The mixture is then distilled to separate the product. A reflux apparatus: A distillation apparatus: Preparation and testing of an organic liquid Preparing and testing an organic liquid for purification has 3 distinct parts to it: Reflux, Separation and Distillation. Reflux and Distillation reactions must be carried out in a fume cupboard to avoid toxic vapours from escaping and must also be allowed time to boil and reflux/distil. Safety measures:
The resin that had stuck to the sides of the burette was washed down by pipetting extra pH 3 citrate buffer along the sides. The column was tapped to ensure that the settled resin formed a level surface. After all of the resin settled, the buffer was drained into a waster beaker until the level of the buffer reached the top surface of the resin. For the remainder of the experiment, the top surface of the resin was not allowed to dry
Solvent n-hexane, ethyl acetate and acetone will use to elute the column in isolation of chemical constituents of figeroots chloroform partition. The solvent system of different ratios of hexane, hexane/ethyl acetate, hexane/acetone, and acetone will use. Silica gel 60 (mesh 230-400 ASTM) will use to pack CC. In order to achieve good separation, mass ratio of silica gel to compound 20:1and 50:1 will use. 3.10.2Column Packing Before starting to pack a column, a small piece of cotton is gently will insert into the centre hole of column with the aid of a long stick.
Zeinab Ossaili - 7654795 Synthesis Lab – Experiment 1: Separation By Distillation The objective of this experiment is: • To use simple distillation to purify liquids. • To experience the limits of simple distillation when it comes to separations. • To use fractional distillation to separate mixtures of liquids. Method used: Distillation 1 – Distillation of an organic liquid containing a non-volatile coloured impurity • The distillation apparatus was assembled in regards to the instructions given and this was done by setting up the heating mantle followed by the round bottom flask, the reduction adapter, still head, thermometer adapter and finally the thermometer.
Then, it is air dried and followed by fixing it with flame from Bunsen burner. After fixing the smear, it must be stained using Gram staining solution, firstly crystal violet solution was flood onto it, and allowed for 1 minute, then wash off with tap water. Then, flood the slide with iodine solution for 1 minute and wash it off with tap water again. The formation of a dye-iodine complex will occur in the cytoplasm. Then, it was flooded with ethanol and washed immediately.
Step 2: Mix both test tubes , shake gently and time the reaction. Step 3: The same step as procedure 1, and step 3 which is to record the observed color step 4: use the palette/color chart to help you identify the observations you make. Safety precautions: Pull your hair back Safety eye goggles Closed toe
The plate is removed when the solvent front approaches the top of the plate and the position of the solvent front recorded before it is dried (this allows the Rf value to be calculated). TLC has applications in industry in determining the progress of a reaction by studying the components present; and in separating reaction
Water purification by using nanomembranes can provide solution to their problem. DISCUSSION Desalination processes that are mostly being used for water purification includes Reverse Osmosis, and filtration through nanomembranes. Both these processes use pressure driven membrane technology which is employed at various configurations. Reverse Osmosis Pressure is used to drive the water through the membrane, leaving the salt behind.
The water pipes If you immediately replace the bidet with another device, press and hold the lines in place, but leave them off until the new device in place. • Scrape off any mastic or sealant from the base of the bidet with the putty or utility knife • Loosen the screws that secure the bidet on the floor with the wrench. • Raise the bidet and away from his position, and move it. On the plastic film • Ask in the sewer, all gases can not escape the cloth or rag. What is a bidet
Purpose and Techniques: This experiment has the aim to determine a chemical formula of hydrated compound, which ingrains cupper, chloride and water molecules in its structure. In order to find this hydrated compound, it is necessary to use the law of multiple proportions. In other word, finding the appropriate variables values to this compound (CxCly*zH2O). Additionally, two major steps are required to proceed the experiment.
THE EFFICACY OF WITHANIA SOMNIFERA AGAINST Robert Glover Table of Contents Introduction 3 Method 4 Aseptic Technique 4 Sample Preparation 4 Extract Preparation 4 Disc Diffusion 5 Ethics 7 Results 8 References 9 Introduction Withania Somnifera is a plant with much potential, but is it most effective at inhibiting E-Coli and Staphylococcus aureus when in a solvent of Methanol or Ethyl acetate. This could assist in preventing and curing diseases caused by these bacteria, namely cholecystitis, bacteremia, cholangitis, urinary tract infection, and traveler 's diarrhea Method Aseptic Technique •
Purpose This experiment is to determine the concentration of the solute copper sulfate pentahydrate, and the unknown solution, by passing different wavelengths of light through each solution. Procedure Weigh out approximately 5g of copper sulfate pentahydrate. Record the mass and place the solute into a 50 mL volumetric flask. Fill half of the flask with distilled water, add the stopper for the flask, and lightly shake the flask, until the copper sulfate pentahydrate fully dissolved.
Introduction The purpose of this week’s lab was to enhance our understanding of the Grignard reagents that were examined in lecture. In this lab, a Grignard reagent will be prepared through the reaction of magnesium turnings and bromobenzene. Instead of isolating the product it will then be combined with benzophenone, which will give the final product of triphenylmethanol. Procedure