Unknown Lab Report Mikee Lianne Gonzales Biol 351- 1005 Holly Martin Unknown: # 76 Abstract This report is about identifying the respective genus of the given unknown organism. The goal is to show and prove the student’s understanding of microbiology and laboratory learned experimental techniques. The student is to use studied tests to eliminate possible generas and to isolate the correct genus and identify it to the given unknown. This proves the diversity of microorganisms and their uniquely varying traits. The resulting data obtained in class will enhance students’ laboratory skills and knowledge regarding microbial laboratory. Introduction The purpose of this study is to identify the genus and species …show more content…
Selective medium involves medium with environmental conditions that specifically grows some microbes while inhibiting others. Differential medium is used to identify and differentiate (as the title says) closely related microbes based on growth responses and physical indicators. It is imperative to use laboratory positive and negative controls in identifying the unknown because it confirms and compares the results of the unknown’s response to the definite guide. While performing the procedures in this report, students had to keep the bacterial and biological species concepts in context. The bacterial species concept is the identification and naming of microbes based on relating physical and physiological features of the unknown to the fitting taxa. This concept created a guide on naming all Bacteria and Archaea. The biological species concept on the other hand, does not take physical appearance into account on comparing and contrasting organisms but defines a species as part of populations that potentially interbreeds in nature. Students cannot classify microbes within the guidelines of a …show more content…
An endospore is a dormant of a bacterial cell. It is a non-reproductive structure that ensures survival of a bacterium through stressful environmental conditions. Unknown #76, using aseptic technique, was inoculated to a nutrient sporulation medium (NSM) plate. This concerns a selective medium that increases the initiation of endospore production. A spore-former would have green-pigmented endospore cells when looked at under the microscope. From the growth on the NSM, I smeared it aseptically to a wet slide. Slide was then left to be air-dried for about 10 minutes. It was important to heat fix the slide using a micro incinerator. The succeeding steps had to be taken with caution because the primary stain, malachite green, is toxic. Under the hood, the slide was covered with a properly cut size of paper towel. The slide was then stained and left to steam with malachite green. It was continuously followed up by applications of the stain so it may remain moist for 10 minutes. The slide was then rinsed and safranin was again used as a counterstain. Using oil immersion objective lens of the microscope, unknown #76 had only reddish-pink cells without any signs of spore formation. Thus the given unknown is a non-spore former. Bacillus subtilis was used for positive control and Escherichia coli for negative control for endospore
Methods Unknown microbial #398 went through several of tests in order to identify its characteristics when isolated from a urine sample of Doris, a 64- year old patient with a kidney infection. To identify unknown #398, must prepare a working and a reserve stock by the inoculation from a broth culture and by quadrant streaking method on a PEM and EMP plates. The following test procedures were incubated at 37°C for 48 hours for observation and identification for unknown #398. The identification of unknown #398 followed test procedures from Brown1.
The unknown bacteria was then tested on multiple selective and differential media. Growth was present on the MacConkey Agar and the colonies were the same color as the plate, which told me my bacteria was gram negative and did not ferment lactose. There was no growth on the Mannitol Salt Agar, and this told me the unknown was not salt tolerant and did not
Aseptic technique was initiated at the beginning of this experiment by cleaning the work surface with disinfected wipes. Personal protectives equipment was also worn. The material utilized in this experiment was: S. epidermidis culture broth, sterile cotton swab, streak plate, forceps in 70% alcohol, a lit tea light, and the three antibiotic disks (novobiocin, gentamicin, penicillin). The first step, I divided a plate into three quadrants and labelled them with the different antibiotic names. Using the lit tea light, like a bursen burner, I flamed the mouth of the S. epidermidis culture.
Being able to identify unknown microbes from systematic testing is what makes the field of microbiology so important, especially in infectious disease control. Using the testing procedure laid out by the microbiology field we are able to identify unknown bacteria present in our everyday lives, and along the way learn a lot about their characteristics that separate them from other types of bacteria. Being able to do this is vital in order for us to understand why microbes are present in certain places, how they are able to grow and what restricts their growth, that way they can be combatted if necessary. These techniques for determining unknowns are also important for isolating and testing infectious disease microbes in order to prevent spreading. Another important aspect of being able to identify unknown microbes is the
Microbiology Unit 4 Exam Answer the questions fully, but do NOT more than is need to answer the questions (don’t write on and on and on….). Do Not COPY AND PASTE from any source. Please use your own words to answer. No plagiarism. You Must use Turnitin to submit the exam.
This piece of writing is likely to be inspired from that book. Taking everything into consideration, we can sum up that microorganisms are categorized in six subdivisions including Bacteria and viruses. It is obvious that each of them is characterized by variable features and functions. And it is our duty to expand our knowledge and awareness in order to prevent probable health problems. Reference 1.
Worksheet 6 (Part2) Microbiology In section 2 of worksheet 6 you will be asked to answer a series of short answer, critical thinking and case study questions. Please consider each question carefully and then answer in your own words. Below is the rubric that will be used to evaluate your answers.
The fungus grew rapidly with a colony diameter that ranged from 55-70 mm after 5 days of incubation at 28-30 ˚C. A. niger showed white color at the beginning and turned black with rough/ granular surface as the colony matures. The reverse side is pale yellow in color when the mold was still young and quickly turned brown with cleft/ fissures when it matured. The mycelia of the fungus were hyaline and septated. Conidiophores were also found to be hyaline and bore a globose vesicle which was covered entirely by phialides that radiated around the vesicle and appeared to be biserate. The conidia were globose with dark
Results: In the experiment we saw six locations show bacterial samples. These locations were the soap dispenser in the men’s bathroom, the inside front door handle to building H, the first floor elevator button, the first floor water fountain button, and both the men’s and women’s bathroom trash can rim. Between the six locations, there was a species richness of at least one in all but four locations.
The word ‘bacteria’ is conjugated with tiny living beings or in other words microorganisms. Bacteria are unicellular organisms or a prokaryotic organism meaning that they have no nucleus and consist of a single cell and come in all different shapes and sizes, the most common shapes are the bacillus, coccus and spirilla shapes. Bacteria don’t belong to the plant nor the animal group but belong to a group all by themselves. They are single-celled microorganisms and are only a few micrometres thick, and are found normally in together in clumps of millions. How does it grow?