The Biological Temperature Of Catalase

Research question What is the effect of temperature Amylase activity? Word count-1453 Background research Enzymes are biological catalysts that speed up a chemical reactions. They do this by decreasing the activation energy(the energy needed to start the reaction) of a chemical reaction. The enzyme present in our saliva is called Amylase. Amylase increases the rate of reaction by decreasing the activation energy needed to hydrolyse the starch molecules.

The effect of pH on the speed of enzyme interaction with substrate chemicals Hypothesis: About pH: If the pH level is less than 5, then the speed of the enzyme reaction will be slower. About temperature: If the temperature stays the same, then the speed of the enzyme reaction will not be completely affected. Background information: The function of enzymes is to speed up the biochemical reaction by lowering the activation energy, they do this by colliding with the substrate.

Results Figure 1. Effect of temperature on the reaction rate between catalase and H2O2 Figure 1 shows that the optimum temperature for catalase to catalyze hydrogen peroxide is around room temperature (30℃) as it has a very fast reaction rate (5). The overall trend is that temperatures that differ from 30℃, will decrease the reaction rate. Discussion This experiment supported the hypothesis, since catalase was the most effective with hydrogen peroxide when it was in an environment with a temperature of 30℃.

Title: Enzymes Abstract: Enzymes can catalyze chemical reactions by speeding up the chemicals activation energy. Temperature and pH are just two of the factors that affects enzymes and their involvement with chemicals and the way they function. Throughout this experiment, we conducted a study on peroxidase, which is an enzyme. The following information consist of the recordings of when it was exposed to four different pH levels to come up with an optimum pH and IRV at the end. Introduction: Enzymes are proteins that are used in reactions in living organisms.

Abstract This experiment showed that temperature, concentration and pH all affect the rate of enzyme reaction differently. Enzymes are very important in organisms and therefore understanding how and why they work the way they do in specific conditions is crucial. The results showed that an increase in temperature would also increase the reaction rate, until a temperature that was too high, where the enzymes began to denature and therefore the rate of reaction was slowed down. As concentration was increased, the reaction rate continued to increase.

In an organism 's body, chemical reactions are constantly taking place. These essential reactions can make or break the well-being of the body, yet the brain behind these changes is often times not recognized. This little brain or “macromolecule” is called an enzyme. An enzyme is a type protein that is able to speed up over 5,000 different reaction types an organism (2). Through catalyzation, the process of speeding up chemical reactions, enzymes attach to a substrate/molecule and break it down so that it can be used throughout the organism.

Enzymes speed up chemical reactions enabling more products to be formed within a shorter span of time. Enzymes are fragile and easily disrupted by heat or other mild treatment. Studying the effect of temperature and substrate concentration on enzyme concentration allows better understanding of optimum conditions which enzymes can function. An example of an enzyme catalyzed reaction is enzymatic hydrolysis of an artificial substrate, o-Nitrophenylgalactoside (ONPG) used in place of lactose. Upon hydrolysis by B-galactosidase, a yellow colored compound o-Nitrophenol (ONP) is formed.

Another factor that can affect the chemical reaction is temperature. It can affect the enzymes by changing their speed and at a certain temperature denaturing them. When enzymes and substrates are moving quickly, they are more likely to collide and have a chemical reaction. The warmer the temperature the faster the chemical reaction is to a certain degree. The chemical reaction was the most effective at 40 because the enzymes and substrates were moving fast, but the temperature was not high enough to denature the enzyme.

purpose the propose of this experiment was too see if the chemical reaction of a enzyme can be made faster. Hypothesis I think that a warm environment would be best to make an enzyme’s reaction faster. because a protein can move faster in heat.

Observing the effects of a catalyst on an enzyme’s rate of reaction Leong, M., Kim, E., Nair, A. Achilly, K., 9/22/2015 Introduction: An enzyme is a protein that acts as a biological catalyst. A catalyst increases the rate of reaction by reducing the activation energy required (Reece 2005). Catalase, an enzyme produced by most living organisms, catalyzes the decomposition of H2O2 in our bodies in order to maintain homeostasis.

An enzyme is protein that acts as a catalyst. Catalyst is a chemical agent that increases a chemical’s reaction rate by decreasing the activation energy (initial energy). In this experiment we used Turnip Peroxidase as our enzyme. It was primarily designed to find out if changing different factors such as, the enzyme concentration, temperature, pH and an inhibitor could have an effect on the enzyme’s activity.

In this lab, the optimal environment for an enzyme was observed in bacterial and fungal amylase. An enzyme is a substance produced by a living organism that is coded by proteins to catalyze chemical reactions throughout the body. Enzymes are what make the chemical reactions in living organisms possible. Enzymes act as catalysts to reactions, they lower the activation energy needed for reactions. However, enzymes require particular environments to function, and without proper conditions, enzymes

Throughout this work, a correlation between the enzyme activity and temperature existed. The data for both showed that at the temperature extremities the lower the enzyme activity was; except for the bacterial enzyme at 0°C. At high temperatures, an enzyme denatures or changes shape, making it difficult or impossible for a substrate to bind, and at low temperatures, the frequency and rate of reaction decreases causing for a halt in product formation (Pitzer et.al., 2012). Thus, showing that enzymes need to be in their optimal environments to work properly. Bacillus lincheniformis and Aspergillus oryzae are both organisms that live that in medium temperature environments.

They can only quicken reactions that will eventually occur, but this enables the cell to have a productive metabolism, routing chemicals through metabolic pathways. Enzymes are very specific for the reactions they catalyze; they make sure the chemical processes go in the cell at any given time. Peroxidase was the enzyme being testing in this experiment. A peroxidase is an enzyme that acts as catalysts, which occurs in biological systems. Peroxidase is found in plants, which they play a role in helping to minimize damage caused by stress factors or insect pests.

The purpose of this study is to investigate the effects of varying the concentration of peroxidase on rate of reaction, as well as, the varying temperature and pH levels. Enzymes are proteins that catalyze biochemical reactions that work by reducing the activation energy for each reaction, causing an increase to the rate of the reaction. One class of enzymes are known as peroxidase. Peroxidase catalyze the oxidation of a particular substrate by hydrogen peroxide. Meaning that it eliminates H2O2 in order to prevent damage to the cells and tissues (Department of Biology University of Tampa 74).