Proximate analysis Proximate analyses of raw materials i.e. moisture content, protein (using macro-Kjeldahl procedure), fat (using Soxtec 2045- Foss Instrument, Hoganas, Sweden), fiber (using Fibertec-Foss Instrument, Hilleroed, Denmark) and ash were carried out using standard procedures of AOAC (2000). Carbohydrates were calculated by difference. All the measurements were replicated, so that all the data in this paper are expressed as the mean of duplicate analysis. The proximate analysis of the flour mixture was 9.70% moisture, 14.30 % protein, 4.15 % fat, 1.24 % fiber, 1.69 % ash and 68.90 % carbohydrates.
Ltd. 4 Melting point Sentwin India 5 NMR Bruker Advance II 400MHz 7 Heating Mantle Inco 6 Structure builder Chem draw Ultra 8.0 4.2 Experimental work: 4.2.1 General procedure for Chalcones: 2’-hydroxy acetophenone or 2’-hydroxy propiophenone (0.2ml) and substituted benzaldehydes (0.5 g) were mixed in the round bottom flask. After that 40% NaOH solution (4g NaOH in 10 ml of distilled water) and ethanol were added in round bottom flask. The reaction mixture was stirred upto 6-48 hours. Completion of reaction was monitored in TLC plate (n-Hexane: Ethyl acetate 9:1). The reaction mixture was poured into ice cold water acidify with 1% HCl and precipitates were collected, filtered and dried and recrystalized with ethanol.
Add 3ml AlCl3 (10%) 5 min later. After 6 min, 2 ml 1 M NaOH was added and the total volume was made up to 10ml with distilled water. The solution was mixed well again, and the absorbance was measured against a blank at 510 nm with a (UV-VISIBLE Parkin Elmer Lambda 23 with win lab N6.0software.). The flavonoid content was calculated with quercetin as standard16. Reducing Power
3.2 Analysis of physicochemical properties of rice flour 3.2.1 Amylose content Amylose content was measured according to procedure reported by Juliano (1971). Flour sample of 100±0.01 mg (db) was measured into a conical flask and 1.0 ml of 95 % ethanol and 9 ml of NaOH were added. After that, sample was gelatinized by heating on a water bath (Yamato, BS 69, Japan) for 10 minutes. Amount of 5 ml of diluted solution was put in a 100 ml volumetric flask. Then 1.0 ml of 1.0
In the experiment, UV-Vis Spectrophotometer is used to determine the reducing sugar content of commercial natural honey by 3,5-Disnitrosalysilic acid (DNS) method. By scanning the standard solution (0.2, 0.4, and 0.6mg/ml) from 200nm to 800nm, the spectra with different concentration of standard solution is obtained. From the data, it clearly showed that the value of maximum absorbance increase with the increase of concentration of standard solution where we can see that the maximum absorbance for standard solution at 0.2, 0.4, and 0.6mg/ml are 0.406, 0.667, and 1.030 respectively. Then, three wavelength are chosen which are 490nm (up slope), 500nm (apex), and 510nm (down slope) and the graph of absorbance against concentration of glucose anhydrase
In addition, carbohydrates and lignin content of the plant residues have been reported in Table 2. Glucan was the dominate carbohydrate of the residues followed by xylan. In the case of the untreated seed cake, the lignin content was as high as 36.1%. 3.2 Pretreatment 3.2.1 Eruca sativa oil pretreatment Figure 1A shows the effects of esterification temperature and time on the FFA content of the pretreated Eruca sativa oil when a methanol to oil mass ratio of 0.4:1 was applied in the presence of 1 wt% sulfuric acid. The results showed that the reaction rate and yield were significantly influenced (See Table S1, Supporting Information) by both the reaction temperature and
But in the post test(45 days) experimental group one (gooseberry juice with honey) haemoglobin level significantly different from the control group and experimental group two( guava juice with honey). Within the group the pre test, 21 days and 45 days were compared by one way repeated measures of ANOVA. The ‘F’ and ‘P’ values of control group were 5.861 and 0.003 respectively gooseberry juice with honey group were 21.775 and <0.001 respectively. This result showed significant correlation in post test scores in control, experimental one and experimental group two
Stock culture: About 5,000 adult flies were maintained in steel framed cages (76 66 76 cm) covered with wired net. The flies were supplied with protein based artificial diets viz., (i) baking yeast: sugar: water at 1:3:4 ratio, and (ii) casein: yeast extract: sugar at 1:1:2 ratio. Water was supplied in a conical flask socked with cotton ball. Temperature (°C) and relative humidity (RH) of the rearing room was maintained at 27 ± 2°C and 75 ± 5%, respectively by using air conditioner (Model No. Movincool Classic Plus 26, USA).
Positive test is indicated by the development of cherry red colour . Quality control : Positive - Enterococcus faecalis ATCC 29212 Negative - Streptococcus agalactiae SPECIATION OF ENTEROCOCCI SUGAR FERMENTATION TESTS: For identification of species, 1% of sugars(Glucose, arabinose,raffinose,mannitol,sorbitol,sucrose,lactose ) in peptone water with Bromothymol blue (0.002%) as the indicator was used. To each tube of Sugars,1-2 drops of 18-24 hour BHI broth culture is added and incubated at 37 C overnight. Interpretation: Sugar Fermentation is indicated by the change of colour to yellow. Arginine Dihydrolase test : Moeller's decarboxylase basal broth with 1% Arginine along with an aminoacid free control were inoculated with the test strain.Both the tubes were overlaid with sterile liquid paraffin and incubated at 37 C overnight.The colour of the indicator reverting back to original (purple) indicating arginine hydrolysis was considered as positive provided the control tube remains yellow indicating fermentation.
6. Sue Bee Honey Sue Bee Honey Offerings Strength and Weaknesses Product - Clover Honey 237 grams @Php213.25 -Clover Honey Orange Blossom 237 grams @Php213.25 Strength: -Well-known brand -flavored variety of honey product -300 bee keepers members, known product even in US Weakness: -crystallizes in refrigerator, doubtful raw and pure honey Price - Clover Honey 237 grams @Php213.25 -Clover Honey Orange Blossom 237 grams @Php213.25 Strength: A little difference and low than the standard pricing Place -US based, -distributed in SM Supermarkets and Robinsons Strength: -US quality controlled, many market distribute in the country and internationally Weakness: -Doesn’t offer specific orders of delivery, just for market or physical store Promotion -Website, online, word of mouth Strength: -Website, online, Word of mouth. Already an established brand Weakness: -import brand cannot promote Filipino values Sue Bee Honey has a long and gratified tradition of supporting its American beekeepers. Countless of these processes have been passed down from generation to generation. ”Sioux Honey Association promotes its anti-slavery and anti-human trafficking policies by requiring that its direct suppliers pledge that the supplier is complying with anti-slavery and anti-human trafficking laws that exist in the country(s) in which a particular supplier is doing business”.