The membrane permeability of Beta vulgaris is affected by ethanol solution Introduction Cell membranes are the semi-permeable membrane that surrounds all cells. It separates the extracellular environment from the intercellular environment. It is a phospholipid bilayer which contains various proteins, lipids and carbohydrates all serving different purposes. It is this structure which allows for the transport of nutrients, proteins and water. (Nature.com, 2014). Through extensive testing it has been found that small alcohols, specifically ethanol can increase the fluidity and membrane permeability of the phospholipid bilayer (Patra et al, 2005). The aim of the experiment was to test what effect that ethanol solution would have on the membrane …show more content…
The aim of the experiment was to test what effects that ethanol solution has on the membrane permeability of B. Vulgaris. The B. Vulgaris samples were approximately 1cm3. They were kept the same size to ensure accurate results. A control test was conducted in distilled water to obtain a result to compare. The ethanol treatments were 40% and 70%. To prepare the solutions a 70% ethanol solution was used to make 40%. This was calculated using the C1V1=C2V2 formula. A photo spectrometer was used to measure, in arbitrary units, the change in membrane permeability of the B. Vulgaris cells. To begin, the B. Vulgaris samples were put into vials containing the distilled water, 40% and 70% Ethanol solutions. As soon as the B. Vulgaris samples were added to the vials a time zero sample was taken from the vials. 3mL of the liquid in each of the vials were added into cuvettes and measured in the spectrophotometer. Before each time point the photo spectrometer was zeroed using a cuvette with 3mL of distilled water. If any of the results were considered unusual the machine was zeroed again and the sample was retested. The results from the spectrophotometer test were recorded in a table. The experiment was repeated six times to gain a sample size of six. This was done to get more accurate results. The first time the experiment was conducted it was tested at three different time points, at zero minutes, fifteen minutes and …show more content…
More specifically the aim was to investigate what effect 40% and 70% ethanol solutions had on a B. Vulgaris cell membrane and then compare them to the same test with distilled water. It was hypothesised that the ethanol solution would increase the membrane permeability. From the results the hypothesis can be supported. The topic of cell membranes have been extensively researched, meaning that there is no limit to information and sources of information of the subject. The effects of alcohols on membrane have also been researched quite extensively. From this research and literature the expected results of the experiment were to see an increase in membrane permeability, this result was observed. The study provides a brief insight into what kind of effects alcohol can have on cell membranes but it is limited. The results can be used to understand but not explain more practical applications, like human cells for example. Conclusion The experiment found that ethanol increases the membrane permeability if B. Vulgaris cells. This can be extrapolated to conclude that ethanol will increase the membrane permeability of most cell walls. These results are concordant with most other studies into the topic of the effects of alcohol on membrane permeability. The results could possibly be used to investigate specific cases in laboratory and industry settings. For example,
Task 1: Write a letter to the editor Dear Dr Holzapfel: After reviewing the manuscript submitted by Pearson et al. , I suggest the paper to be revised before the acceptance for publication is granted. The paper hypothesised the predictive response of cell differentiation to be mathematically represented as a function of growth factors concentration in conjunction with shear stress level in a hollow fibre membrane bioreactor (HFMB).
Chemical stress affected the cell membrane of a beet cell, because of the higher amount of ethanol added to the beet. For example, we added 1% ethanol, 25% ethanol, and 50% ethanol to 3 test tubes with 15 mm of beets inside. We left it with no air inside for 30 minutes then tested the absorbance of the ethanol without the beet. The class got roughly 0.273 for the 1% ethanol, 1.205 for the 25% ethanol, and 1.882 for the 50% ethanol concentration. In each solution, the ethanol was a bit redder than the last.
NIH Senior Health states that when people drink beer, wine and other kinds of drinks, the alcohol goes to the bloods team and then it spreads throughout the body. Then the alcohol moves through the metabolism. Metabolism is a one of the converting substances that our body usually uses. It affects the brain and other more when alcohol is break down into our body. On the other hand, when people drink alcohol, the concentration of alcohol in the blood goes to a peak and it decreases when metabolism makes the alcohol down.
• In alcoholic cirrhosis, the portal vein becomes scarred and blocked, causing an increase in hydrostatic pressure. Which leads to an increase in hydrostatic pressure in the capillaries. Alcoholic cirrhosis also causes the liver to under produce albumin. This lowers osmotic pressure in the vasculature, enhancing filtration out of the
Diffusion and Osmosis Lab Report By: Jettica Williams BIOL 1107 Lab September 21, 2016 Prepared for Mrs. Fulford Lab Course Page Break The cell membrane act as a roadblock for cells. The cell membrane has a very hectic job. It restricts the access to what comes in and what goes out. The bond the membrane shares with others is the idea of accountability.
The importance of the experiment is to determine the effects temperature has on beet cell membranes, and to understand why certain cells are best suited for certain environments or in this case certain temperatures. Cell membranes play a vital role in selectively allowing substances such as proteins, nutrients, and other chemicals into and out of the cell. The cell membrane is also a line of defense against harmful chemicals and other agents that try to enter the cell. We hypothesize that increasing the temperature will increase the fluidity of the membrane thus making it less permeable. When the temperature of the cell increases it will cause the phospholipids that make up the plasma membrane to move apart from one another and become more
This article also discusses how alcohol can impair the recovery process of an athlete by causing a disruption of the white blood cells When reading this article, I consequently noticed that based off of different consumption of alcohol and the person, it will be very hard to determine the effect of alcohol in a general fact. Alcohol has many immune and endocrine system effects that impair blood flow and other system, causing strength, power, muscle endurance, etc. to
Part A: Osmosis practical task Aim: To observe the effects of osmosis in rhubarb cells. Hypothesis: Water will be extracted out of the cells in the salt solution causing the cells to look different to the cells in the freshwater solution. Materials: Rhubarb Distilled water in a dropping bottle Salt solution in a dropping bottle Microscope, slides, and coverslips Forceps and razor blades or scalpel Paper Towel Method: Clean and dry a slide and coverslip.
Title: How Ph Levels Affected the Fermentation of Beer Hypothesis: The beer will be left with more sugar deposit as the Ph levels increase because alpha/beta -amylase will no longer function. Predictions: Alcohol Percentage Analysis for the Control and the Experimental During this experiment, the pH level was increased, therefore Alpha-Amylase was favored. Due to the nature of Alpha-Amylase cutting randomly through a large carbohydrate molecule, it leaves bigger sugars in the flask, which cannot be digested by yeast. Due to this, less reactions should occur in the experimental, therefore leading to a lower percentage of alcohol production, compared to the control.
A Demonstration of Chemotaxis Between Flies and Various Substances (Sugar vs Bacteria) Abstract: The purpose of this lab based on the Drosophila melanogaster (the common fruit fly) reactions. Since the fly has been studied and observed for many years, and known for its unique chemotactic attractions to different stimuli, it was an ideal organism for the study being conducted. In the experiment the purpose was to be able to figure out whether the flies would be more attracted to sugar or bacteria.
Methanol, has a molecule containing CH3OH it being the smallest, ethanol having more carbons and hydrogens than Methanol comes second, CH3CH2OH, and propanol, CH3CH2CH2OH, is the largest of the three molecules. Aim: The aim of this experiment is to see the effects of different alcohols and concentrations on the biological membrane of the beetroot. Research Question: How do different alcohols and concentrations affect the biological membrane of a beetroot? Hypothesis: As the alcohols increase in toxicity and concentration, the damaging and breaking down of the membrane will increase leading to more red pigment being released into the solution causing the absorption of the solute to increase.
.4 Agar Disk Diffusion The antimicrobial tests were carried out according to disc diffusion tests (Lennette et al., 1985, Kim et al., 1995). Cells were streaked on MHA agar plate to obtain colonies at 37°C for 16 hrs after which they were resuspended in sterile saline solution to give an O.D of 0.1 at 600nm. On solidified 2% MHA plate, the bacterial culture is swabbed and sterile disc impression was made, 10 µl plant extracts was loaded on the agar plate.
Joshua Miller 12/18/17 Fermentation Lab report Introduction The term fermentation refers to the chemical breakdown of a substance by bacteria, yeasts, or other microorganisms, typically involving effervescence and the giving off of heat (wikipedia). Sugars are converted to ethyl alcohol when fermentation happens. In this experiment we determined if yeast cells undergo fermentation when placed in a closed flask with no oxygen. Glucose and yeast are mixed together in a closed flask and allowed to incubate for about one hour.
Chapter VII ANTIMICROBIAL PROPERTY Primary root canal infections are polymicrobial, typically dominated by obligatory anaerobic bacteria. The most commonly isolated microorganisms before root canal treatment include Gram-negative anaerobic rods, Gram-positive anaerobic cocci, Gram-positive anaerobic and facultative rods, Lactobacillus species and Gram-positive facultative Streptococcus species.83 The obligate anaerobes are rather easily eradicated during root canal treatment. In contrast, facultative bacteria such as non-mutans Streptococci, Enterococci, and Lactobacilli, once established, are more likely to stay alive even after chemo-mechanical instrumentation and root canal medication.84 In particular Enterococcus faecalis has gained attention