Thin layer chromatography Essays

Introduction The purpose of this experiment was to identify the composition of over-the-counter analgesics by the method of Thin Layer Chromatography (TLC). The TLC method is used for rapid qualitative analysis of mixtures to determine and identify its components and purity. A development solvent was used to separate the analgesics found in both the known drug sample and an unknown sample of over-the-counter medications. The pertinent techniques for this experiment are spotting the stationary phase

Thin Layer Chromatography Lab Report Miranda Tyree Lab Partner: Caitlin Price TA: Yi Zhou Lab Time: Thursday, 12 pm Lab Room: 207 Date: 09/23/2016 Abstract Experiments were performed to determine the active chemical ingredients in Tylenol and Anacin. The active chemical ingredients were determined by performing thin layer chromatography of four substances: Tylenol, Anacin, acetaminophen, and acetylsalicylic acid. The identity of the ingredients was then confirmed by using the co-spotting thin

Utilizing the techniques of acid-base extraction and Thin Layer Chromatography we were able to extract the three active components of Excedrin and confirm the identity of each compound. The three active components of Excedrin- aspirin, acetaminophen, and caffeine- all have varying acidities and polarity which allowed for the successful usage of acid-base extraction and TLC respectively. Acid-base extraction was applied by adding our compound to a basic solvent in order to isolate the components by

Thin Layer Chromatography (TLC) Abstract This experiment uses the TLC chromatography technique to identify the presence of acetylsalicylic and Acetaminophen in analgesic drugs (Tylenol and Anacin). It was found that the Anacin and acetylsalicylic had very closer Rf values (0.8 and 0.79). The Tylenol and acetaminophen had closer Rf values (0.54 and 0.58). Hence, Acetylsalicylic acid and acetaminophen were present in Anacin and Tylenol tablets respectively. Introduction Chromatography is the technique

methods used for the analysis of phospholipids. All systems of chromatography consist of a stationary and mobile phase. A monster placed on a stationary phase, i.e., a solid or a liquid, and the mobile phase, a gas or a liquid, is allowed by modifying the system. The components of the sample will be separated on the basis of their ranging physical and chemical properties, imparting different affinities for the two phases. Thin layer chromatography (TLC) was the first chromatographic method for assessing

under ultraviolet light or spraying the plate with a chemical reagent. The distance a spot travels up a thin-layer plate can be assigned a numerical value known as the Rf value. Theory: The technique of thin-layer chromatography (TLC) uses a solid stationary phase and a moving liquid phase to separate the constituent of a mixture. A thin-layer plate is prepared by coating a glass plate with a thin film of a granular material, usually silica gel or aluminium oxide. This granular material serves as the

triethylamine are used. Thin layer chromatography will be run at a specified time in the reaction, and the results will be examined both quantitatively(via ImageJ) and qualitatively to which reaction reacted more quickly. With this information, the stereochemistry of the alcohol can be deduced. Theory: The competing enantioselective

cyanobacteria mainly use chlorophyll (green) and phycocyanin (blue), it also contains carotene (orange) and xanthophylls (yellow). We deducted an experiment to investigate the photosynthetic pigments of cyanobacteria using the electrophoresis and thin layer chromatography (TLC) methods to divide and examine the pigments. The electrophoresis

NMR analysis. For the Melting Point Range Determination, samples of our re-crystallised p-aminophenol and paracetamol were placed into capillaries and into the melting point apparatus where data was recorded during the melt. Then, for the Thin Layer Chromatography, a TLC plate was prepared with four small dots loaded with p-aminophenol, our recrystallised p-aminophenol, our resultant paracetamol and commercial Panadol® respectively. When TLC was completed, it was transferred to a UV lamp box for analysis

Gurleen Bhangoo Robinjot Kaur CHEM 3301-02 Professor Jeffery Crisman 21 March 2023 Abstract: Chromatography is an analytical method that is used to separate a mixture of chemical compounds into their individual components, allowing the individual components to be thoroughly analyzed. In this experiment, thin-layer chromatography (TLC) is performed to identify an unknown drug, and then column chromatography is used to separate out the components of the drug. The given unknown will contain one of the

Ritchmond P. Togonon, Abiather L. Tsai, Jordan Martin G. Uy Department of Sports Science, College of Rehabilitation Sciences *philliptuazon14@gmail.com Abstract This experiment is about the separation of pigments in capsicum annuum using column chromatography. In this experiment pigments in capsicum annuum or red pepper were separated. Four different eluates with different colors were produced from the column by adding eluents through the column. Keywords: capsicum annuum, eluate, eluent, dichloromethane

After the assigned reaction was complete, samples of authentic cis-cyclohexene-1,2-diol, authentic trans-cyclohexene-1,2-diol, a 50:50 mix of the cis and trans cyclohexene-1,2-diols, and the product were each spotted on the Thin Layer Chromatography (TLC) plate. Then the TLC plate was placed inside a saturated beaker filled ethyl acetate in order to develop the plate. Once the solvent traveled up the solvent front, the plate was stained with anisaldehyde solution and then heated with a heat gun so

TLC Analysis of Analgesic Drugs Introduction The purpose of this experiment was to use thin-layer chromatography (TLC) to determine the composition of various over-the-counter analgesics (acetaminophen, aspirin, caffeine and salicylamide). The methods necessary was thin-layer chromatography Experiment Scheme Prepared at least 12 capillary micropipets to spot the plates. Then obtain two (silica) TLC plates and handle them carefully or the adsorbent may flake off. Handled them only by the edges. Used

In thin layer chromatography, the example is separated in the flexible stage dissolvable. The ensuing course of action is then spotted near the base of the rectangular sheet of glass or plastic secured with silica. The sheet is then submerged in a chamber containing a little measure of dissolvable, keeping the spots over the surface of the dissolvable. Once the sheet is set in the chamber, the dissolvable begins to increase on the sheet through thin movement. The covering on the sheet is the staionary

The purpose of this experiment was to determine the identity of isolated separated compounds using thin layer chromatography and column chromatography methods. In part A, four TLC were ran once each with ethyl acetate, hexane, toluene, DCM as solvent to separate a mixture. The stock solution is a mixture of benzophenone, biphenyl, and diphenyl methanol. The last trial include the comparison with the standard using the best solvent. It was predicted that toluene and hexane were the best solvent

Chromatography of Spinach Formal Discussion This lab involved the extraction of pigments from spinach leaves which were then analyzed using thin layer chromatography. The first step of this process was to grind up the leaves in order to extract the pigments. Hexanes facilitated this process and afterwards, the solution was dried over sodium sulfate to remove water. Next, the column was packed with a small piece of glass wool, followed by hexanes, and then packing sand. At this point, the column

3.1 Phenolics in buckwheat honey Figure 1 illustrates the wide variety of HPLC profiles of six buckwheat honey samples. As can be seen, these samples could be divided into two main types. The first type exhibited very similar chromatograms, including samples 1, 2, 3 and 4. The retention time of most compounds is 20 to 70 min. Conversely, the second type include sample 5 and 6. Their chromatograms appeared simpler and showed only a few lower level peaks. The figure showed the highest peak is

Photosynthetic Pigments Lab The aim of this lab is to separate photosynthetic pigments by chromatography, a method used to separate the components of a mixture, and to measure each pigment’s Rf value. There are different types of chromatography, and the one which this lab focuses on is paper chromatography. The components of the mixture, in this case, spinach leaves mixed with acetone, are pipetted at one end of the chromatography paper and hung in a jar slightly above solvent vapor to create the reaction.

to learn about chromatography; the separation of a mixture through a medium (the chromatography paper) in which the components move at different rates and create different patterns. We chose to explore this topic by using four different colored markers, placing a dot of each color onto one piece of chromatography paper, and observing while the water in the beaker travels up the paper and separates the dyes. Before we started, we made a hypothesis. Mine was if paper chromatography is done on the

traveled from the faint line on at the tip of the chromatography paper and it then created bands of colors. The original color of the plant extract was a black color and the once it was immersed in developer four it then started to travel up the paper strip. How do your Rf values compare with those of your classmates? They were similar but some were different due to errors in the experiment. List some other uses of chromatography? Chromatography can be used to separate pigments in fruit juices or