The solution was stirred at room temperature for 8h. The solvent was blown out with nitrogen. The residue was added to 1 ml of water containing 0.1% TFA and purified on RP-HPLC. Massspec of the final product clearly indicates presence of RB modified on PEI by series of peaks matching different polymer compositions (see Fig. 6).
At pH 8 in presence of 5 ppm methylene blue dye solution just 3.0 mg adsorbent dose per 50 ml of dye solution significantly accelerate. This investigation will give a new insight in understanding the dye and heavy metal adsorption in natural environment. Keywords: Activated Carbon, Methylene Blue, SEM, Kinetics.
Figure 20 and 21 casting tray Figure 22 gel box After 1 hr., take out the gel from gel box carefully, place it into the machine, so that the DNA gel electrophoresis can be visualize under UV light. Figure 23 gel documentation system, used to visualize gel electrophoresis with UV light NMR spectroscopy First of all, 3 samples were prepared, peptide in SDS, DPC, and buffer. The sample temperature was maintained at 298 K, prepared by supervisor and H(hydrogen) in SDS and DPC micelle was replaced with D(deuterium) , so that in proton NMR, peptide won’t be interfered by H in micelle. amount of peptide in sds and dpc In the case of NMR of peptide alone, sample was prepared with 90% of H2O which is 540μL and 10% of D2O which is 60μL, so 600μL of solution was used to dilute 1.5mg of peptide. Put 600μL of sample into NMR Sample Tubes, put the sample tube into NMR sample holder, and then run the test, the chemical shift of proton in peptide can be monitored.
The total run time was 25.0 min. Regression equations revealed good linear relationships for Pt and Pz (correlation coefficients: 0.9986 and 0.9989 respectively). This assay offers advantages in terms of practicality and suitability for the analysis of phloretin and phloridzin with acceptable validation results such as linearity, sensitivity, and recovery in terms of RSD (%).The method was linear in the concentration range of 50–500 ng/mL. The limit of quantification (LOQ) and limit of detection (LOD) was 68.74 ng and 20.62 ng for compound 1(PT) and 61.89 ng and 18.57 ng for compound
A total of 0.1 ml of supernatant was added to cuvette containing 1.9 ml of 50mM phosphate buffer (pH 7). The reaction was started by the addition of 1 ml freshly prepared 30mM H2O2. The rate of decomposition of H2O2 was measured spectrophotometrically at 240 nm. Catalase values were expressed as n moles H2O2 consumed/min/mg protein. Measurement of lipid peroxidation TBARS, a measure of lipid per oxidation, was measured as described by Ohkawa .
Data Collection and Processing Raw Data Table 1: Amount of Iodine Reacting (± 0.05cm3) in the titration FT 1 FT 2 FT 3 FT 4 FT 5 Trial 1 1.9 1.8 1.8 1.5 1.6 Trial 2 2.1 1.8 1.7 1.5 1.6 Trial 3 2.1 2.0 1.8 1.5 1.5 Trial 4 2.5 2.3 1.7 1.6 1.5 Trial 5 1.9 1.8 1.6 1.6 1.4 Average 2.1 1.94 1.72 1.54 1.52 Analysis The results of Table 1 are shown graphically in Figure 2 where the calculated and measured Iodine Solution are plotted with respect to the amount of days. The graph can be observed, showing that the measured value of the iodine is very close to the theoretical one. It can be seen that each time the Vitamin C is frozen, the iodine solution reacting with the Acid decreases. This means that the Ascorbic Acid is being destroyed every time it is frozen. Volumetric Analysis Calculating the moles of iodine reacting Since the potassium iodide was mixed with the iodine to create triiodide so that the starch indicator could form the complex, it does not have to be
1.25mL of acetic acid and ferric chloride mixture was added to 2.5mL of the pure extract. Occurrence of blue-green color indicates the presence of glycosides. Test for Saponins. 10mL of distilled water was added to 5 mL of the pure extract the mixture was shaken in a graduated cylinder for 15minutes. Occurrence of layer of foam or bubbles indicates presence of saponins.
SYNTHESIS AND CHARACTERIZATION OF Mg-Cu-Zn FERRITES NANOPARTICLE BY CO-PRECIPITATION METHOD OXALATE AS PRECURSOR M.SATYA VANI Lecturer in Physics D.N.R.College, Bhimavaram Abstract Ferrite nanoparticles of basic composition Mg0.40,Cu0.25,Zn0.50Fe2O4 were synthesized through co-precipitation wet synthetic method by using oxalate as precursor at 90°C then filtered and washed with distilled water. After drying, heat treatment was carried out for 3 hours at 600°C and the resulting compounds were characterized for structural properties using X-ray diffraction [XRD], scanning electron microscopy, transmission electron microscopy, and Fourier transform infrared spectroscopy [FT-IR]. The XRD result shows that all prepared samples crystallite size are in the range of 30 nm to 60 nm and lattice constant in the range of 8.20 to 8.40 nm. Fourier Transform Infra Red Spectroscopy results clearly indicate the Mg-Cu-Zn nano Ferrite are synthesized. Differential scanning calorimeter graphs shows the phase formation of all the samples.
ASSESSMENT OF CADMIUM AND LEAD CONTENTS IN TISSUES OF TILAPIA AND CAT FISH SPECIES SOLD IN NSUKKA, NIGERIA Abstract The levels of cadmium and lead in the tissues of dried Clarias gariepinus and Oreochromis niloticus fish sold in Nsukka were investigated using Atomic Absorption Spectrometer. The study revealed mean Cd levels of 0.0015 and 0.0024µgg-1 for bones, gills and flesh respectively with a grand mean level of 0.0018µgg-1 in dried Clarias gariepinus. The average Pb concentrations of 0.0384, 0.0752 and 0.0299 µgg-1 for bones, gills and flesh respectively of the same dried Clarias gariepinus were also obtained with a grand mean level of 0.0478µgg-1. This study further revealed mean Cd concentration of the tissues of dried Oreochromis niloticus