Gardenia Uliginosa Essay

PHYTOCHEMICAL SCREENING AND ANTI BACTERIAL AND ANTIFUNGAL ACTIVITY OF STEM OF GARDENIA ULIGINOSA

G. Sandhyarani1*, K. Praveen Kumar2
Post doctoral fellow .ucpsc,Kakatiya university,Hanamkonda,
Corresponding Author: [e-mail]
Ph no.8897474898

ABSTRACT In the Indian system of medicine Gardenia uliginosa is a potent medicinal plant. Gardenia uliginosa were studied for their Anti bacterial activity againstCandida albicans in the present study the aquous ,ethnolic,chloroform and petroleum ether extract of the.It was observed that the ethnolic extract and petroleum ether extract showed significant activity whare as aquoes extractshowed very less activity did not showed any activity against the tested fungal strain.

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At 370c Petri plates were incubated for 72 hours.

Zone of inhibition
The plates were inspected to identify zone of inhibition after incubation. Using the formula the diameter of zone of inhibition of each compound and the diameter of disc of different concentration was recorded.Zone of inhibition – Diameter of sample / Standard / control - Diameter of disc [.Kokate CK2005, Mishra SH 1978].

Ingredients Quantity prescribed Quantity taken Dextrose 40g 10g Peptone 10g 2.5g Agar 20g 5 g Dist water 1000 ml 250

Table 2. Percentage of extract obtained S/No. Parameters Estimated Percentage (w/w) Color Nature 1 Aqueous Extract 3.75 Green Gummy 2 Ethanolic Extract Light 3.66 Green Powder Powder 3 Pet. Ether Extract 2.50 Dark green Powder 4 Chloroform Extract 1.56 Green Powder

Table 3. Preliminary phytochemical screening of Gardenia uliginosa

S.No Constituents Test Aqueous Ethanolic Chloroform

followed by ethanolic extract 3.66 % w/w, petroleum ether. The scanty availability of 2.50 % w/w and chloroform extract 1.56 % w/w. This results are mentioned in Table 2. to study the Extraction, preliminary plant were subjected the various extracts of the attempt to phytochemical screening which phytochemical investigation and Anti bacterial activity of reveal the presence of various pharmacological active plant. components. The different concentrations of extracts were tested for Anti bacterial activity. These are prepared as coarse powderwhitch is subjected to most effective concentration was found to be of extraction using different solvents. Extractive values of Ethanolic extract are maximum because it gave the maximum Zone of various extracts were determined and the values indicate inhibition as compared to other threeextract and is the presence of considerable amount of constituents which found to be optimum as compared to standard drug Fluconazole. Using four different concentrations (20, 40, 60 & 80 ug/ml The study on fungi (Candida) was studied, On the basis of findings, the effect produced by extract was comparable to that of fluconazole anti viral drug. Zone of inhibition of test drug is