Thin Layer Chromatography Lab Report
Utilizing the techniques of acid-base extraction and Thin Layer Chromatography we were able to extract the three active components of Excedrin and confirm the identity of each compound. The three active components of Excedrin- aspirin, acetaminophen, and caffeine- all have varying acidities and polarity which allowed for the successful usage of acid-base extraction and TLC respectively. Acid-base extraction was applied by adding our compound to a basic solvent in order to isolate the components by turning them into water-soluble ionic salts. Then, by adding a stronger acid, like HCl, our components were protonated and precipitated out of the aqueous solvent. This creates crystals with can be analyzed with TLC. We extracted the components using …show more content…
TLC works based on different molecules travelling different speeds up a TLC plate based on polarity. TLC can be used to identify molecules and check purity. Since the three active components in Excedrin have different polarities, TLC was useful in identifying our samples based on how fast they ran up the TLC plate. We applied TLC by co-spotting our samples with the stock-room version of what believed them to be to see if they travelled the same distance up the plate. This application of TLC was useful because it allowed us to conclude the active component of Excedrin in each of our crystals. We were able to conclude that the crystals obtained from the K2HPO4 were aspirin since they both yielded a Rf value of 0.711. We also concluded the KOH crystals were acetaminophen with a shared Rf value of 0.504. Finally, we used TLC to determine the ether crystals were caffeine, yielding a shared Rf value of 0.209. We utilized how the three active components had varying Rf values due to the polarity of the compounds, with the lower Rf values corresponding to more polar molecules, in order to confirm the identity of our
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Paper Chromatography Lab Report
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TLC Analysis Of Analgesic Drugs Lab Report
843 Words | 4 PagesThey all have a stationary phase and a mobile phase. The mobile phase flows through the stationary phase and carries the components of the mixture with it. Different components travel at different rates (Harry W, 1989). For the reference plate, it can determine the Rf values of each analgesic (acetaminophen, aspirin, caffeine, salicylamide and index the reference mixture). There are two visualization methods used in the experiment.
Hi Dad Lab Report
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Chlorine 36 Lab Report
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Acetylsalicylic Acid Synthesis Lab Report
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Unknown B Lab Report
679 Words | 3 PagesUnknown A is Excedrin because they both look like white powders and they were both soluble. When the universal indicator was added they both turned red, which indicted their pH level was 4.0. Then when we tested the pH with the pH strips they both showed the pH as being 3.0. After that, we added HCl or stomach acid and both drugs dissolved and were soluble. We tested the pH and it dropped to 1.0.
Unknown B Test Lab Report
500 Words | 2 PagesWe identified unknown A as acetaminophen. This is because unknown A had all the same tested properties as acetaminophen when we performed our tests. For unknown B we found it to be acetaminophen and caffeine. This is because like unknown A, unknown B properties were the same as acetaminophen, but unknown B was acetone soluble. Unknown B has similar properties to caffeine because it was acetone soluble and because caffeine was the only basic pH tested to change color during the FeCl3 test.
Paper Chromatography Lab Report
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Phenacetin Synthesis
424 Words | 2 PagesThe results were an indicative for expected IR spectrum of pure phenacetin. The IR spectrum showed peaks at (3281.67 cm-1) which indicate the presence of the N=H stretch, (3131.40 cm-1 and 3073.97 cm-1) representing the Sp2 C-H stretches, (2982.26 cm-1, 2927.60 cm-1 and 2885.53 cm-1) expressing the Sp3 C-H stretches, also (1656.82 cm-1) indicating the C =O amide stretch, and finally a wavelength at (1603.51 cm-1) representing the aromatic C =C stretch of the phenacetin molecule, respectively. Such IR spectrum results from phenacetin in comparison with an acetaminophen IR spectrum clearly showed the elimination of the hydroxide (OH) bond present at the acetaminophen molecular structure, which resulted in the IR spectrum not indicating the presence of a strong and broad (alcohol-phenol) band at about 3500 cm-1
Temporary Emulsions Lab Report
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Nucleophilic Substitution Reactions Lab
1039 Words | 5 PagesTLC was used to identify the actual unknown product as well as other products/reactants present in the filtered solution. The procedure was conducted by placing a TLC plate in a developing chamber that is filled with a small amount of solvent. The solvent cannot be too polar because it will cause spotted compounds on the TLC plate to rise up too fast, while a very non-polar solvent will not allow the spots to move. The polarity of the spots also determines how far it moves on the plate; non-polar spots are higher than polar ones. After spots on the TLC form, the Rf values are calculated and used to analyze the similarity of the compounds.
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