Enterobacteriaceae Essays

KPC-producing organisms), then the class of antibiotics called carbapenems will not work to kill the bacteria and treat the infection. Klebsiella species are examples of Enterobacteriaceae, a normal part of the human gut bacteria that can become carbapenem-resistant. CRE, which stands for carbapenem-resistant Enterobacteriaceae, are a family of germs that are difficult to treat because they have high levels of resistance to antibiotics. Unfortunately, carbapenem antibiotics often are the last line

a negative test result because unknown bacteria 5 utilized the butanediol pathway instead of the mixed acid fermentation pathway. The negative result of the Methyl red test eliminated approximately half of the remaining genera in the family Enterobacteriaceae in the 9th Edition of “Bergey’s Manual of Determinative

Since it was determined that the unknown bacteria is gram negative, it is the member of the Enterobacteriaceae. The test is successful because the media turned blue, which indicated that the bacteria is citrate positive because they're able to utilize citrate as their sole carbon source. They also able to convert the ammonium phosphate to ammonia and

as a source of nitrogen for many bacteria. They can also act as a final electron acceptor. Many bacteria can be differentiated and are identified by their capacity to reduce nitrates to nitrites. Most of the bacteria belonging to the family Enterobacteriaceae reduce nitrates [165]. OF test is used to differentiate those organisms that utilize carbohydrates aerobically (Oxidation) such as P. aeruginosa, from those that utilize carbohydrates anaerobically (Fermentation) such as members of the Enterobacteriaaceae

The gram negative species are apart of the Enterobacteriaceae family. These are gram-negative rods, which are facultatively aerobic - either respiring or fermenting. Most are motile via a flagella (Carson, 2015). Salmonella enterica and Yersinia enterocolitica are both paracolons, which lack the ability to ferment lactose. Conversely, Klebsiella pneumoniae and Escherichia coli are both coliforms, which are able to ferment lactose. Of the Enterobacteriaceae family, there are genera that are in the normal

fermentation it involves inoculating of sucrose broth with inoculating loop. Usually done for the differentiation of Enterobacteriaceae species. A positive result gives yellow color after the addition of indicator. A negative result shows no change in color or has red color. In glucose fermentation inoculation of broth is done by using inoculating loop. This test distinguishes Enterobacteriaceae family. This test is done for testing bacteria ability to ferment glucose and gas production. Yellow color shows

The bacterium was determined to be Gram- negative rods. The first attempt of gram stain the unknown appeared to look positive and cocci. But, the next day onwards for 4 consecutive days the unknown showed up as a gram- negative gram stain and appeared pink rods. Triple Sugar Iron Agar is a differential medium. It differentiates Gram-negative enteric bacteria based on the ability to reduce sulfur and ferment carbohydrates (lactose, sucrose, and glucose). This is indicated by the formation of acid

One of the most necessary aspects of criminal justice is forensic science, which involves the study of scientifically inspecting physical evidence, which has been collected from a crime scene. Some people define forensic science as being the application of science to law enforcement. You can use forensic science in the most challenging situations, for example, if there are no witnesses to a crime, or any leads, it can usually mean that forensic evidence is all the court has to work with. For example

Contamination with pathogenic bacteria from the human/animal reservoir can occur when the landing place is unhygienic or when the fish are washed with contaminated water. Upon the processing of fish, its exposure to water is a critical part as pathogens may be present in the water used which can cause contamination and spoilage (Shoolongela & Schneider, 2012). Contamination of food can occur because of problems in food production, preparation, storage or cooking. The aquatic environment and soft

My partner and I drew number eight for the unknown experiment. Throughout the semester, we did many tests to find out what our bacterium was. We discovered that our bacterium was called Serratia marcescens. At the beginning of the semester we made a stock culture prep that we could continuously use to find out our bacterium. Also, in case anything happened to the stock culture prep we made a reserved slant. We did many tests throughout the semester to find out the cell shape, arrangement, size, motility

prevent microbial growth. Q2G: Lipopolysaccharide - Lipopolysaccharide is a complex lipid structure containing unusual sugars and fatty acids found in most gram-negative Bacteria and constituting the chemical structure of the outer membrane. Q2H: Enterobacteriaceae - Enterobacteriacaea is a family of gram-negative, anaerobic, rod-shaped bacteria that are usually motile and consist of saprophytes and parasites of worldwide distribution. They can be found in soil, water, plants and animals. Q2I: Mutation

Microbiologist. Klebsiella begin in soil and the water on plants. K. pneumonia is found in the digestive and respiratory systems causing lobar pneumonia, meningitis, urinary tract infections, and would infections. Klebsiella is part of the family Enterobacteriaceae and belongs to the genus Klebsiella. This bacterium is nonmotile, rod-shaped, and gram-negative bacilli. K. pneumonia is facultative anaerobic meaning they can grow with or without oxygen. They do not form spores and are capable of forming

Surgical site infections (SSIs) are frequent and serious complications of surgical procedures. They are associated with a prolonged duration of hospitalization, readmissions, re-interventions and the patient may suffer from permanent disability or even death.1 SSIs are probably the most preventable of the health care-associated infections. Despite the widespread international introduction of level I evidence-based guidelines for the prevention of SSIs, such as that of the National Institute for Clinical

Nature. 213 (5072): 137–9. Bibcode:1967Natur.213..137M. doi:10.1038/213137a0. PMID 4291593; Berg JM, Tymoczko JL, Stryer L. Chapter 18 Oxidative Phosphorylation. Biochemistry. 5th edition, New York: W H Freeman; 2002). Many bacteria such as Enterobacteriaceae and Staphylococcus are facultative anaerobes. Aerotolerant Aerobes This group of bacteria get their energy from fermentation alone but unlike anaerobes they are not affected by the presence of oxygen. An examples of such as bacteria is Lactobacillus

Introduction: Microbiology is the study of the microbial world, which is composed of microorganisms that are too tiny to be seen with a naked eye. This project demonstrates the significance of correctly identifying unknown organisms with methods and techniques acquired in the laboratory as they can make or break one's ability to accurately isolate and identify various species. Studying microbes enables oneself to have an insight on not just their structure or how they function, but how they have

perform method of isolating and enumerating Salmonella spp. in a food sample. 1.2 Hypothesis To achieve a negative results in food sample with the absence of Salmonella spp. 1.3 Introduction The genus Salmonella belong in the family of Enterobacteriaceae is a group of rod-shaped, Gram-negative, and facultative anaerobic bacteria, which grow with or without oxygen. It is also a non-spore forming and a motile or non-motile bacterium with exception of S.gallinarum and S.pullorum. The genus Salmonella

Introduction The purpose of this experiment was to apply the knowledge gained from this semester of microbiology. A single, unknown bacterium was provided on a nutrient agar plate. Several microbiological tests were performed using sterile techniques that were learned to determine the identity of the unknown. Tests Conducted The first step to identify the unknown was to isolate the bacteria that was provided. This was done using a streak method on a nutrient agar plate. After one week the bacteria

Serratia: Serratia’s a group of gram (-negative), facultative anaerobic, rod-shaped bacteria of the Enterobacteriaceae family. Serratia are most common and pathogenic of the species within the genus, S. marcescens, is often the sole infective agent and frequently causes health facility infections. S. marcescens is often found in showers, bathroom bowls, and around wetted tiles. The bacterium is associate timeserving, human infective agent, capitalizing on its ability to make integrated surface communities

Isolation of Bacteria For this part of the project, we were each provided with an unknown bacteria. In order to obtain pure cultures of the unknown bacteria we had to use the streak plate technique to isolate separate colonies. We used a nutrient and MacConkey agar plate to begin. We used both agars because the nutrient agar allows us to grow the largest number of different types of microbes, yet not all bacteria can grow because it is rich with beef broth and some yeast extracts. McConkey agar

A surgical site infection is an infection that occurs after surgery in the part of the body where the surgery took place. Surgical site infections can sometimes be superficial infections involving the skin only. Other surgical site infections are more serious and can involve tissues under the skin, organs, or implanted material. Surgical site infection (SSI) is defined as an infection occurring within 30 days after a surgical operation (or within 1 year if an implant is left in place after procedure)