect residents and property adjacent to the river, but blocked spring flood water that would otherwise bring fresh water and sediment to many area of marshes. Swamps have been extensively logged, leaving canals and ditches that otherwise allow saline water to move inland and destroy landscape. Canals that are dug for the oil and gas industry also allow storms to move sea water inland, where it has damaged swamps and marshes. Rising sea levels also attributed to global warming