Laboratory glassware Essays

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    Elodea Lab

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    Elodea” was put into a tank labelled “chemically treated Elodea”. Three drops of phenolphthalein were added to each beaker and then all of them were placed on a sheet of white paper. 0.02N NaOH was slowly added to the “tap water with Elodea” beaker with a dropper that delivers 0.025 mL per drop. Each drop was counted and the solution was stirred thoroughly before adding the next drop. This was continued till a faint pink color appeared in the solution. The number of drops was noted. The procedure

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    Materials and Methods: To gather the needed data on the crayfish, samples were collected at Pearson’s Creek, a little outside Springfield. The spring was right off the road and located directly next to a little over pass. Sampling occurred early morning 0830-0930 on a cloudy day. The weather was a typical mild late summer morning with a rain shower that had passed through the evening before. Two sampling areas that were 10-meters long and the width of the stream were marked off using flags

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    Introduction 1.1 Aim: To determine the kinetic parameters, Vmax and Km, of the alkaline phosphatase enzyme through the determination of the optimum pH and temperature. 1.2 Theory and Principles (General Background): Enzymes are highly specific protein catalysts that are utilised in chemical reactions in biological systems.1 Enzymes, being catalysts, decrease the activation energy required to convert substrates to products. They do this by attaching to the substrate to form an intermediate; the

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    After pumping for awhile, you will probably be able to stockpile a large amount of breastmilk in your freezer to use while you are at work or away from your baby for the evening. When it 's time to use your breastmilk, it 's important to thaw it correctly in order to keep it safe and healthy for your little one. How to Safely Freeze Your Breast Milk After you are finished pumping, immediately pour your breast milk into a plastic storage bag or bottle. Leave about one inch of room at the top of

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    Assay of ATP-sulfurylase activity For the determination of ATP-sulfurylase activity in plant 0.3g fresh samples (leaf and root) were ground with chilled mortar and pestle using ice cool buffer consisting of 10 mm Na2EDTA, 20 mm Tris–HCl (pH 8.0), 2 mm dithiothreitol and approximately 0.01 g ml-1 insoluble polyvinylpyrollidone, using 1 : 4 (w⁄ v) tissue to buffer ratio. The homogenate was strained through gauze and centrifuged at 20 000 g for 10 min at 4 °C. The supernatant was used for the in vitro

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    Alka Seltzer Lab Results

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    Experiment 1: Materials: • Alka-Seltzer tablets • Empty and clean water or soda bottles (12 oz to 24 oz) • Balloons • Water • Clock • Stove top Procedure: 1. Pour a sufficient amount of water (about 16 oz) into a small pot and place on the stove at high heat. 2. Watch the clock and after 30 seconds take the water off the heat. 3. Drop one Alka-Seltzer tablet into one empty water bottle. 4. Pour the hot water from the pot into the bottle. 5. Stretch the open end of the balloon around

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    Synthesis of nanoparticles 1mM aqueous, and ethanol solution of silver nitrate was prepared for synthesis for silver nanoparticles. 1ml of plant crude extracts was added upon the various concentrations of silver nitrate solution such as 1mM, 3mM and 5mM and abrupt color changes indicates silver synthesis. 4.8 Oxalic acid preparation: The 100 mg of oxalic acid was weighed and dissolved in 100 ml of double distilled water for chemical silver synthesis. 4.8.1 Oxalic acid silver synthesis: 1mM of ethanol

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    In this experiment, racemic 2-methylcyclohexanone was reduced using sodium borohydride as a nucleophile to give a diastereomeric mixture of cis and trans secondary alcohols. The products were analyzed for purity using IR spectroscopy and gas chromatography. 1.2 g of 2-methylcyclohexanone and 10 mL of methanol were combined in a flask and cooled in an ice bath. Two 100 mg portions of sodium borohydride were added to the flask and stirred. 5 mL of 3M sodium hydroxide, 5 mL of de-ionized water, and

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    Exercise 2 began with measuring the milliliters needed to fill a coffee mug and measuring the liters in a gallon. Then we went to the sink and filled a graduated cylinder with 70 ml of water and then placed a pencil in the water. Our lab partner, Temi had to use both of her hands to push the pencil in the water in order to completely submerge it, because it kept floating upward. After completing these steps, we then calculated and recorded the volume. Once we finished this, we then repeated steps

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    Lab 2 – Water Quality and Contamination Experiment 1: Effects of Groundwater Contamination Table 1: Water Observations (Smell, Color, Etc.) Beaker Observations 1 There was no smell 2 When I pour the oil in the water I noticed the oil started to bubble to the top. So I got to stir the oil that’s when there were smaller bubbles. As I smell the water there was no smell to the water. Putting the vinegar I didn’t smell anything but I did see the water was a change it was dark that’s when I added the

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    Isopropanol

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    SDS-Polyacrylamide gels were prepared and the glass plates were washed with 70% ethanol and water. After drying the plates, water was used for test leakages. Two SDS-Polyacrylamide gels were prepared according to the following recipe. These all above components of the running gel were added in a 50 ml tube and solutions were mixed and pipetted into the prepared gel chambers. Glass plates were filled ¾ and the gel was covered with 100-500 µl Isopropanol in order to achieve an even surface. When the

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    There are many techniques that may be utilized in order to prevent, detect, and deter shoplifting. Roberson and Birzer describe a few of these techniques in “Introduction to Private Security”. Considering shoplifting is a common problem among businesses, many companies utilize one or more of these techniques in order to deter and prevent loss. In today’s world, technology is becoming more and more advanced every day. In turn, prevention and detection techniques are becoming more advanced and effective

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    Microscopic Analysis

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    Characterization of Ethosomes Microscopic Examination of Particle Size and Shape Microscopic analysis was performed to determine the average size of ethosomes [7]. A sample of ethosomes was suitably diluted with distilled water in order to observe individual vesicle and a drop of diluted suspension was put on a glass slide covered with cover slip and examined under microscope (magnification 15 × 45 X ). All measurements were performed in triplicates and the formed vesicles were spherical in

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    First, the group was assigned an amount of copper sulfate. Then, one group member measured out the copper sulfate while another member retrieved a beaker; it was filled with water. Both items were taken to the lab counter where a beaker was already placed. The copper sulfate was poured into the beaker. Afterwards, the beaker was placed on the hot plate. A thermometer was also placed in at an angle. After ten minutes the thermometer was adjusted until it was completely straight in the middle of the

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    Gummy Coca Cola Have you ever been home alone and tired of having nothing to do? Well, then you should make a gummy Coca Cola. Gummy Coca Colas are gummies that are shaped like a Coca Cola bottle and taste like a crisp, sweet Coca Cola. In my experiences, gummies need to be precise, so gummies need to be measured precisely. In order to create this tasty treat, you will need 8 packs of gelatin, a 500 milliliter bottle of Coke, and 100 milliliters of hot water. If you follow these basic steps, you

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    Experimental Clay-catalyzed dehydration of cyclohexanol Cyclohexanol (10.0336 g, mmol) was added to a 50 mL round bottom flask containing five boiling chips, Montmorillonite K10 clay (1.0430 g) was then added to the cyclohexanol and the mixture was swirled together. The flask was then placed in a sand bath and attached to a simple distillation apparatus. The contents of the flask were then heated at approximately 150 °C to begin refluxing the cyclohexanol. The distillation flask was then loosely

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    Trigonometry: Procedure

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    Procedures 1.First thing needed is a plastic bag open the plastic bag and take about a teaspoon of calcium chloride, put the calcium chloride in one corner of the bag. Then take about a half teaspoon of sodium bicarbonate and put it in the opposite corner of the bag. Then lay the bag flat on the table use about 5 mL of phenol red. Once the phenol red is in quickly lift your bag and put all the substances in one corner. Then observe 2.Reference procedure one for first step.Lay bag down and add about

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    Procedure: Obtain an empty aluminum can, ice bath, metal tongs and a hot plate Turn on the hot plate to maximum temperature Add a very small amount of water to the empty can and place it upright on the hot plate Wait until you see steam rapidly escaping the can and then use the tongs to quickly turn it upside down in the ice bath Observations: -vapor came out the top and bubbles were visible inside (boiling) -as soon as the can was flipped into the ice water, it was crushed -audible sound as the

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    Carotenoid Lab Report

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    Title of Invention "AN IMPROVED PROCESS FOR THE PREPARATION OF CAROTENOIDS FROM ENCYSTED HAEMATOCOCCUS CELLS" Abstract An improved process for the preparation of carotenoids from Heamatococcus cells characterized in simple method of extraction of said caratonoids by using acidified aqueous solution, which comprises suspending encysted Heamatococcus cells in acidified aqueous solution having concentration in the range of 0.01 N to N at a temperature range of 8-90° C for a period of 30 seconds to 15

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    Experimental procedure (real life) Complete the ‘experimental setup’ for the guinea pig ilium experiment as shown in Fig 1. The agonist, acetylcholine is added to the organ bath at a concentration of 1 x 10⁻⁸ M where it will follow the 3 minute cycle. After 30 seconds the results are recorded. After results are recorded, washing of the organ bath and ilium is conducted (note. Called wash out). After 90 seconds a second washout occurs. At 180 seconds the length of the guinea pig ileum is reset to

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