The weak organic acid is largely un-ionized in the aqueous solution. No apparent change is observed when the reaction is carried out because the solutions of the starting materials and the products are colorless. The organic acid is soluble in water and thus, does not separate. It is necessary to extract the
3- Add 0.05 moles of concentrated sulphuric acid to round bottom flask very slowly and carefully then put a magnetic stirrer . 4- Set up reflux system using a clean and dry condenser .
In the round-bottom flask (100 mL), we placed p-aminobenzoic acid (1.2 g) and ethanol (12 mL). We swirled the mixture until the solid dissolved completely. We used Pasteur pipet to add concentrated sulfuric acid (1.0 mL) to the flask. We added boiling stone and assembled the reflux. Then, we did reflux for 75 minutes.
Then, the test media is then incubated at 37 ° C, for 18-24 hours. Rinsing reusable instruments The samples were rinsed with 40 ml of pyrogen-free water using a glass beaker that is free from pyrogens. Endotoxin testing using STV
25 mL of a 1 M phenyl magnesium bromide in tetrahydrofuran was dispensed into the beaker by using a syringe. The resulting mixture was stirred for about 15 minutes when the purple color turned into a brown color permanently. It was then extracted first with 20 mL of dichloromethane and the bottom DCM layer containing the product was reextracted with 10 mL of dichloromethane. The final bottom layer was retained and dried with MgSO4. The drying agent was discarded when the mixture was filtered.
To do this you first have to place the weighing boat on the scale and hit tare, so it reads zero and then place copper(II) sulfate pentahydrate on the weighing boat. Transfer the copper(II) sulfate into a 250-mL beaker. Rinse the weighing boat with 5 mL of purified water in small quantities to rinse off leftover chemical into the beaker. Spin the beaker gently to dissolve the solid. Rinse the sides of the beaker with small amounts of the 5 mL of purified water.
Finally, magnesium stearate (passed through a 60-mesh/250 micron screen) was introduced to the powder mixture. The final mixture was shaken manually for 5-10 min in a plastic bag. This powder was passed through the hopper of 16 station rotary tabletting machine and punched into tablets using 8mm s/c. the process is similar for all core formulations, which are prepared by direct compression technique. FORMULATION OF ENTERIC COATED TABLETS OF PANTOPRAZOLE SODIUM Formulation design: Pantoprazole enteric coated tablets were prepared using different polymers like HPMC 5cps (sub coat)
Weighed 1 gram of NaC2H3O2 and mixed it with ionized water. Boiled 12 mL of 1.0M Acetic Acid added into a beaker containing the sodium carbonate on a hot plate until all the liquid is evaporated
After adding three boiling chips, 10 mL of 48% hydrobromic acid was also added to the round bottom flask and swirled for 15 seconds to reactants in the flask. The reactants were clamped to a ring stand and a pre-set reflux apparatus with clear hoses attached to the condenser. The voltage regulator was set to 40 to begin water flow through the condenser and the application of heat, so the solvent can boil. The reaction was set to reflux for 30 minutes.
5. 150 ml of the solution in beaker A was added to the separating funnel with 10ml of chloroform. The funnel was gently shaken and vented to release the pressure. This was done five times. 6.
The chemiluminescence part of the experiment, we had to make four solutions labeled as ‘stock solution A, solution A, stock solution B, and solution B’. For the ‘stock solution A’ we put the luminol product, (0.242 g) in a 25 mL Erlenmeyer flask and dissolve it with 2 mL of 3M NaOH. Then we took 1 mL of the ‘stock solution A’ and diluted in 9 mL of water using a 50 mL beaker. Solution A. For the ‘stock solution B’ we mixed 4 mL of potassium ferricyanide solution and 4 mL of hydrogen peroxide solution using a 25 mL Erlenmeyer flask.
The purpose of this experiment was to learn about metal hydride reduction reactions. Therefore, the sodium borohydride reduction of the ketone, 9-fluorenone was performed to yield the secondary alcohol, 9-fluorenol. Reduction of an organic molecule usually corresponds to decreasing its oxygen content or increasing its hydrogen content. In order to achieve such a chemical change, sodium borohydride (NaBH4) is used as a reducing agent. There are other metal hydrides used in the reduction of carbonyl groups such as lithium aluminum hydride (LiAlH4).