Staining Essays

  • Ink Lab Report

    1217 Words  | 5 Pages

    allowed to dry. Then, the smear must be heat fixed by exposing it to flame for few times until it got fixed. It is to prevent the cell from washing away during the staining and washing process. Then, it is air dried and followed by fixing it with flame from Bunsen burner. After fixing the smear, it must be stained using Gram staining solution, firstly crystal violet solution was flood onto it, and allowed for 1 minute, then wash off with tap water. Then, flood the slide with iodine solution for 1

  • Unknown Bacteria Lab Report

    1200 Words  | 5 Pages

    The purpose of this lab report is to employ a myriad of skills, tools and, methods learned throughout this semester to perform the appropriate tests for the identification of the assigned unknown bacteria. Add more background information here!!! The most important tools and techniques used during this identification include aseptic technique, microscopic examination and, the use of selective and differential media. Aseptic technique is an important tool for microbiologists. It is imperative that

  • Gram Staining Lab Report

    818 Words  | 4 Pages

    University, Clemson, SC], personal communication). Gram used tissue in his first attempt with staining (1). The staining of the organisms allowed for the viewing of structure and determining those components by the viewing of the color that appeared after staining the organism (2). The purpose of this experiment was to identify the organism as gram-positive or gram-negative. The first step in the gram staining procedure was to heat fix the smear to the slide to ensure that the organism did not rinse

  • Macbeth Blood And Staining Analysis

    579 Words  | 3 Pages

    The Tragedy of Macbeth, by William Shakespeare, continuously uses the Blood and Staining motif. The use of this motif emphasizes Macbeths deplorable need to be safely thus, as a tyrant would when murdering those who have cared for him. It also emphasizes character, corruption and death. Macbeths need to be safely thus as a king is a psychological reaction from having murdering a king himself and knowing that rulers aren’t completely untouchable. It also can be a reaction from having known he had

  • Enterococcus Faecium Lab Report

    845 Words  | 4 Pages

    Lab-experiment immunity and bacteria- How do they react? Research question: How does the bacteria Enterococcus Faecium SF 68 demonstrate resistance against the following antibiotics: Oxacillin, Climdacylin, Penicillin-G, Amikacin, Lincocymin, Erythromycin, Cephazolin, Mezlocillin ? Terminology used: Bacterium: Singular form of bacteria, one single individual. A bacterium an organism that possesses one single cell and is very adaptable to most environments. A bacterium contains only a single

  • Who Invented The Microscope Essay

    1720 Words  | 7 Pages

    Definition of microscope; the microscope is an instrument which is used for magnifying objects that are too small to see with the naked eye; such as cells, bacterias and other microorganisms (particles). It gives an enlarged image of the object. Definition of lens; a piece of glass, or another transparent material, with curved sides. Used for bending light rays (concentrating or dispersing) which passes through. The phenomenon that causes the light to bend when crossing the lens is called refraction

  • Examples Of Unknown Lab Report

    3385 Words  | 14 Pages

    Unknown Lab Report Abiola Oyewumi March 16, 2015 Unknown #16 Abstract An experiment was conducted to determine which of the following unknown bacteria was in test tube number 16: Escherichia coli, Enterobacter aerogenes, Klebsiella pneumoniae, and Salmonella typhimurium. Biochemical tests were used to help identify the unknown bacteria. The Citrate test, Urease test, Triple Sugar Iron Agar test, Voges-Proskauer test, and Methyl Red test were the biochemical tests used in this experiment. The T-Streak

  • Pelargonium Graveolen Observation

    662 Words  | 3 Pages

    Observations After conducting an experiment to find which plant, Tagetes erecta or Pelargonium graveolens inhibit bacterial growth. Results have shown that one plant, Pelargonium graveolens, prevented the growth of the bacteria, Staphylococcus, more efficiently and effectively than the Tagetes erecta. These results are evident as seen in the statistics provided above. The area around the Pelargonium graveolens sample just after day one was at 8 millimetres; day two increased to 9 millimetres and

  • Nitrogen Fixing Bacteria Lab Report

    1448 Words  | 6 Pages

    Effect of different physical conditions on nitrogen fixing bacteria from rhizosphere Hypothesis: Rhizospheric Nitrogen fixing bacteria show optimal growth at PH: 6-7, Temperature: 30 °C and Salinity level: 0.005 – 0.010M NaCl INTRODUCTION: There is a huge bacterial diversity in rhizospheric soil. Gram-negative, non-sporulating baccilli which respond to root exudates are predominant in the rhizosphere (Pseudomonas, Agrobacterium). While Gram-positive

  • Unknown Identification Lab Report

    568 Words  | 3 Pages

    The Unknown Identification Lab was an experiment that provided the opportunity to apply all the tests that were learned in the semester of lab, to identify the two bacterias that remain unknown. Gram- staining and two other tests will be used to identify the unknowns. This experiment is crucial to the understanding of each test, and can benefit in the ability to identify the characteristics of specific bacteria. Having a clearer understanding of the bacteria can further the research of bacteria for

  • Microflora Lab Report

    1368 Words  | 6 Pages

    was hypothesized that the nostril microflora were gram-positive bacteria that belong to the genera Streptococcus, Staphylococcus and Propionibacteria. Results showed that the nostril microfloras were gram-positive (stained purple). However, gram staining was not enough to prove that the bacterium obtained from the nostril are Streptococcus, Staphylococcus, and Propionibacteria spp. The results do not fully support the hypothesis. Introduction All Fastidious microorganism rely on specific nutrients

  • Biochemical Test Lab Report

    1371 Words  | 6 Pages

    particular compound help them to be identified by the biochemical tests. Gram’s stain was originally devised by histologist Hans Christian Gram in 1884. Gram-positive bacteria stain purple, while Gram-negative bacteria stain pink when subjected to Gram staining. Approximately 60-90% of the Gram-positive bacterial cell wall is made up of peptidoglycan and interwoven teichoic acid, while only

  • Essay On Advantages And Disadvantages Of Antibiotics

    974 Words  | 4 Pages

    Every individual wishes to be healthy and disease free. Occasionally, the human health gets negatively affected due to pathogenic, disease causing microorganisms. In such cases, one takes antibiotics to cure themselves from this condition. Another mechanism to deal with this problem is vaccination which a prevention mechanism. Let us take a look at both these methods to fight diseases. Antibiotics are chemical substances produced by some organisms, and can kill or inhibit the growth of other microorganisms

  • Latent Bloodstains In Crime Scene Photos

    1196 Words  | 5 Pages

    An initial review of the crime scene photos indicates the possibility of latent bloodstains being present in certain areas. Looking closely at the snapshots suggests that certain areas have been cleaned. The door is clearly dirty in certain areas, yet a large portion of it is inexplicably clean and very white compared to the immediate surrounding areas of the door. On the floor, one can see something very similar with the metal plates. All of them are dirty and dusty to some extent – except the one

  • Blood Agar Lab Report

    1057 Words  | 5 Pages

    the organism appeared purple with cocci in clusters. The organism was also catalase positive which means that it produced enzyme catalase and bubbled when hydrogen peroxide was added to it. Three test were conducted based on the result of the gram staining procedure. Blood agar with a Novobiocin disk was chosen as well as DNase (DNA) and Mannitol Salts (MSA) agar. The Blood agar is a bright red, opaque plate and the streaking or the inoculation technique was a modified streaking for isolation with

  • Unknown # 10 Essay

    650 Words  | 3 Pages

    Unknown #10 produced no identifiable macroscopic characteristics as a broth, so the first step was to Gram stain a loopful to determine the microscopic characteristics. Gram staining not only helped identify Unknown #10’s microscopic morphology but it also helped ensure the specimen was a pure culture—no other bacteria were visible when Unknown #10 was Gram stained and observed under the microscope. Unknown #10’s key microscopic morphology was that it was a very small, Gram negative bacillus. Though

  • Microbe 3c Lab Report Procedure

    389 Words  | 2 Pages

    Analysis of Major Unknown 3C Summary: A series of tests was done with Microbe 3C. A Gram stain was done and the sample was gram positive. A streak plate was completed revealing that the microbe was clear/white in color, margin was entire, shape was circular, elevation was convex/raised, and the opacity was translucent. The last test done was the sucrose test. It starts orange and turns yellow positive for microbe 3C Klebsiella oxytoca (Professor Brady, Personal Communication). Procedure

  • Unknown Bacteria Lab Report

    1034 Words  | 5 Pages

    Bacteria can be classified as gram positive or negative (difference in call wall). Gram positive bacteria have a thick cell wall of peptidoglycan (“polymer of disaccharides cross-linked by shorts chains of amino acids”), and stains purple after the staining procedure under a microscope (Todar, Kenneth,

  • Mystery Microbiology Test

    906 Words  | 4 Pages

    surrounded by cell enveloped called capsule, also some bacteria make capsule when faced in a harsh environment to protect them. A capsule stain was preform, the results were analyzed and observed. An additional procedure that was done, was the Fast Actin staining which helps to see if the bacteria contains Mycolic acid in their cell walls, which determines the structure and function of the cytoskeleton in living and fixed cells (Shah). As expected for both E.coli and K. Pnenumia the fast acting results were

  • Unknown Microbial # 398

    532 Words  | 3 Pages

    Methods Unknown microbial #398 went through several of tests in order to identify its characteristics when isolated from a urine sample of Doris, a 64- year old patient with a kidney infection. To identify unknown #398, must prepare a working and a reserve stock by the inoculation from a broth culture and by quadrant streaking method on a PEM and EMP plates. The following test procedures were incubated at 37°C for 48 hours for observation and identification for unknown #398. The identification of