Spectroscopy Essays

  • Spectroscopy Lab Report

    846 Words  | 4 Pages

    Index 1. Introduction Spectroscopy is a term which refers to the interactions of various types of electromagnetic radiation with matter. It is also a study of the absorption and emission of light and other radiation by matter. Dependence of this process related to the wavelength of the radiation. Spectrometers are used to measure the properties of light over a specific portion of the electromagnetic spectrum. Fig 1: Dispertion

  • Spectroscopy: Electromagnetic Radiation

    1076 Words  | 5 Pages

    Spectroscopy deals with the production, measurement and interpretation of spectra due to interaction of electromagnetic radiation with matter which is absorbed or emitted by atoms of a sample. This absorption or emission occurs when the atoms of the sample move from one energy level to another in presence of light. In other words, it is a science which deals how light interacts with matter. When atoms or molecules absorb electromagnetic energy, the incoming energy promotes the molecular system to

  • Beta Carotene Research Paper

    804 Words  | 4 Pages

    3. Identification of Beta carotenes. Focus of this review is the identification of beta carotenes in the infrared spectrum region. In terms of metabolism and potential effects on health, beta carotene is one of the examples of most studied carotenoids. Thus the basic structure of beta carotene is made up of isoprene units. These isoprene units are joined end-to-end to give a conjugated chain which is common to all carotenoids. It is cyclised at each end which will looks like in the Figure 7. The

  • Experiment Stock Benzoic Acid

    717 Words  | 3 Pages

    placed in a 100-mL volumetric flask along with 10.0-mL 0.10m HCl and diluted. Then, the UV absorbance of the five standards was taken. Water was used in the reference cuvet. The wavelength of the peak absorbance for caffeine was also taken. The spectroscopy machine automatically corrected the absorbance values against the baseline of the water reference. The results were graphed to create an absorbance versus molar concentration calibration curve. Finally, the UV absorption of the soda sample was

  • Circular Dichroism

    994 Words  | 4 Pages

    Introduction Circular dichroism (CD) is form of light absorption spectroscopy that measures the difference in absorbance of right- and left-circularly polarized light (rather than the commonly used absorbance of isotropic light) by a substance. It is applicable for molecules have one or more chiral chromophores [1]. Circular dichroism = ΔA(λ) = A(λ)LCPL - A(λ)RCPL, where λ is the wavelength This technique measured a molecule over a range of wavelengths. All chiral molecules can be studied, particularly

  • Column Chromatography Essay

    1137 Words  | 5 Pages

    Detection and Purification A Monascus pigments is a complex of azaphilone compounds, which can be separated by using various analytical techniques. UV- Visible spectrophotometric methods The UV-Visible spectrophotometric method is usually used for the confirmation of pigments produced by Monascus with taking absorbance at a respective wavelength. i.e. Yellow pigment at 400, Orange 470, and Red at 500 nm. The pure solvent or extract of unfermented substrate was always used as the blank (Carels and

  • Tetraphenylnaphthalene Lab Report

    779 Words  | 4 Pages

    and percent yield was calculated (65.5%) with the limiting reagent being tetraphenylcyclopentadienone. The melting point was determined. The first melting point was 204-204.9 °C and the second melting point was 215.6-215.9°C. Finally, an infrared spectroscopy was obtained for the

  • Spectrophotometry Essay

    932 Words  | 4 Pages

    1.1. UV-SPECTROPHOTOMETRY Spectroscopy is the measurement and interpretation of electromagnetic radiation absorbed or emitted when the molecules or atoms or ions of a sample move from one energy state to another energy state. Spectroscopy is a general methodology that can be adapted in many ways to extract the information you need (energies of electronic, vibrational, rotational states, structure and symmetry of molecules, dynamic information). Ultraviolet-Visible Spectrophotometry is one of

  • Genotoxic Alkyl Sulfonates Lab Report

    1654 Words  | 7 Pages

    secondary sulfonate isomers. The analysis of each isomer is important for the elucidation of reaction mechanism of sulfoxidation and their physical properties. The authors carried out the isomer analysis of PS by means of Nuclear magnetic resonance spectroscopy (NMR) method with the NMR shift reagent, Eu (dpm). In the ordinary NMR spectrum of methyl alkyl sulfonate, the information on the position of sulfonicgroup could not be obtained. It was found that methyl signals of ester group shift toward lower

  • Molybdenium Oxide Lab Report

    1362 Words  | 6 Pages

    Figure ( 1 ) shows the XRD for bismuth borate glass samples containing molybdenium oxide from 0 to 20 mol %. From figure (1) can observe that the samples containing molybdenium oxide up to 15mol%MoO3 are in the amorphous state. Then the sample containing 20mol% MoO3 are I the amorphous and crystalline state. The analysis of the sample containing 20mol% MoO3 poured between two copper plates or poured at air, the x-ray diffraction patterns indicate the formation of Bi2MoO6 crystalline phase formed

  • Spectrophotometry Lab Report

    897 Words  | 4 Pages

    Spectrophotometry Prepared for: Dr. Joseph Dasso By: Lucy Onsarigo Biology 1406 C5L September 23rd, 2014 Introduction Spectophotometry is the ability of molecules to absorb and transmit light energy for determining the concentration of substances in a solution. (Mark Garcia 2014). The instrument used is called spectrophotometer to distinguish different compounds since they absorb light at different wavelength. Some have wide range of wavelength and the shorter the wavelength the higher

  • Spectrophotometric Analysis Of Dyes

    904 Words  | 4 Pages

    Introduction: The objective of this experiment was to use a spectrophotometer to calculate the absorbance wavelength for the commercial dyes that were given. Light is composed of tiny particles that are called photons, just like matter is composed of tiny particles called atoms. Using the spectrophotometer you can see that different dyes absorb at different wavelengths. With all of the experimentation done the concentration, absorbance and the max wavelengths should be found. Figure 1: Schematic

  • Colorimeter Lab Report

    1015 Words  | 5 Pages

    Colorimetric method –instrument and application Introduction: - Colorimeter is device which is used to measure transmittance and absorbance of light passing through a liquid sample. It is a light sensitive device. Principle: - Colorimeter works on the principle of Beer-Lambert’s Law. Beer-Lambert’s Law states that when visible light from a natural or artificial source falls on a colored homogenous medium contained in a glass container, the color of the medium is the complementary color of the light

  • Iron Phostate Lab Report

    1052 Words  | 5 Pages

    Iron phostate, also extensively known as FePO4, was studied through a special type of transformation process known as neutron powder diffraction. The transformation process uses a temperature that ranges from 297K to 1073K. As long as it is within the low temperature range, it is defined as the ‘α’ phase. Otherwise, if it is within the high temperature range, it is defined as the ‘β’ phase. The iron phostate will change its tetrahedral form in the low temperature range to its octahedral form in high

  • Atomic Absorption Spectroscopy

    649 Words  | 3 Pages

    Typically, modern chemists make use of Atomic Absorption Spectroscopy to determine, quantitatively, the concentration of small and often trace elements found within certain substances and/or the environment. such measurements can be made due to the sensitive nature of AAS, as measurements that are taken can be into the parts per million (ppm). In principle, AAS works, by using ground state atoms. (which are atoms that have the lowest possible shell orbit to the nucleus due to containing very little

  • Nmr Spectroscopy Lab Report

    1580 Words  | 7 Pages

    Introduction of NMR spectroscopy Nuclear Magnetic Resonance (NMR) spectroscopy is a form of absorption spectroscopy by which a nuclei under investigation absorbs the energy from an external magnetic field and resonances or excites from a low energy state to a high energy state. It involves the disintegration of spin state into two spin states of unequal energy. The emerging radiofrequency is absorbed in a magnetic field resulting to the magnetic properties of the nuclei which getting up from the

  • Antir Spectroscopy Lab Report

    1115 Words  | 5 Pages

    FTIR Spectroscopic Study on Quantitation of Urea in Human BloodSerum Abstract: The quantitation of urea has been achieved using FTIR spectroscopy. The FTIR spectra of human blood serum samples are recorded in Mid IR region 4000-400 cm-1.The normal blood serum is treated with urea at different concentrations and FTIR spectra are recorded, which confirm the specific peaks related to urea. A plot between concentration of urea and percentage of absorbancehas shown linear relationship. The study being

  • Ir Spectroscopy Lab Report

    851 Words  | 4 Pages

    Distillation and IR Spectroscopy CHE 361L Christian Johnson 02/17/2018 Introduction The purpose of this lab was to separate an unknown binary mixture by distillation and use IR spectroscopy along with the placement of known functional groups in order to determine the identity of the compounds. Based upon the potential unknown solutions, there are a few specific functional groups that can specifically be targeted in order to accurately depict the identity. The functional groups and specified area

  • Nmr Spectroscopy Advantages And Disadvantages

    879 Words  | 4 Pages

    NMR (nuclear method resonance) spectroscopy is the method of choice for the investigation of complex fluid mixtures with analytically similar compounds, where other analytical methods (e.g., optical spectroscopy such as UV/ VIS , infrared (IR), Raman, or fluorescence spectroscopy) suffer from insufficient differentiation of components. In addition, the high value of NMR in determining chemical structure and accurate quantitation, more subtle features such as speciation (e.g., protonation) are clearly

  • Bohr And Heisenberg's Uncertainty Theory

    1749 Words  | 7 Pages

    The Uncertainty principle In 1927, Werner Heisenberg was working at Bohr’s research institute in Copenhagen, Denmark. Neil Bohr and Heisenberg were working closely together on theoretical investigations of quantum theory and nature of physics. Heisenberg was left back at the centre alone when Bohr was away skiing. At this point, Heisenberg realized the limits of physics and physical reality. He realized that it in the act of observing, the observer somehow, manages to alter the reality. This observation