The cells were maintained in Minimal Essential Media supplemented with 10% FBS, penicillin (100 U/ml), and streptomycin (100 μg/ml) in a humidified atmosphere of 50 μg/ml CO2 at 37 °C. Reagents: MEM was purchased from Hi Media Laboratories Fetal bovine serum (FBS) was purchased from Cistron laboratories Trypsin, methylthiazolyl diphenyl- tetrazolium bromide (MTT), and Dimethyl sulfoxide (DMSO) were purchased from (Sisco research laboratory chemicals Mumbai). All of other chemicals and reagents were obtained from Sigma Aldrich Mumbai. In vitro assay for anticancer activity (MTT assay). The anticancer activity of samples on HepG2 was determined by the MTT assay (Mosmann 1983).
The Glycemic index uses a scale of 0 to 100 with the higher values of the food being the one that causes the rapid rise in the blood glucose level. These figures are done by several tests by taking samples of one’s blood after one has eaten. The label on foods that read “Low GI” shows one that the food will take longer to absorb into the body as well as to break down into the blood stream making one feel fuller for longer. Although eating
Methotrexate inhibits this enzyme hence NADPH, which was recorded spectrophotometrically. These assays have been well described for determination of Serum concentrations. In the 1985 the Schroder and co-workers studied the same enzymatic assay but they use erythrocytes as sample. To study the clinical relevance of erythrocyte methotrexate this enzymatic method
Background: Alfalfa (Medicago sativa) is a leguminous plant with high contents of phytoestrogen and saponin which are both useful in preventing diabetes disorders. Diabetes is a common metabolic disorder that is specified by hyperglycemia resulting from defects in insulin secretion, insulin action, or both. The aim of this study was to investigate the effect of aqueous extract of alfalfa on blood glucose and serum lipids in alloxan diabetic rats. Method: Thirty-two adult female wistar rats weighing 210-250 g were recruited and divided randomly into four groups of eight animals each for 21 days as follows. Control group, diabetic control group, diabetic group + aqueous extract of alfalfa (250mg/l), diabetic group+aqueous extract of alfalfa (500mg/l.
Conclusion It was observed that commercial headache powder contained the active ingredient (paracetamol), as melting points of both crude and purified samples were in the range values of melting points of crude and pure paracetamol in the literature, for instance, 160.8 to 176∁.6 and 169 to 171℃ respectively(Prybyl et al. 2013). Experimental errors might have occurred during the experiment had insignificant effects on the precision of the experiment since the results obtained were within the theoretical values of
The reported melting point for acetanilide is 114.3°C, meaning that my range provides support that the product obtained was what was desired. Next, a TLC plate was run to evaluate if the reaction was successful. I spotted three lanes on my plate, diluted pure aniline, diluted pure acetanilide product, and a co-spot of them both for reference. Ethyl acetate was used to dilute the sample to attempt to minimize streaking and I used a developing solvent of 80% ethyl acetate and 20% hexane. There was no starting material present in the product lane, meaning the reaction ran to completion.
In this study, they examined the blood levels of renalase in patients with chronic renal disease and decreased renal function. Renalese was undetectable in their blood. Xu J et al. (1) 2) Animal trials Wild type (n=11) Knock out (n=9) It was found out that renalase deficiency aggravates ischemic myocardial damage. The degree of myocardial necrosis was increased in renalase KO hearts Wu Y et al.
Drug profile of doxorubicin Doxorubicin (DOX) also known as Adriamycin is categorized as anthracycline antiobiotic. It was first extracted from Streptomyces peucetius var. caesius by aerobic fermentation followed by solvent extraction and purification by chromatography in 1970s. It is also extracted from Streptomyces coeruleorubidus or chemically synthesized from daunomycin (its natural analog). DOX differes from daunorubicin only by a single OH group (Arcamone et al., 1972).
1. Introduction Hepcidin is a peptide hormone synthesized mainly in the liver and the active form consists of 25 aminoacids. Some studies have demonstrated the relationship between hepcidin and iron. Nicolas et al. observed a severe iron overload in mice that were knockout for the gene encoding hepcidin .
The extraction was done by blending 10g of fish with 270ml of buffer solution to homogenized solution. The homogenized solution was centrifuged at 3000 rpm for 15 minutes. The supernatant was filtered and tested with Biuret reagent. Both fish shows positive result of biuret test with the presence of protein. From the results of spectrophotometer, the absorbance of tilapia at 570 nm is higher than the absorbance of mackerel, which is 0.100 and 0.094 respectively.