Staining Essays

  • Biochemical Test Lab Report

    1371 Words  | 6 Pages

    particular compound help them to be identified by the biochemical tests. Gram’s stain was originally devised by histologist Hans Christian Gram in 1884. Gram-positive bacteria stain purple, while Gram-negative bacteria stain pink when subjected to Gram staining. Approximately 60-90% of the Gram-positive bacterial cell wall is made up of peptidoglycan and interwoven teichoic acid, while only

  • Ink Lab Report

    1217 Words  | 5 Pages

    allowed to dry. Then, the smear must be heat fixed by exposing it to flame for few times until it got fixed. It is to prevent the cell from washing away during the staining and washing process. Then, it is air dried and followed by fixing it with flame from Bunsen burner. After fixing the smear, it must be stained using Gram staining solution, firstly crystal violet solution was flood onto it, and allowed for 1 minute, then wash off with tap water. Then, flood the slide with iodine solution for 1

  • Bacterial Colony Morphology

    1076 Words  | 5 Pages

    A single clone of bacteria multiplying in the presence of nutrients to form a visible growth on the solid medium is called as a bacterial colony. Colony morphology is one among the various characters of bacteria which is also unique to a particular genus of bacteria that could be instrumental in the preliminary identification. Size (measured in millimetres-pin point (≤1mm), small (2-3mm) , medium (4-5mm) and large (>5mm) colonies, shape (circular, irregular, rhomboid, umbonate, umbonate, filamentous

  • Gram Staining Lab Report

    818 Words  | 4 Pages

    University, Clemson, SC], personal communication). Gram used tissue in his first attempt with staining (1). The staining of the organisms allowed for the viewing of structure and determining those components by the viewing of the color that appeared after staining the organism (2). The purpose of this experiment was to identify the organism as gram-positive or gram-negative. The first step in the gram staining procedure was to heat fix the smear to the slide to ensure that the organism did not rinse

  • Endospore Staining Report

    998 Words  | 4 Pages

    Endospore stain is the fourth staining method used to add contrast. “Bacteria capable of producing endospores do not do so uniformly during their culture’s growth. Sporulation is done in response to nutrient depletion” (Leboffe). The theory to stain endospores cells is that keratin in the spore resist the stain so a more extreme measure must be taken to stain the bacteria. Malachite Green, the primary stain, is forced into the spore by steaming the bacterial emulsion (Leboffe). Malachite Green is

  • Azotobacter

    4892 Words  | 20 Pages

    REVIEW OF LITERATURE Beijerinck(1901) firstly reported, isolate and describe the species of Azotobacter such as A.chroococcum and A.beijerinckii. The former species was described as a soil inhabiting species and the later as a water borne organism. Sengupta, R.K. (1990) reported that the isolated Azotobacter species had ability to fix atmospheric nitrogen in pure culture.Mulder and brontonegoro (1974) described several other species of Azotobacter i.e. A.chroococcum and A.paspaii. Subramoney and

  • Enterococcus Faecium Lab Report

    845 Words  | 4 Pages

    Lab-experiment immunity and bacteria- How do they react? Research question: How does the bacteria Enterococcus Faecium SF 68 demonstrate resistance against the following antibiotics: Oxacillin, Climdacylin, Penicillin-G, Amikacin, Lincocymin, Erythromycin, Cephazolin, Mezlocillin ? Terminology used: Bacterium: Singular form of bacteria, one single individual. A bacterium an organism that possesses one single cell and is very adaptable to most environments. A bacterium contains only a single

  • Essay About Coconut

    1675 Words  | 7 Pages

    Chapter 1 INTRODUCTION Aspidiotus Rigidus or more commonly known as “cocolisap” is a destructive pest that attacks the Coconut tree by eating the leaves, fruits, and flowers until only the trunk is left (1). During infestation, the “cocolisap” completely covers completely the underside of leaves and defoliate, resulting to the shriveling of nuts leading to premature nut fall and even kill their host plants (2). The Philippines is the second largest producer of coconut products and, in some areas

  • Lab Report

    1402 Words  | 6 Pages

    3.0 MATERIAL AND METHOD 3.1 General techniques 3.1.1 Collection of sample Five sample was collected from Seri Manjung Mangrove (TSMa, TSMb, TSMc, ASMd, ASMe) and two samples from Batu Maung Mangrove area (BMa and BMb). The sample was collected inside a sterile falcon tube and transported immediately to Biology Lab at USM Biology School. Isolation procedure was immediately set in motion. The remaining sample was stored inside a 4oC refrigerator for further uses. 3.1.2 Weighing Weighing machine

  • Who Invented The Microscope Essay

    1720 Words  | 7 Pages

    Definition of microscope; the microscope is an instrument which is used for magnifying objects that are too small to see with the naked eye; such as cells, bacterias and other microorganisms (particles). It gives an enlarged image of the object. Definition of lens; a piece of glass, or another transparent material, with curved sides. Used for bending light rays (concentrating or dispersing) which passes through. The phenomenon that causes the light to bend when crossing the lens is called refraction

  • Essay On Advantages And Disadvantages Of Antibiotics

    974 Words  | 4 Pages

    Every individual wishes to be healthy and disease free. Occasionally, the human health gets negatively affected due to pathogenic, disease causing microorganisms. In such cases, one takes antibiotics to cure themselves from this condition. Another mechanism to deal with this problem is vaccination which a prevention mechanism. Let us take a look at both these methods to fight diseases. Antibiotics are chemical substances produced by some organisms, and can kill or inhibit the growth of other microorganisms

  • Biogas Production Experiment

    708 Words  | 3 Pages

    5.2.1. Results and discussions of 2nd experiment. (Co-digestion of water Hyacinth with cow-dung). The generation of the biogas from the batch reactor is measured daily with the help of a water displacement method. The biogas production for a period of 32 days was calculated. The initiation of the biogas production started after 48 hrs from the start of experiment. During first few days the gas productions was low and remain linear for a few days .The gas production was highest at the 18th days

  • Nitrogen Fixing Bacteria Lab Report

    1448 Words  | 6 Pages

    Effect of different physical conditions on nitrogen fixing bacteria from rhizosphere Hypothesis: Rhizospheric Nitrogen fixing bacteria show optimal growth at PH: 6-7, Temperature: 30 °C and Salinity level: 0.005 – 0.010M NaCl INTRODUCTION: There is a huge bacterial diversity in rhizospheric soil. Gram-negative, non-sporulating baccilli which respond to root exudates are predominant in the rhizosphere (Pseudomonas, Agrobacterium). While Gram-positive

  • Microflora Lab Report

    1368 Words  | 6 Pages

    was hypothesized that the nostril microflora were gram-positive bacteria that belong to the genera Streptococcus, Staphylococcus and Propionibacteria. Results showed that the nostril microfloras were gram-positive (stained purple). However, gram staining was not enough to prove that the bacterium obtained from the nostril are Streptococcus, Staphylococcus, and Propionibacteria spp. The results do not fully support the hypothesis. Introduction All Fastidious microorganism rely on specific nutrients

  • Salmonella Case Study

    2152 Words  | 9 Pages

    around Chennai, India. We studied the drug resistance of different microbes from clinical isolates based on the phenotypic character. The morphological characteristics of bacteria were observed through culture characteristics by carrying out gram staining techniques while the biochemical characteristics of bacteria were carried out by biochemical test. A total of 2423 samples were collected from suspected patients visiting different hospitals, primary health care center, and tertiary care hospitals

  • Antibacterial Soap Essay

    748 Words  | 3 Pages

    Abstract What type of bacteria or fungi or found on hands. There are ways to find out what lives on your hand one is taking a swab form your hand and put it on a petri dish allow thing to grew. This lab we found mainly yeast as for the fungi petri dish and for bacteria there was Staphylococcus epidermidis on the hand. So, after doing all of this we learned that there are many things living on your hand and that most of them are harmful. The thing that we found are commonly found in every hand and

  • Peromonas Hydrophila Lab Report

    747 Words  | 3 Pages

    24 hours. The obtained pure cultures were identified using the phenospecies and genospecies methods. The phenospecies characterization of A. hydrophila was performed in accordance with the Indonesian National Standard (SNI 7303, 2009), using Gram staining, motility test, oxidase test, blood agar test, oxidative-fermentative test and Rimler-Shoots+novobiocin test. 2.2 The isolation of

  • Essay On Sneeze Effect

    706 Words  | 3 Pages

    Have you heard about the "sneeze effect" that happened everytime you flush the toilet? “Sneeze effect” is a term originated by Dr Charles Gerba after he had conducted an experiment on the spread of bacteria when a toilet is flushed. The studies showed that each time the toilet is flushed, an invisible cloud of dirty water and fecal bacteria are projected out of the toilet bowl spreading up to 8 feet away. This spread of bacteria can survive up to 24 hours and transmit to the entire washroom in

  • Simple Staining Lab Report

    831 Words  | 4 Pages

    E.Coli and Bacillus sp. are used as sample in simple staining. Methylene blue is used in simple stain as a blue colour dye which makes bacterial cell blue colour. This enable cells become more visible under microscope. In simple stain, both gram negative and gram positive bacterial cells are blue colour as

  • Macbeth Blood And Staining Analysis

    579 Words  | 3 Pages

    The Tragedy of Macbeth, by William Shakespeare, continuously uses the Blood and Staining motif. The use of this motif emphasizes Macbeths deplorable need to be safely thus, as a tyrant would when murdering those who have cared for him. It also emphasizes character, corruption and death. Macbeths need to be safely thus as a king is a psychological reaction from having murdering a king himself and knowing that rulers aren’t completely untouchable. It also can be a reaction from having known he had