Thin layer chromatography Essays

  • Thin Layer Chromatography Essay

    1069 Words  | 5 Pages

    Introduction The purpose of this experiment was to identify the composition of over-the-counter analgesics by the method of Thin Layer Chromatography (TLC). The TLC method is used for rapid qualitative analysis of mixtures to determine and identify its components and purity. A development solvent was used to separate the analgesics found in both the known drug sample and an unknown sample of over-the-counter medications. The pertinent techniques for this experiment are spotting the stationary phase

  • Essay On Thin Layer Chromatography

    1608 Words  | 7 Pages

    Chromatographic analysis: Thin Layer Chromatography175 Procedure Thin layer chromatography is used for the separation, identification and quality control of drug. It also determines small amount of impurities or adulterants if present. Apparatus Glass plates of uniform thickness, 15-20 cm long and wide, spreader, chromatographic chamber of glass with a tightly fitting lid having suitable size to accommodate the glass plates and capillary, ultraviolet light source emitting short 254 nm and long

  • Thin Layer Chromatography Lab Report

    929 Words  | 4 Pages

    Thin Layer Chromatography (TLC) Abstract This experiment uses the TLC chromatography technique to identify the presence of acetylsalicylic and Acetaminophen in analgesic drugs (Tylenol and Anacin). It was found that the Anacin and acetylsalicylic had very closer Rf values (0.8 and 0.79). The Tylenol and acetaminophen had closer Rf values (0.54 and 0.58). Hence, Acetylsalicylic acid and acetaminophen were present in Anacin and Tylenol tablets respectively. Introduction Chromatography is the technique

  • Thin Layer Chromatography

    5279 Words  | 22 Pages

    under ultraviolet light or spraying the plate with a chemical reagent. The distance a spot travels up a thin-layer plate can be assigned a numerical value known as the Rf value. Theory: The technique of thin-layer chromatography (TLC) uses a solid stationary phase and a moving liquid phase to separate the constituent of a mixture. A thin-layer plate is prepared by coating a glass plate with a thin film of a granular material, usually silica gel or aluminium oxide. This granular material serves as the

  • Capsicum Using Column Chromatography Lab Report

    1086 Words  | 5 Pages

    Ritchmond P. Togonon, Abiather L. Tsai, Jordan Martin G. Uy Department of Sports Science, College of Rehabilitation Sciences * Abstract This experiment is about the separation of pigments in capsicum annuum using column chromatography. In this experiment pigments in capsicum annuum or red pepper were separated. Four different eluates with different colors were produced from the column by adding eluents through the column. Keywords: capsicum annuum, eluate, eluent, dichloromethane

  • Photosynthetic Pigments Research Paper

    826 Words  | 4 Pages

    In thin layer chromatography, the example is separated in the flexible stage dissolvable. The ensuing course of action is then spotted near the base of the rectangular sheet of glass or plastic secured with silica. The sheet is then submerged in a chamber containing a little measure of dissolvable, keeping the spots over the surface of the dissolvable. Once the sheet is set in the chamber, the dissolvable begins to increase on the sheet through thin movement. The covering on the sheet is the staionary

  • Spinach Lab Report

    756 Words  | 4 Pages

    Chromatography of Spinach Formal Discussion This lab involved the extraction of pigments from spinach leaves which were then analyzed using thin layer chromatography. The first step of this process was to grind up the leaves in order to extract the pigments. Hexanes facilitated this process and afterwards, the solution was dried over sodium sulfate to remove water. Next, the column was packed with a small piece of glass wool, followed by hexanes, and then packing sand. At this point, the column

  • Photosynthetic Pigments Lab Report

    1294 Words  | 6 Pages

    Photosynthetic Pigments Lab The aim of this lab is to separate photosynthetic pigments by chromatography, a method used to separate the components of a mixture, and to measure each pigment’s Rf value. There are different types of chromatography, and the one which this lab focuses on is paper chromatography. The components of the mixture, in this case, spinach leaves mixed with acetone, are pipetted at one end of the chromatography paper and hung in a jar slightly above solvent vapor to create the reaction.

  • Wheat Honey Research Paper

    934 Words  | 4 Pages

    3.1 Phenolics in buckwheat honey Figure 1 illustrates the wide variety of HPLC profiles of six buckwheat honey samples. As can be seen, these samples could be divided into two main types. The first type exhibited very similar chromatograms, including samples 1, 2, 3 and 4. The retention time of most compounds is 20 to 70 min. Conversely, the second type include sample 5 and 6. Their chromatograms appeared simpler and showed only a few lower level peaks. The figure showed the highest peak is

  • Biochemistry: The Importance Of Proteins

    1106 Words  | 5 Pages

    Proteins are the most abundant molecules in cells, making up 50% or more of their dry weight. Every protein has unique structure and conformation or shape, which enables it to carry out a specific function in a living cell. Proteins comprise the complex muscle system and the connective tissue network, and they are important as carriers in the blood system. All enzymes are proteins, enzymes All proteins contain carbon, hydrogen, nitrogen and oxygen. Most protein contain sulfur and some additional

  • Year 10 Forensics: Chromatography Investigation

    400 Words  | 2 Pages

    Year 10 Forensics Chromatography Investigation Background: The practical that was completed was to investigate and solve what the mystery ink was and to find this out the technique of chromatography was used. Chromatography is a technique used by crime scene investigators to help then solve the crime by matching inks, lipsticks etc. chromatography is the separation of a mixture by passing it in a solution through a medium in which different components travel at different speeds. In the practical

  • Chromatography Lab Report Discussion

    1458 Words  | 6 Pages

    Introduction The term chromatography actually means colour writing, and signifies a technique by which the substance to be examined is placed in a vertical glass tube containing an adsorbent, the different segments of the substance traveling through the adsorbent at distinctive rates of velocity, according to their degree of attraction to it, and producing bands of colour at different levels of the adsorption column. The substances least absorbed emerge earliest; those more strongly absorbed emerge

  • Gas Chromatography Essay

    1867 Words  | 8 Pages

    Chromatography means "color writing" literally from the Greek words chroma and graphe. It is a separating technique for a mixture of chemicals, which can be in gas or liquid form, by letting them creep slowly past another substance. There are two important things in chromatography that is it must has one state of matter such as gas or liquid, that is known as mobile phase, moving over the surface of another state of matter which can be liquid or solid that stays where it is that is known as stationary

  • Principle Of Chromatography Essay

    977 Words  | 4 Pages

    The chromatography term is derived from a Greek word Chromo for color and Graphe for writing. Chromatography is a separation technique based on the partitioning behavior. The concept of chromatography was introduced by a Russian botanist Mikhail Tswett in 1906. In this technique solute of interest is partitioned between two phases i.e. a mobile phase and a stationary phase depending on the partitioning value. The mobile phase includes the solvent and the stationary phase includes the column in

  • Column Chromatography Essay

    1137 Words  | 5 Pages

    (Carels and Shepherd 1977; Chen MH, 1993). Column chromatography For the isolation and purification of Monascus pigments, column chromatography had been extensively used from long time. However, its isolated pigments fraction is further need to purify by Thin Layer Chromatography and High Performance Liquid Chromatography (Vidyalakshmi et al. 2009). In 1975, Yoshimura M, et al. isolation, and purification of Monascus pigments through column chromatography by using Sephadex G-50 (3 cm × 5 cm) column with

  • Hexane Lab Report

    1619 Words  | 7 Pages

    affinity of the component towards the stationary phase. In general, the more polar component would have a stronger interaction with the stationary phase, and the less polar component would be eluted out first. From the observations of the column chromatography, yellow S2 collected is β-carotene, indicating that

  • Phospholipids Analysis

    855 Words  | 4 Pages

    methods used for the analysis of phospholipids. All systems of chromatography consist of a stationary and mobile phase. A monster placed on a stationary phase, i.e., a solid or a liquid, and the mobile phase, a gas or a liquid, is allowed by modifying the system. The components of the sample will be separated on the basis of their ranging physical and chemical properties, imparting different affinities for the two phases. Thin layer chromatography (TLC) was the first chromatographic method for assessing

  • Ketones Lab Report

    2072 Words  | 9 Pages

    was stirred for the appropriate reaction times at 80 °C (Table 2). After completion of the reaction (monitored by thin-layer chromatography, TLC), the mixture was diluted with Et2O and filtered. The organic layer was washed with 10% NaHCO3 solution and then dried over anhydrous Na2SO4. The solvent was evaporated under reduced pressure and the product purified by column chromatography on silica gel to give the corresponding pure aryl

  • Organic Modifier Case Study

    1433 Words  | 6 Pages

    3. Results and discussion 3.1. Optimization of chromatographic conditions A rapid, simple, reproducible and accurate RP-HPLC method was developed for simultaneous determination of pregabalin and atenolol in dosage forms, spiked and volunteer human urine. To optimize the proposed HPLC method; several parameters such as effect of organic modifier, pH of buffer and flow rate were studied. The effect of organic modifier: The percentage of organic modifier (methanol) has a critical effect on separation

  • Hair Extension Research Paper

    818 Words  | 4 Pages

    Hair Beauty Extension for Good, Beautiful and Healthy Hair Beauty attracts everyone and people are really influenced by this. African-American women are known for their plethora of diverse hairstyles. Every person desires to look beautiful and trendy. In order to look beautiful, hair extensions play an important part to make you look appealing and glamorous. Hair Extensions Staten Island NY can augment your looks and can give you an attractive personality. Everyone is born with natural hair which