Essay On Vitex Negundo, Emblica Officinalis

The leaves of Vitex negundo, Emblica officinalis and Tridax procumbens were collected from Kem village, Solapur district of Maharashtra during Monsoon season. The herbarium was made and authentication was done from Botanical Survey of India under the code
TAY3.BSI/WRC/Tech/2011.The voucher herbarium specimen was submitted to the APT
Research Foundation, Pune, India.
The preliminary phytochemical screening of Vitex negundo, Emblica officinalis and Tridax procumbens showed the presence of different organic elements which are shown in
Table 1.
Saponins were absent in the aqueous extract of Vitex negundo whearas present in ethanolic extract, Also Alkaloid, Carbohydrate, Flavonoids, Tannins, Phenols, Saponins and
Phytosterol were present

In the present study, the total tannin content was estimated and represented in the figure 16. The total tannin content was estimated by comparing with standard curve of
Catechin.(Figure 15) It was found that the aqueous extract of Vitex negundo contained 278.6 ±
3.8 mg/gm and ethanolic extract contained 230.9 ± 1.3 mg/gm. Whereas, aqueous extract of
Emblica officinalis contained 311.9 ± 5.2 mg/gm and ethanolic extract contained 286.1±6.3 mg/gm. The aqueous extract of Tridax procumbens contained 246.9 ± 2.9 and ethanolic extraccontained 253.6 ± 3.8. The aqueous extract formulation of three plants contained 366.9 ± 1.4 and ethanolic extract contained 359.9 ± 0.5 dried extract equivalent to Standard Catechin (Table 4)
[R
2 value 0.992]Aqueous as well as ethanolic extracts of Vitex negundo leaves, Emblica officinalis bark and Tridax procumbens plant for its free radical scavenging and antioxidant property were screened by using ABTS assay. Aqueous and ethanolic extracts were found to scavenge the superoxides generated by photoreduction of riboflavin in dose dependent manner. The

The aqueous extract of VN and EO showed a significant reduction in the paw edema volume upto 2 hrs after Carrageenan injection. While ethanolic extract showed comparatively much less activity (Figure 18 and Figure 19). Aqueous extract of TP was more effective than ethanolic extract. Both the extracts had showed significant anti-inflammatory activity (Figure 20). The formulation made up of the aqueous extracts of three plants also showed significant reduction of the paw volume which is quite similar to the standard drug
Indomethacin (10 mg/kg) treated group up to 2 hrs. (Figure 21)Whereas formulation made up oThe effect of wound healing activities of the plant extracts were evaluated in incision wound model and in terms of skin breaking strength and converted to tensile strength as given in Table 13. Control group, standard drug and test extract treated groups were then compared statistically. The aqueous extract of VN showed 41.0 gm/mm
2
and EO 42.3 gm/mm
2
of tensile strength, and found to be statistically significant (P<0.05) when compared with the control. Standard drug treated groups showed 31.0 ± 5.6 and 39.7 ± 3.7 gm/mm
2
of tensile strength. While, ethanolic extract of TP showed 41.7 ± 9.2 g/mm