8- Repeat step( 6 ). 9- Wash the ester layer with 6 ml saturated sodium chloride , shake and allow two layers to separate . At this point ,The ester layer should not be cloudy . Again discard lower layer. 10- Transfer the ester layer to a small dry test tube and dry the ester with anhydrous CaCl2 and stir for 10 min.
Keep them in a colander; it will drain off extra brine and help Turkey to come to room temperature. This all process will take 30 minutes. 7. During these 30 minutes, prepare your oven by removing it racks and properly lining the bottom with aluminum foil. Also, pre-heat the oven for 165 degree
3. Use With Yogurt Ingredients Turmeric powder Yogurt How To Use Similarly, use turmeric along with yogurt to make a homemade anti-tan pack. For this, mix a little amount of turmeric powder with whipped yogurt. On face and hands apply the mixture on skin over the tan area. Wash the skin after thirty minutes.
These undesirable properties have been controlled by the use of EDTA for plate treatment . After the spraying, plates were left for at least 1hour at room temperature and then in an oven at 110oc for 1 hour in order to activate themit before use. Then, a 2µl of solutions of separate individual and mixture of reference standards (OTC and TC) in methanol were applied on HPTLC
Then the blower was turned on for sufficient duration and UV lights were switched on for one hour. LAF microbial contamination test (Settling plate method) A total of three petri dishes were prepared aseptically inside a laminar air flow (LAF), and then the petri dish was filled by pouring sterile Tryptone Soy Agar (TSA) liquid media and allowed to solidify. One test media was placed in the LAF cabinet, one test media is placed beside the LAF cabinet while the other the test media was placed near the door. The three petri dishes lid were opened and allowed to stand for 15 minutes and closed again. Then, the test media is then incubated at 37 ° C, for 18-24 hours.
Prepare a wet mount slide of the rhubarb tissue in distilled water only. View your slide under low power on your microscope, and then switch to high power. Draw a diagram of the field of view, and label. Irrigate your slide with the salt solution. Leave for a few minutes.
EC 3 are hydrolases, which forms two products from the substrate via hydrolysis. (Bach, et al. 1961) This is seen in the equation: L- Arginine + H2OL-Ornithine + Urea (Nelson and Cox 2008). The urea cycle is the procedure where ammonia is transformed into to urea. Throughout the urea cycle, the amino acid, arginine, is changes into ornithine- this is another amino acid when hydrated, that is when water was added.
Add deionized water to the volumetric flask to the 250ml mark on the volumetric flask. 13. Read the volume from the bottom of the meniscus. 14. Swirl the solution to ensure that the oxalic acid crystals are properly dissolved in the deionised water.
Making tea solution: Take around 3 gram of tea from tea bags and record the weight with an uncertainty of ± 0.001 using an high accuracy balance. Take 400 ml beaker add 200 ml of distilled water to it. Start heating it up with the bunsen burner until 150ml remain. (recommended ratio of 1 g of tea : 50ml of water) Measure the temperature of water and wait till it reach the expected value Add the tea leaf once the water is 150ml(some will evaporate during the heating) and keep the temperature at a constant degrees by using a water bath. Using a stopwatch to determine the amount of heating time.
 described the synthesis of isoxazolines via cyclisation of substituted chalcone intermediates in the presence of hydroxylamine hydrochloride and screened them for antimicrobial activity. Tejaskumar et al.  proposed the synthesis of isoxazolines derivatives and screened them for antimicrobial activity. Das, P. et. al  described the synthesis of spiro – isoxazoline and investigated the compound as anti – cancer agents.
After receiving an unknown mixture, the sample was streaked for isolation onto TSA, blood agar, and MacConkey plates. Each plate serves as a first step to identify the unknowns. The TSA (tryptic soy agar) can be used to do a gram stain, which differentiates gram-negatives from gram-positives, based on the structural make up of the cell wall (Carson, 2015). The blood agar plate is used to test for hemolytic activity, which is useful for distinguishing gram-positives. A MacConkey plate is selective by inhibiting the growth of gram-positives and differential due to the fermentation of lactose by certain gram-negative species.