Hsc300 Unit 9 Lab Report

Through the usage of gel electrophoresis the correct allele for each sample was able to be determined. Lanes one through three in the gel,were the positive control lanes they contained the PCR cocktail and a known high-quality template for the PCR reaction. First lane contained the sample with the +/+ allele, which had two copies of the ALU repeat allele. The first lane had a band at about 941 base pairs. The second lane in the gel contained the -/- allele and had its band at about 641bp, lower than the +/+ allele in lane 1. Since the +/+ has extra stretches of DNA, it should have a larger mass which makes it progress more slowly through the gel compared to the -/- allele which has less base pairs. The third lane contained the +/- sample, and after running the gel the lane had two different bands. This sample is heterozygous as it contained both alleles and thus had one band at about 941 base pairs and another band at about 641 base pairs. Lane four contained the negative control which only contained the cocktail mixture with water. There was no band present within this lane at it did not have any DNA present. The fifth lane contained student one’s PV92 DNA

Describe the main difference between a compound light microscope and a dissecting scope. The main difference between a compound light microscope and a dissecting scope is that a light microscope has a higher resolution and a dissecting scope displays the image in a 3D form.

Tn 4351 was originally isolated from bacteroides fragilis [30] . The transposon was successfully introduced into Cytophaga succinicans, Flavobacterium meningosepticum, Flexibacter canadiansis, Flexibacter strain SFI and Sporocytophaga myxococcoides by conjugation [25]. Tn 4351carries two antibiotic resistance gene. One of the codes for resistance to erythromycin and clindamycin which is expressed in bactroides but not in E.Coli. The other gene codes for resistance in tetracycline and is expressed in aerobically grpwn E. coli, but not in anaerobically grpwn E. coli or in bacteroides. The transposon of Tn4351 was originally detected in E. coli which carried an unstable chimeric plasmid, pSS-2. The mobilization of pSS-2 from onestrain of E. coli

An endospore is a dormant of a bacterial cell. It is a non-reproductive structure that ensures survival of a bacterium through stressful environmental conditions. Unknown #76, using aseptic technique, was inoculated to a nutrient sporulation medium (NSM) plate. This concerns a selective medium that increases the initiation of endospore production. A spore-former would have green-pigmented endospore cells when looked at under the microscope. From the growth on the NSM, I smeared it aseptically to a wet slide. Slide was then left to be air-dried for about 10 minutes. It was important to heat fix the slide using a micro incinerator. The succeeding steps had to be taken with caution because the primary stain, malachite green, is toxic. Under the hood, the slide was covered with a properly cut size of paper towel. The slide was then stained and left to steam with malachite green. It was continuously followed up by applications of the stain so it may remain moist for 10 minutes. The slide was then rinsed and safranin was again used as a counterstain. Using oil immersion objective lens of the microscope, unknown #76 had only reddish-pink cells without any signs of spore formation. Thus the given unknown is a non-spore former. Bacillus subtilis was used for positive control and Escherichia coli for negative control for endospore

In lab 3, fundamentals of chromatography, the purpose was to examine how components of mixtures can be separated by taking advantage of different in physical properties. A huge process in this lab was paper chromatography, which was used to isolate food dyes that are found in different drink mixes. The different chromatograms of FD&C dyes were compared to identify which dyes are present in each of the mixes. Chromatograms where made for the known FD&C and for the three Kool-Aid samples. The retention factor for each dye was calculated. F or each of the Kool-Aid flavors, 2.0 g was weighed out from the packet and 5mL of water was mixed in with them each. mL of 0.1% NaCl solution was added to 100mL of bottled water. The six chromatography strips

A milk-based, litmus broth tube is incubated and observed after 48 hours. Observations include lactose fermentation without gas as well as with gas, the reduction of litmus, casein protein coagulation and casein and protein hydrolysis. These characteristics were all determined based on the color of the solution and the production of a curd, the curds density and the production of a gas. To determine the density of the curd, the tube was slightly turned to see rather or not it was mobile or concentrated towards the bottom.

Increasing extracellular K+ reduces the net diffusion of K+ out of the neuron through the K+ leak channels because it caused to decrease in the concentration gradient.

ROYGBV (visible light) detected by light sensitive cells in the eye called rods and cones. Trichromatic

Within the current investigation, the effects of the surface area of Calcium Carbonate (CaCO3) in combination with Hydrochloric acid (HCl) upon its rate of reaction. CaCO3, commonly referred to as limestone, is an organic substance and is, in a sense, the crystallised “carbonic salt” of the element, calcium2. In addition to being a salt, the pH level of Calcium Carbonate is 9.91, and it is therefore, a basic substance, due to the fact that it is comprised of a pH level higher than 7, which is neutral3.

Regardless how much time is left in the shift,I 'd start by going over to the client who is injured and assist him. I would ensure that the client has clear airways and can breath. I would call for 9-1-1 and staff assistance. While emergency services are on the way I 'd look to see if there are any hazards in the area for us or the client. It 's important to stay calm and quiet with the client while we wait for emergency services to arrive. At this point ensuring the patient is calm and safe verses asking details of the event (until emergency services arrives) is key. Otherwise the client may get upset or go into shock over the event again.

1. You work as a medical researcher and have recently completed a study that shows the benefits to children of taking a daily vitamin. You want to communicate the results to two different audiences: The parents of patients at the hospital where you did the study, and the doctors there. Give an example of one way your communication to the doctors would be different from your communication to the parents.

Ribonucleotide reductase, the rate-limiting enzyme for de novo DNA synthesis, is an excellent target for chemotherapy. Its increased activity in cancer cells is associated with malignant transformation and proliferation.

Ammonia in butanol was the appropriate solvent to use for the column chromatography of food dye. After testing for the appropriate solvent, the set- up for column chromatography was done (Figure 2.).

The term chromatography actually means colour writing, and signifies a technique by which the substance to be examined is placed in a vertical glass tube containing an adsorbent, the different segments of the substance traveling through the adsorbent at distinctive rates of velocity, according to their degree of attraction to it, and producing bands of colour at different levels of the adsorption column. The substances least absorbed emerge earliest; those more strongly absorbed emerge later. (Wixom et al., 2011)

A gas chromatograph (GC) can be utilized to analyze the contents of a sample quantitatively or in certain circumstances also qualitatively. In the case of preparative chromatography, a pure compound can be extracted from a mixture. The principle of gas chromatography can be explained as following: A micro syringe is used to inject a known volume of vaporous or liquid analyte into the head or entrance of a column whereby a stream of an inert gas acts a carrier (mobile phase). The column acts as a separator of individual or chemically similar components. A column is typically packed with a stationary non-volatile matter (stationary phase). The separation occurs due to different interactions of each component with the stationary phase.