White Leg Shrimap Research Paper

CHAPTER 3
METHODS AND PROCEDURES
This chapter deals with the methodology used to identify the anticoagulant property of the sulfated glycosaminoglycan extract from the White Leg Shrimp (Penaeus vannamei, family Penaeidae).

Research Methodology
The research method used in this study is experimental. The sulfated glycosaminoglycan extract was obtained through extraction and isolation. Physical and chemical tests were conducted to identify the sulfated glycosaminoglycan. Instrumental tests were performed to identify and quantify the sulfated glycosaminoglycan. Biological tests were performed such PTT and PT, to identify the effectiveness of the sulfated glycosaminoglycan in the human blood plasma.

Research Procedure
The experiment was conducted

3.2 Solubility Test
The solubility test for the sulfated glycosaminoglycan extract from White Leg Shrimp was done using distilled water, petroleum ether, acetone, 95% ethyl alcohol, n-hexane and chloroform.
3.3 Chemical Test
3.3.1 Benedict’s Test
3.3.2 Fehling’s Test
3.3.3 Molisch’s Test
4. Instrumental Analysis for the Identification of the Sulfated Glycosaminoglyan Extract
4.1 FTIR
The sulfated glycosaminoglycan was subjected to FTIR at Centro Escolar University-Manila. FTIR was used for identification of organic molecular groups and compounds due to the range of functional groups, side chains and cross-links involved with use of Fourier Transform Infrared.

5. Biological Test
5.1 Determination of the anticoagulant test
5.1.1 Selection of Human Blood Donor
The blood samples were taken from five healthy women and five healthy men under the age ranging 18-25 years old, with a weight of 45kg-75kg who are not experiencing from any cardiovascular disease and dyslipidemic disorder. No history of using NSAIDs, not taking alcoholic beverages and smoking for atleast one week.
5.1.2 Collection of Human Blood