Croton Synthesis
Abstract The present investigation was carried out to analyze the active constituents present in aerial parts (dry seeds) of Croton bonplandianum Baill (Euphorbiaceae) for by GC-MS analysis. The presence of phytochemical compounds was screened by qualitative method. Totally 4 bioactive Phytochemical compounds were identified in the n-hexane extract of Croton species, the components were identified by comparing their relation indices and mass spectra Fragmentation patterns with those stored on the MS-Computer library and also form the published literatures. The prevailing compounds were Squalene, (9Z,12Z)-octadeca-9,12-dienoic acid, methyl 12-oxo-octadec-9-enoate -, and 11,14,17-octadecatrienoate. The research reveals the potential of hexane …show more content…
bonplandianum is having wide range of phytochemical compounds. Plant and leaves contain alkaloids sparsiflorine, crotoflorine, crotsparine, crotsparinine, proaporphine, isoquinoline dienone, N-methylcrotsparine and N-methylcrotsparinine. Leaves and stem contains ß-sitosterol and taraxerol, vomifoliol, ursolic acid and tetrahydroglazievine. Leaves also contain rutin. Seeds contain phorbol diesters, phorbol trimesters, cocarcinogen; alkaloid, 3-OMe-4, 6-di-OH-morphinandien-7-one and norsinoacutine [10]. The roots in addition to ß-sitosterol contain phenolic quinonoid alkaloid norsinoacutine and 3-methoxy-4,6-dihydroxy morphinan-dien-7-one. An unusual finding of this species is the hyper accumulation of copper in it [10]. The seed of Croton bonplandianum contains diterpines, phorbol ester, including 12-orthotrideconeoly-phorbol-13-acetat (TPA) and myristoyl phorbol acetate (MPA). TPA is a carcinogen, affecting prostaglandin …show more content…
collection (in the month of May 2015) from surrounding areas of rural areas of Vijayawada, Krishna District. The plant material was authenticated at the Botanical Survey of India, Howrah, West Bengal, India. Figure 1: Croton bonplandianum- Fruits, Blooms, Leaves Preparation of plant extract Preparation of plant extract The dry material of C. bonplandianum passed through sieve (70). The coarse powdered drug (200grams) was extracted in Soxhlet apparatus for 48 h with n-hexane (60-75°C, 2L). n-Hexane extract obtained was concentrated under reduced pressure in rotatory evaporator below 60°C temperature to get semisolid sticky residue (15gm) Column chromatography n-Hexane extract of the plant material (5 g) was subjected to column chromatography using silica gel (80-120 #) as adsorbent and eluted with the mixture of n-hexane: ethyl acetate in gradient manner. n-hexane: ethyl acetate (90: 10) fraction yielded dark brown color liquid. GC-MS Analysis GC-MS analysis of the extract was performed using a Perkin-Elmer GC Clarus 500 system and Gas chromatograph interfaced to a Mass spectrometer (GC-MS) equipped with a
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