The different methods for checking the physical, chemical as well as biological parameter of given water sample are explained as below:
1. Temperature : Temperature is checked by the thermometer.
2. pH : We can check pH of given water sample through pH testing machine.
3. Dissolved oxygen : The term Dissolved oxygen is used to describe the amount of oxygen dissolved in a unit volume of water. It measures the ability of water to support the aquatic organisms. Its procedure has following steps :
I. Take a water sample about 300ml in a conical flask.
II. Add 2ml magnous sulphate well below the surface of water by means of pipette followed by 2ml sodium azide solution in the same manner.
III. Mix well and precipitations start appear. Leave for
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Add 2ml starch solution and blue colour appear in the solution.
VI. Titrate it against EDTA (in the burette) until blue colour first disappear.
VII. Note the ml of EDTA used.
4. Biochemical oxygen demand: It is a chemical method to determine the amount of dissolved oxygen the aerobic organisms used in the water sample to disintegrate or break the organic substances present in the given water sample at a certain temperature over a five-day period. Its procedure is same as that to determine the dissolved oxygen but the reading are taken after five days.
5. Hardness : The purpose of this experiment is to determine the total hardness of water sample which is the sum of calcium and magnesium ion concentration, both expressed as calcium carbonate in mg/L. Following steps are followed to proceed the experiment:
I. Add 200ml water sample in a conical flask.
II. Add 2ml ammonium buffer followed by few drops of EBT indicator.
III. Titrate with EDTA(in the burette) until the colour changes from wine red to blue.
IV. Note the ml of EDTA solution
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Lift the comparator towards upward so that a plenty of day lght can enter into the cells and also to water present in the cell.
V. If the dissolved DPD tablet give a pink colour to the water, this ensures the presence of chlorine in the water sample.
VI. Compare the colour on the colour scale and match it to the nearest colour and then read the amount of chlorine present in the water sample from the scale.
VII. After the completion of test, remove the cap of the comparator and discard the water into the soil.
8. Biological test : This test is performed to check the presence of living organisms in the water body such as algae, bacteria, plankton etc. This is done with the help of microscope.
EXPECTED OUTCOMES
This research project expects to have the following outcomes by the end of the project:-
1. Measurement of the different contaminants in the fresh water.
2. Analysis of mechanisms required for the analysis of physical, chemical as well as biological contaminants.
3. To analyse that whether the water is able to drink and to be used for other purposes.
4. Analysis of causes and consequences of existing contaminants in the water so that we can remove them for its utilization.
5. Publication of report of the research
Question 4: List the 3 errors; • Adding too many drops of NaOH at the same time would affect the results because we can’t determine the exact equivalent point when the color changed. The results won’t be accurate and that will affect all the data that are dependent on the amount of NaOH to titrate. • Other error could be the hardness to notice a color change; we always use a white paper under the flask to determine when the color changes right away. And if we don’t use the white paper it will be hard to determine the color change and the amount of NaOH that was used to titrate it. • Also other source of error could be by not rising the burette with NaOH before we fill up with it, or it maybe they were rinsing it with a lot of NaOH which could affect the data recording for NaOH amount of titration.
Next, about 10 mL of both solutions, Red 40 and Blue 1, were added to a small beaker. The concentration of the stock solution were recorded, 52.1 ppm for Red 40 and 16.6 ppm for Blue 1. Then, using the volumetric pipette, 5 mL of each solution was transferred into a 10 mL volumetric flask, labelled either R1 or B1. Deionized water was added into the flask using a pipette until the solution level reached a line which indicated 10 mL. A cap for the flask was inserted and the flask was invented a few times to completely mix the solution. Then, the volumetric pipette was rinsed with fresh deionized water and
That mixture was then filtered through a coffee filter. Nine test tubes were prepared in order to perform this dye coupled reaction. One contained 5.0ml of the potato and pH buffer mixture, 2.0 ml of hydrogen peroxide, and 1.0 of guaiacol to serve as a blank for the spectrophotometer. Four test tubes were filled with 2.0 ml of hydrogen peroxide and 1.0 ml of guaiacol, used for measurement by the spectrophotometer, each. The last four were filled with 4.0 ml of the potato and pH buffer mixture and 1.0 ml of peroxidase.
Some of our results for the Dissolved Oxygen tests were 12, 11, 8, and 7. Those were the results of our teams Eutrophication
On October 8th, 2015 our class took a field trip to the Conodoguinet Creek to test the water quality. We did 3 experiments to help us conclude the quality. During the first experiment we observed the different types of organisms in the creek. We also took tests for eutrophication in the water. Another experiment we performed were acidity tests.
Goals The primary goal of this experiment was to identify an unknown compound by running various tests to determine the qualitative solubility, conductivity, and pH value of the compound. Tests were also performed for the presence of specific cations and anions in the compound. The second goal was to discover the reactivity of the unknown compound by reacting it with different types of substances. The third goal of this project was to calculate the quantitative solubility of the unknown compound in water.
To do the temperature and dissolved oxygen tests, stick the probe in the water, and it will show numbers. One will be the dissolved oxygen in ppm (parts per million) and the other will be the temperature of the water. To do the pH test, stick the pH paper in the water and compare the color it turns to the scale. To test nitrates, put clear water in a container and dirty water in another, and put powder in them. Shake them and then compare the color they turn to the scale.
phosphates and nitrates ) - which is when fertilizers < such as those on farms > can seep through the soil or even run down the soil - called runoff - into the creek causing the water to have more nutrients than it should. ) Depending on how much pollution there is depends on how much algae there is ( the more eutrophication -there is the more nutrients in the water- results in how much algae there will be.) We were also testing on the pH scale ( which measures the acidity or alkalinity of water ) goes from 0 - 14 where 6 - 0 is higher acidity and 8 - 14 is higher alkalinity ( alkalinity is what neutralizes/destroys acid. ) 7 on the pH scale is neutral and that is the ideal water for the ecosystem. The pollution that we were testing for is where the acid came from to begin with.
3mL of the liquid in each of the vials were added into cuvettes and measured in the spectrophotometer. Before each time point the photo spectrometer was zeroed using a cuvette with 3mL of distilled water. If any of the results were considered unusual the machine was zeroed again and the sample was retested. The results from the spectrophotometer test were recorded in a table. The experiment was repeated six times to gain a sample size of six.
If there is a color change, then it is known that protein is present in the solution. Finally, lipids are tested. 5 mL of water are added to 5 mL of oil. 5 drops of Sudan 3 are added, and if the color changes, then lipids are present. Next, the McMush is tested.
Glacial acetic acid and acetic anhydride were added to the mixture while refluxing, which converted the lime colored solution into a clear mixture. The flask was cooled in an ice bath and the solution
Some include, chemical testing, biological tests, and physical observations. Chemical testing consists of testing for the amount of nitrates, phosphates, the pH values, temperature, dissolved oxygen, and turbidity. These aspects of the river were all tested because they can show how healthy the river is. Testing these aspects is very simple but requires a tool kit. After the testing is done, in order to determine how healthy the river actually is, tests can be done to show the levels of chemicals, water clarity, the temperature and more.
And the last test I conducted was the dissolved oxygen. The average amount of dissolved oxygen is nine and eight tenths ppm. A certain amount of oxygen is dissolved in bodies of water. The more velocity in the water, the more dissolved oxygen can be found in it. The higher the amount of dissolved oxygen in the body of water, the healthier it is.
Next, I dye the Unknown with Gram’s iodine to create a complex only have on gram positive. The slide is rinsed by water after 30 seconds. Decolorization is the next step of the whole process. I let the alcohol flow on 45-degree angle slide within 15 seconds and wash it with water to remove colors on the surface. Lastly, the unknown is once again dyed with safranin for 1 minute then wash it off with water for the last time and dry it using bibulous paper.
In this experiment, the amount of water lost in the 0.99 gram sample of hydrated salt was 0.35 grams, meaning that 35.4% of the salt’s mass was water. The unknown salt’s percent water is closest to that of Copper (II) Sulfate Pentahydrate, or CuSO4 ⋅ 5H2O. The percent error from the accepted percent water in CuSO4 ⋅ 5H2O is 1.67%, since the calculated value came out to be 0.6 less than the accepted value of 36.0%.This lab may have had some issues or sources of error, including the possibility of insufficient heating, meaning that some water may not have evaporated, that the scale was uncalibrated, or that the evaporating dish was still hot while being measured. This would have resulted in convection currents pushing up on the plate and making it seem lighter by lifting it up