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Pectinase Agar

1087 Words5 Pages

1) Isolation of fungi
The fungus is supplied by the supervisor. In this study, fungi Aspergillus versicolor will be used as the microorganism. Aspergillus versicolor will be placed on the Potato Dextrose Agar (PDA) medium. The microorganism will be sub cultured once in a week which then they will be incubated at 30⁰C to allow the growth of the active mycelium. Finally, Aspergillus versicolor will be stored at 4⁰C in the refrigerator on agar slants.
2) Agro-waste treatment
In this study, the agro-waste will be used as the main components for the solid growth medium. They include of sago hampas, orange peels and also rice husk. The agro-waste will be collected from the industrial factories, farm fields, local market or any other sources. First of all, the agro-waste will be washed and then dried them by oven-dried process at 70⁰C for 2 hours. Next, the substrate will be sieved and milled into smaller pieces. The substrate will be grounded by using an electronic …show more content…

1.1. Optimization studies for pectinase and cellulase production
The factors that involved in the pectinase and cellulase production investigated were types of mineral salts, urea concentration (mL) and amount of substrate (g).
i. Effect of different mineral salts :
The effect of different mineral salts on pectinase production was studied. The salts are calcium chloride (CaCl2), ferrous sulphate (FeSO4), K2HPO4 and KH2PO4. The concentration of mineral that will be used is 1.0 mL. Dissolve in the distilled water to adjust the level in the solid substrate. ii. Effect of varying urea concentration :
The effect of varying concentration of urea was determined. The ranges of urea concentration use are 0 mL (control), 0.5 mL, 1.0 mL, 1.5 mL, 2.0 mL and 2.5 mL. iii. Effect of different agro-wastes:
Agro-waste that will be used: Palm oil waste, sago palm waste, pawpaw peels, orange peels, wheat bran, pineapple peel, soybean

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