Analysis Of Tablet

ASSAY:
Preparation and analysis of Tablet:
For analysis of Cefuroxime Axetil and Linezolid in tablet, twenty tablets (Oratil LZ) which contain 500 mg of Cefuroxime Axetil and 600 mg of Linezolid was taken .Powder weight equivalent to 10 mg of drug was dissolved in a 100 ml volumetric flask and made up to half mark with ACN. It was sonicated till drug dissolve and was made up to mark with methanol (Stock solution). The Stock solution contains 100 μg/ml Cefuroxime Axetil of and 120 μg/ml of Linezolid.
From above stock solution pipette out 0.75 ml and transferred in to a 10 ml volumetric flask and the volume was adjusted up to the mark with Mobile phase to make final concentration of Cefuroxime Axetil 7.5 μg/ml and Linezolid 9 μg/ml.

ANALYTICAL METHOD VALIDATION:
The developed method was validated with respect to linearity, accuracy, precision,

Typically these might include impurities, degradants, matrix, etc.

 LINEARITY & RANGE:
 The linearity of Cefuroxime Axetil and Linezolid was found to be in the range of 2.5-12.5 μg/ml and 3-15 μg/ml respectively. Plot the calibration curve of area vs concentration (μg/ml). Linearity of both the drugs was checked in term of slope, intercept and correlation coefficient.
Preparation of calibration curve Aliquots of stock solution of Cefuroxime Axetil (100 µg/ml) 0.25, 0.5, 0.75, 1.0 and 1.25 ml and Linezolid (100 µg/ml) 0.3, 0.6, 0.9, 1.2 and 1.5 ml were pipette out in same five different 10 ml volumetric flasks and further diluted with mobile phase to obtain the concentration of about 2, 4, 6, 8 and 10 μg/ml for Cefuroxime Axetil and 4, 8, 12, 16, and 20 for Linezolid. 20 µl of each solution were injected into HPLC system and analyzed. Calibration curve was obtained by plotting respective peak area Vs Concentration in µg/ml and regression equation was obtained.

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