Sodium dodecyl sulfate polyacrylamide gel electrophoresis also known as SDS-PAGE is one of the methods for determining the molecular weight of unknown proteins. SDS is an anionic molecule which denaturizes proteins and brings it back to its’ primary structure and it also provides a negative charge to the uncharged molecule. The SDS-PAGE enables the separation of proteins based on their sizes. The larger the size of the protein, the harder it is to travel through the gel thus heavier proteins stay near the cathode side of the gel. For this experiment, a software named Gel Analyzer was used in order to obtain the molecular weight of the unknown proteins with the help of a protein ladder with known molecular weight and protein concentration.
Prokaryotic organisms normally have a cytoplasmic membrane, cell wall, and sometimes a capsule. Bacterial cells are most commonly either coccus or bacillus in shape. The cell wall is either Gram positive or Gram negative. When the cell is Gram negative, the cell has an extra layer of lipopolysaccharides. The Gram positive has a thick layer of peptidoglycan.
There are three types of endocytosis. One form is receptor-mediated endocytosis, which is where receptor proteins inserted in the membrane identify certain surface characteristics of substances to be included into the cell. Phagocytosis, or "cellular eating," is where particles larger than macromolecules are ingested. Pinocytosis, or cellular drinking," involves the capture of fluids. Pinocytosis is nonspecific and does collect particular
In order for an enzyme to carry out these functions it must work in conjunction with molecules such as substrates that are specific for each type of protein, and Pilar Feldbush General Cell Biology February 12, 2015 Lab Section K Lab 5: Enzymes coenzymes which aid in transporting the substrate to the protein’s “active site” (a hole or groove designed to fit only a specific type of substrate). Once attached, the protein can now move on to its destination, whether it be to the bloodstream, digestive system, or any other organ within the body. The ability for the substrate to attach to the enzyme is what allows the enzyme to hold and maintain it’s shape, which in turn directly impacts it’s function. The shape of an enzyme can be altered through the process of denaturation (the unraveling of the protein). Denaturing occurs when an enzyme is exposed to higher temperatures of heat and causing it to break the weak bonds that hold the molecule together.
In many cases the association process is a part of biological function as in blood clotting or the formation of muscle fibers. Aggregation of proteins also leads to perturbation of the biological function with sometimes serious physiological consequences as in the formation of cataracts in the lens of the eye or amyloid fibrils associated with Alzheimer’ and other neurological diseases. From a colloid chemistry perspective, protein self-association is a special case of the general problem of colloid stability. There are two important aspects of the protein systems in this respect: first in contrast to colloids in general the system can be obtained in pure form and then represent a true single component. Second the protein has a complex molecular structure and one should expect protein- protein interactions to be highly directional.
Biosynthesis Pathway A biosynthesis pathway describes the steps that take place in a chemical reaction which occurs when living organisms create new molecule from simpler ones. The word "biosynthesis" comes from two words: "bio," which means that the reaction is occurring in living organism and "synthesis," which indicates that large products are made up by simpler molecules. To describe a pathway completely some compounds are involved which includes such as which enzymes, coenzymes and cofactors are used in each reaction. Not all molecules are synthesized by humans, some molecules such as some essential amino acids i.e. lysine, these nutrients come from the protein rich food we consume including beans. Cells generally synthesize only those
These corneocytes under pressure lose their nucleus and become dead cells. Due to keratin, a fibrous protein the cytoplasm is usually flattened in nature. These cells also contain phospholipids and glycogen as their components. Stratum Lucidum Stratum lucidum consists of flattened epithelial cells. Most of the cells have degenerated nucleus and in some the nucleus is completely absent.
Proteins are complex macromolecules that are formed by elements carbon, hydrogen, oxygen and nitrogen. Proteins composed of one or more polypeptide chains of amino acids. The main functions of proteins are to structure, support, protect, make movement, catalyst, transport and make hormones in human body. In the structural role, collagen and elastin provide support for connective tissue. Actin and myosin are proteins that involved in muscle contraction and movement.
Cell Biology BI309 Mini-Review 1 Title: Dynein Motor Proteins In order for eukaryotic cells to be motile they use motor proteins that are propelled by ATP. There are three classes of motor proteins; myosin, kinesin and dynein. Dynein is the motor protein to be discussed in detail for this review.
It may limit the application of the PS cyclization as a chemical ligation method for peptides with N-terminal aromatic residue and peptides with aldehyde residue at C-terminal(40). 7. Pictet-spengler reaction for protein chemical modification= proteins are having aldehyde and ketone groups in their structures. So proteins are taken as a substrate and the pictet-spengler reaction is performed for making modification in the chemical nature of the proteins. P. agarwal and co-workers work for protein chemical modification by conducting a pictet-spengler reaction between aldehydes and alkoxyamines.
Cellular world can be divided into two types, depending on the presence of nuclei inside cells. Eukaryotic cells have a well-defined nucleus surrounded by a nuclear envelope, whereas prokaryotic cells lack this compartment. All bacteria are prokaryotes. Bearze (2015) provided information about the Bacterial Cell Structure and Function. Vibrio cholerae is a gram negative, non-spore forming, curved rod that is oxidase positive.
Inducers bind to the repressors and they also regulate gene expression. In the process of identifying the three strains of E.coli, ONPG (ortho-nitrophenyl b-D galactoside) was used as an indicator. ONPG is a substrate that can detect B-galactosidase, and when it does, it turns yellow. Sarkosyl was also a detergent used in the lab to lyse open cells. In the lab we predicted that the E.coli wild type would be clear for distilled water and sucrose but yellow for lactose.
Wolxy s(Vulpes Lupus) are the offspring of a red fox and a gray wolf. Both parents are members of the kingdom Animalia which makes their offspring a member of the kingdom Animalia as well. The offspring follows the same classification of its parents which is domain: Eukarya, phylum: Chordata, class: Mammalia, order: Carnivora, and family: Canidae. This offspring, like its parents, is a vertebrate which means that it has an endoskeleton that is supported by skeletal muscle tissue. It also has loose connective tissue and epithelial tissue that connects and protects its vital organs.